A5033434561- Bacterial Genetics and Biotechnology
- Blood properties and coagulation
- Genetic and Kidney Cyst Diseases
- Renal and related cancers
- Erythrocyte Function and Pathophysiology
- Genetic diversity and population structure
- Diet and metabolism studies
- Turtle Biology and Conservation
- Reproductive Biology and Fertility
- Platelet Disorders and Treatments
- CRISPR and Genetic Engineering
- Toxin Mechanisms and Immunotoxins
- Coral and Marine Ecosystems Studies
- Escherichia coli research studies
- Skin and Cellular Biology Research
- Plant Reproductive Biology
- DNA Repair Mechanisms
- Liver Disease Diagnosis and Treatment
- Biomedical Research and Pathophysiology
- Microtubule and mitosis dynamics
- Plant Genetic and Mutation Studies
- Wildlife Ecology and Conservation
- Diet, Metabolism, and Disease
- Hedgehog Signaling Pathway Studies
- interferon and immune responses
Université Libre de Bruxelles
2013-2024
Immortal spheroids were generated from fetal mouse intestine using the culture system initially developed to organoids adult intestinal epithelium. Spheroid proportion progressively decreases postnatal period, with a corresponding increase in production of organoids. Like organoids, show Wnt-dependent indefinite self-renewing properties but display poorly differentiated phenotype reminiscent incompletely caudalized progenitors. The spheroid transcriptome is strikingly different that stem...
Multiple phosphatidylinositol (PtdIns) 3-kinases (PI3Ks) can produce PtdIns3P to control endocytic trafficking, but whether enzyme specialization occurs in defined subcellular locations is unclear. Here, we report that PI3K-C2α enriched the pericentriolar recycling compartment (PRE) at base of primary cilium, where it regulates production a specific pool PtdIns3P. Loss PI3K-C2α-derived leads mislocalization PRE markers such as TfR and Rab11, reduces Rab11 activation, blocks accumulation Rab8...
Myzostomids are obligate symbiotic invertebrates associated with echinoderms a fossil record that extends to the Ordovician period. Due their long history as host-specific symbionts, myzostomids have acquired unique anatomy obscures phylogenetic affinities other metazoans: they incompletely segmented, parenchymous, acoelomate organisms chaetae and trochophore larva. Today, most often classified within annelids either an aberrant family of polychaetes or separate class. We inferred position...
Abstract The MIR 396 family, composed of ath‐miR396a and ath‐miR396b in A rabidopsis , is conserved among plant species known to target the Growth‐Regulating Factor ( GRF ) gene family. ath‐miR396 overexpressors or grf mutants are characterised by small narrow leaves show embryogenic defects such as cotyledon fusion. Heterologous expression has been reported tobacco resulted reduction three N t genes. In this study, precursor P opulus trichocarpa ptc‐miR396c with a mature sequence identical...
Signaling from the primary cilium regulates kidney tubule development and cyst formation. However, mechanism controlling targeting of ciliary components necessary for morphogenesis signaling is largely unknown. Here, we studied function class II phosphoinositide 3-kinase-C2α (PI3K-C2α) in renal tubule-derived inner medullary collecting duct 3 cells show that PI3K-C2α resides at recycling endosome compartment proximity to base. In this subcellular location, controlled activation Rab8, a key...
Apolipoproteins L (ApoLs) are Bcl‐2‐like proteins expressed under inflammatory conditions in myeloid and endothelial cells. We found that Toll‐like receptor (TLR) stimuli, particularly the viral mimetic polyinosinic:polycytidylic acid (poly(I:C)), specifically induce ApoLs7/11 subfamilies murine CD8α + dendritic cells (DCs). This induction requires TLR3/TRIF (where TRIF is TIR domain containing adapter‐inducing interferon β) signaling pathway dependent on IFN‐β all ApoLs except for ApoL7c ....
Acute respiratory distress syndrome due to coronavirus disease 2019 (COVID-19) is associated with high mortality. Several studies have reported that the microcirculation responds adequately hypoxia in COVID-19 patients by increasing oxygen availability, contrast inadequate response observed bacterial sepsis. Red blood cells (RBCs), key for transport, and notably their rheology, are altered during sepsis, but few data available COVID-19.In this prospective, non-interventional study, shape was...
As natural populations of endangered species dwindle to precarious levels, remaining members are sometimes brought into captivity, allowed breed and their offspring returned the habitat. One goal such repatriation programmes is retain as much genetic variation possible. A taxon giant Galápagos tortoises on island Española has been subject a captive breeding–repatriation programme for 33 years. Core breeders, consisting 12 females three males, have produced more than 1200 that released where...
Ruthenium(ii) complexes are attracting significant research attention as a promising class of photosensitizers (PSs) in photodynamic therapy (PDT). Having previously reported the synthesis two novel Ru(ii)-polypyridyl-1,8-naphthalimide Tröger's base compounds 1 and 2 with interesting photophysical properties, where emission from either Ru(ii) polypyridyl centres or naphthalimide moieties could be used to monitor binding nucleic acids, we sought use these investigate further more detail their...
ABSTRACT Programmed cell death (PCD) is an important hallmark of multicellular organisms. Cells self-destruct through a regulated series events for the benefit organism as whole. The existence PCD in bacteria has long been controversial due to widely held belief that only organisms would profit from this kind altruistic behavior at cellular level. However, over past decade, compelling experimental evidence established such pathways bacteria. Here, we report expression mutant isoform...
Giant Galápagos tortoises on the island of Española have been focus an intensive captive breeding-repatriation programme for over 35 years that saved taxon from extinction. However, analysis 118 samples released individuals indicated bias sex ratio and large variance in reproductive success among 15 breeders has severely reduced effective population size (N e ). We report here additional 473 captive-bred back to reveals individual (E1465) exhibits nuclear microsatellite alleles not found any...
ABSTRACT During the course of mammalian infection, African trypanosomes undergo extensive cellular differentiation, as actively dividing long slender (SL) forms progressively transform into intermediate (I) and finally quiescent G 1 /G 0 -locked short stumpy (ST) forms. ST maintain adaptations compatible with their survival in bloodstream, such high endocytic activity, but they already show preadaptations to insect midgut conditions. The nutritional requirements must differ from those SL...
Cell division is a vital part of the cell cycle that fundamental to all life. Despite decades intense investigation, this process still incompletely understood. Previously, essential GTPase ObgE, which plays role in myriad basic cellular processes (such as initiation DNA replication, chromosome segregation and ribosome assembly), was proposed act checkpoint Escherichia coli by licensing segregation. We here describe effect mutant isoform ObgE (ObgE*) causes death irreversible arrest at stage...
Medical implants have improved the quality of life many patients. However, surgical intervention may eventually lead to implant microbial contamination. The aims this research were develop an easy, robust, quantitative assay assess surface antimicrobial activities, especially anti-nascent biofilm activity, and identify control surfaces, allowing for international comparisons. Using new assays inhibition nascent during persistent contact or after transient with bacteria, we show that 5 cent...
The ability to respond mechanical forces is a basic requirement for maintaining endothelial cell (ECs) homeostasis, which continuously subjected low shear stress (LSS) and high (HSS). In arteries, LSS HSS have differential impact on EC autophagy processes. However, it still unclear whether differently tune unique autophagic machinery or trigger specific responses in ECs.
Abstract The virulence of Shigella mainly resides in the use a Type 3 Secretion System (T3 SS ) to inject several proteins inside host cell. Three categories are hierarchically secreted: (1) needle components (MxiH and MxiI), (2) translocator which form pore (translocon) cell membrane, (3) effectors interfering with signaling pathways. In absence contact, T3 is maintained an “off” state by presence tip complex. We have previously identified gatekeeper protein, MxiC, sequesters bacteria...
Background/Aims: The Escherichia coli MazF is an endoribonuclease that cleaves mRNA at ACA sequences, thereby triggering inhibition of protein synthesis. aim this study to evaluate the efficiency mazEF toxin-antitoxin system in plants develop biotechnological tools for targeted cell ablation. Methods: A double transformation strategy, combining expression mazE antitoxin gene under control CaMV 35S promoter, reported drive all plant cells except within tapetum, together with mazF TA29...
The two species of Galápagos land iguanas (Conolophus subcristatus and C. pallidus) are listed as 'vulnerable' by the International Union for Conservation Nature (IUCN Red List; http://www.iucnredlist.org). Here, we report on isolation characterization 10 microsatellite markers using 562 individuals sampled all islands where Conolophus occur today. We show that these loci highly polymorphic display diagnostic alleles five out six island populations. These will be useful population genetic...
ABSTRACT Background and Aims Metabolic dysfunction-associated steatohepatitis (MASH) is a progressive liver disease that can lead to fibrosis, cirrhosis, hepatocellular carcinoma. Though MASH closely tied metabolic risk factors, the underlying pathogenic mechanisms remain scarcely understood. Recent research underscores importance of gut-liver axis in its pathogenesis, an aspect less explored human studies. Here, we investigated whether duodenal epithelium patients, could exhibit intrinsic...
It has been observed in vitro that complete clot lysis is generally preceded by a slow phase of during which the degradation seems to be inefficient. However, this regime was merely noticed, but not yet quantitatively discussed. In our experiments, we ubiquitously occurred two distinct regimes, and fast regime. We quantified extensively duration these regimes for wide spectrum experimental conditions found on average, lasts longer than one, meaning most process, ineffective. proposed...