A5029142611- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Lysosomal Storage Disorders Research
- Biochemical and Molecular Research
- Blood properties and coagulation
- Calcium signaling and nucleotide metabolism
- Bacterial Genetics and Biotechnology
- Genetic and Kidney Cyst Diseases
- Immune Cell Function and Interaction
- Platelet Disorders and Treatments
- Cardiomyopathy and Myosin Studies
- Mitochondrial Function and Pathology
- Pancreatic function and diabetes
- Escherichia coli research studies
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- CRISPR and Genetic Engineering
- Diet, Metabolism, and Disease
- Microtubule and mitosis dynamics
- Renal and related cancers
- Diabetes and associated disorders
- Biomedical Research and Pathophysiology
- Renal Diseases and Glomerulopathies
- Erythrocyte Function and Pathophysiology
- Nematode management and characterization studies
Université Libre de Bruxelles
2014-2025
Institute of Pathology and Genetics
2004-2020
University of Namur
2014
de Duve Institute
2008
Génomes, biologie cellulaire et thérapeutiques
2007
Laboratoire de Microbiologie et Génétique Moléculaires
2006-2007
Gembloux Agro-Bio Tech
2005
Trinity College Dublin
2005
Instituut voor Tropische Geneeskunde
2002
Vrije Universiteit Brussel
2002
Immortal spheroids were generated from fetal mouse intestine using the culture system initially developed to organoids adult intestinal epithelium. Spheroid proportion progressively decreases postnatal period, with a corresponding increase in production of organoids. Like organoids, show Wnt-dependent indefinite self-renewing properties but display poorly differentiated phenotype reminiscent incompletely caudalized progenitors. The spheroid transcriptome is strikingly different that stem...
BackgroundSARS-CoV-2 targets endothelial cells through the angiotensin-converting enzyme 2 receptor. The resulting injury induces widespread thrombosis and microangiopathy. Nevertheless, early specific markers of dysfunction vascular redox status in COVID-19 patients are currently missing.MethodsObservational study including ICU non-ICU adult admitted hospital for acute respiratory failure, compared with control subjects matched cardiovascular risk factors similar to patients, septic shock...
Apolipoprotein L-I is the trypanolytic factor of human serum. Here we show that this protein contains a membrane pore-forming domain functionally similar to bacterial colicins, flanked by membrane-addressing domain. In lipid bilayer membranes, apolipoprotein formed anion channels. Trypanosoma brucei, was targeted lysosomal and triggered depolarization membrane, continuous influx chloride, subsequent osmotic swelling lysosome until trypanosome lysed.
Multiple phosphatidylinositol (PtdIns) 3-kinases (PI3Ks) can produce PtdIns3P to control endocytic trafficking, but whether enzyme specialization occurs in defined subcellular locations is unclear. Here, we report that PI3K-C2α enriched the pericentriolar recycling compartment (PRE) at base of primary cilium, where it regulates production a specific pool PtdIns3P. Loss PI3K-C2α-derived leads mislocalization PRE markers such as TfR and Rab11, reduces Rab11 activation, blocks accumulation Rab8...
Tsetse flies are the sole vectors of Trypanosoma brucei parasites that cause sleeping sickness. Our knowledge on early interface between infective metacyclic forms and mammalian host skin is currently highly limited. Glossina morsitans infected with fluorescently tagged T. were used in this study to initiate natural infections mice. Metacyclic trypanosomes found be infectious through intradermal route sharp contrast blood stream form trypanosomes. Parasite emigration from dermal inoculation...
Abstract Humans resist infection by the African parasite Trypanosoma brucei owing to trypanolytic activity of serum apolipoprotein L1 (APOL1). Following uptake endocytosis in parasite, APOL1 forms pores endolysosomal membranes and triggers lysosome swelling. Here we show that induces both lysosomal mitochondrial membrane permeabilization (LMP MMP). Trypanolysis coincides with MMP consecutive release Tb EndoG endonuclease nucleus. is associated kinesin KIFC1, which motor vesicular trafficking...
Abstract Vertebrate organs require locally adapted blood vessels 1,2 . The gain of such organotypic vessel specializations is often deemed to be molecularly unrelated the process organ vascularization. Here, opposing this model, we reveal a molecular mechanism for brain-specific angiogenesis that operates under control Wnt7a/b ligands—well-known blood–brain barrier maturation signals 3–5 relies on Wnt7a/b-dependent expression Mmp25, which find enriched in brain endothelial cells. CRISPR–Cas9...
Progress in diagnosis, treatment, and epidemiology of human African trypanosomiasis (sleeping sickness) depends on the existence specific sensitive diagnostic tools. Inherent shortcomings serologic parasitologic methods can be overcome by molecular techniques. Therefore, we have developed a new polymerase chain reaction (PCR) test using primers derived from recently identified sequence Trypanosoma brucei gambiense-specific glycoprotein (TgsGP). The specificity TgsGP-PCR was evaluated DNA...
Protozoan Kinetoplastida, a group that comprises the pathogenic Trypanosoma brucei, compartmentalize several metabolic systems such as major part of glycolytic pathway, in multiple peroxisome-like organelles, designated glycosomes. Trypanosomes have complicated life cycle, involving two major, distinct stages living mammalian bloodstream and inhabiting different body parts tsetse fly. Previous studies on non-differentiating trypanosomes shown metabolism enzymatic contents glycosomes...
Interactions between chronic lymphocytic leukemia (CLL) B cells and the bone marrow (BM) microenvironment play a major function in physiopathology of CLL. Extracellular vesicles (EVs), which are composed exosomes microparticles, an important role cell communication. However, little is known about their CLL / interactions. In present study, EVs purified by ultracentrifugation from BM mesenchymal stromal (BM-MSC) cultures were added to cells. After integration into cells, we observed decrease...
The C-terminal variants G1 and G2 of apolipoprotein L1 (APOL1) confer human resistance to the sleeping sickness parasite Trypanosoma rhodesiense, but they also increase risk kidney disease. APOL1 APOL3 are death-promoting proteins that partially associated with endoplasmic reticulum Golgi membranes. We report in podocytes, either helix truncation (APOL1Δ) or deletion (APOL3KO) induces similar actomyosin reorganization linked inhibition phosphatidylinositol-4-phosphate [PI(4)P] synthesis by...
Abstract Approximately 20% of sleeping sickness patients exhibit respiratory complications, however, with a largely unknown role the parasite. Here we show that tsetse fly-transmitted Trypanosoma brucei parasites rapidly and permanently colonize lungs occupy extravascular spaces surrounding blood vessels alveoli bronchi. They are present as nests multiplying exhibiting close interactions collagen active secretion extracellular vesicles. The local immune response shows substantial increase...
Abstract The MIR 396 family, composed of ath‐miR396a and ath‐miR396b in A rabidopsis , is conserved among plant species known to target the Growth‐Regulating Factor ( GRF ) gene family. ath‐miR396 overexpressors or grf mutants are characterised by small narrow leaves show embryogenic defects such as cotyledon fusion. Heterologous expression has been reported tobacco resulted reduction three N t genes. In this study, precursor P opulus trichocarpa ptc‐miR396c with a mature sequence identical...
The African trypanosome Trypanosoma brucei, which persists within the bloodstream of mammalian host, has evolved potent mechanisms for immune evasion. Specifically, antigenic variation variant-specific surface glycoprotein (VSG) and a highly active endocytosis recycling coat efficiently delay killing mediated by anti-VSG antibodies. Consequently, conventional VSG-specific intact immunoglobulins are non-trypanocidal in absence complement. In sharp contrast, monovalent antigen-binding...
The oil-in-water emulsion Adjuvant System 03 (AS03) is one of the few adjuvants used in licensed vaccines. Previous work indicates that AS03 induces a local and transient inflammatory response contributes to its adjuvant effect. However, molecular mechanisms involved immunostimulatory properties are ill-defined. Upon intramuscular injection mice, elicited rapid downregulation lipid metabolism-related genes draining lymph node. In vitro, these modifications were associated with profound...
Abstract GPR85/SREB2 is an exceptionally well-conserved orphan 7TM receptor with unclear biological attributes. In this study, we used several zebrafish genetic models to investigate Gpr85 properties and functions throughout development adulthood. We describe that, like in mammals, gpr85 expressed by various neuronal populations adulthood the central nervous system, retina intestine. The overexpression of a fluorochrome-tagged version -expressing neurons provides first vivo evidence that...
Nanoparticle technology, particularly gold nanoparticles (AuNPs), is being developed for a wide range of applications, including as delivery system peptides or nucleic acids (NA). Their use in precision medicine requires detailed engineering NP functionalization to optimize their function and minimize off-target toxicity. Two main routes can be found the literature attachment NA strands AuNPs: covalent binding via thiol group passive adsorption onto specially adapted coating previously...