A5033759386- Plant tissue culture and regeneration
- Plant-Microbe Interactions and Immunity
- Cancer Treatment and Pharmacology
- Phytochemical compounds biological activities
- Plant Pathogens and Fungal Diseases
- Plant Gene Expression Analysis
- Legume Nitrogen Fixing Symbiosis
- Plant biochemistry and biosynthesis
- Plant Pathogenic Bacteria Studies
- Mycorrhizal Fungi and Plant Interactions
- Microbial Natural Products and Biosynthesis
- Bacterial biofilms and quorum sensing
- Pineapple and bromelain studies
- Essential Oils and Antimicrobial Activity
- Bioenergy crop production and management
- Plant nutrient uptake and metabolism
- Polysaccharides and Plant Cell Walls
- Transgenic Plants and Applications
- Biofuel production and bioconversion
- Plant Molecular Biology Research
- Plant Reproductive Biology
- Toxin Mechanisms and Immunotoxins
- Antibiotic Resistance in Bacteria
- Ubiquitin and proteasome pathways
- Chemical synthesis and alkaloids
Université Libre de Bruxelles
2016-2025
Universidad Libre de Colombia
2021
Laboratoire de Recherche en Sciences Végétales
2020
Laboratoire de Biotechnologie de l'Environnement
2012-2016
Instituto de Fisiología Vegetal
2015
Vlaams Instituut voor Biotechnologie
2001
Ghent University
2001
National Institute of Health Sciences
1993-1994
Ca' Foscari University of Venice
1988
ABSTRACT Quorum-sensing (QS) regulates the production of key virulence factors in Pseudomonas aeruginosa and other important pathogenic bacteria. In this report, extracts leaves bark Combretum albiflorum (Tul.) Jongkind ( Combretaceae ) were found to quench QS-dependent P. PAO1. Chromatographic fractionation crude active extract generated several fractions containing flavonoids, as shown by their typical spectral features. Purification structural characterization one compounds led...
Preliminary screening of the Malagasy plant Combretum albiflorum for compounds attenuating production quorum sensing (QS)-controlled virulence factors in bacteria led to identification active fractions containing flavonoids. In present study, several flavonoids belonging flavone, flavanone, flavonol and chalcone structural groups were screened their capacity reduce QS-controlled opportunistic pathogen Pseudomonas aeruginosa (strain PAO1). Flavanones (i.e. naringenin, eriodictyol taxifolin)...
The role and metabolism of indole-3-acetic acid in gram-negative bacteria is well documented, but little known about biosynthesis regulation gram-positive bacteria. phytopathogen Rhodococcus fascians, a organism, incites diverse developmental alterations, such as leafy galls, on wide range plants. Phenotypic analysis gall suggests that auxin may play an important the development symptoms. We show here for first time R. fascians produces secretes acid. Interestingly, whereas...
Recently, extracts of Dalbergia trichocarpa bark have been shown to disrupt P. aeruginosa PAO1 quorum sensing (QS) mechanisms, which are key regulators virulence factor expression and implicated in biofilm formation. One the active compounds has isolated identified as oleanolic aldehyde coumarate (OALC), a novel bioactive compound that inhibits formation its maintenance well las rhl QS systems. Consequently, production QS-controlled factors including, rhamnolipids, pyocyanin, elastase...
Abstract The MIR 396 family, composed of ath‐miR396a and ath‐miR396b in A rabidopsis , is conserved among plant species known to target the Growth‐Regulating Factor ( GRF ) gene family. ath‐miR396 overexpressors or grf mutants are characterised by small narrow leaves show embryogenic defects such as cotyledon fusion. Heterologous expression has been reported tobacco resulted reduction three N t genes. In this study, precursor P opulus trichocarpa ptc‐miR396c with a mature sequence identical...
An indigenous consortium of arbuscular mycorrhizal fungi (AMF) from the Figuig oasis in southern Morocco, comprising Rhizophagus sp., Funneliformis Acaulospora Sclerocystis and Scutellospora was evaluated for its effects on growth sensitivity Phoenix dactylifera L. variety (cv. Boufeggous Gharas) to Fusarium oxysporum f. sp. Albedinis (Foa), causal agent Bayoud disease. Despite high fruit quality local appreciation, Gharas is highly susceptible Foa currently at risk extinction, underscoring...
An immunoassay for detection of a specific genetically modified soybean (Roundup-Ready) was validated on dried powder in an interlaboratory study. Different percentages soybeans nonmodified matrix were evaluated blind Thirty-eight laboratories from 13 countries participated. The 2 endpoints: (1) To give semiquantitative result, i.e., determination given sample above or below threshold, (2) to compute quantitative percentage the sample. Semiquantitative results showed that which contained <2%...
Members of Gram-positive Actinobacteria cause economically important diseases to plants. Within the Rhodococcus genus, some members can growth deformities and persist as pathogens on a wide range host The current model predicts that phytopathogenic isolates require cluster three loci present linear plasmid, with fas operon central virulence. Fas proteins synthesize, modify, activate mixture regulating cytokinins, which hormonal imbalance in plants, resulting abnormal growth. We sequenced...
Monolignols are the building blocks for lignin polymerization in apoplastic domain. Monolignol biosynthesis, transport, storage, glycosylation, and deglycosylation main biological processes partaking their homeostasis. In Arabidopsis thaliana, members of uridine diphosphate-dependent glucosyltransferases UGT72E UGT72B subfamilies have been demonstrated to glycosylate monolignols. Here, poplar UGT72 family, which is clustered into four groups, was characterized: Group 1 UGT72AZ1 UGT72AZ2,...
Rhodococcus fascians is a plant-pathogenic bacterium that causes malformations on aerial plant parts, whereby leafy galls occur at axillary meristems. The colonization behavior Nicotiana tabacum and Arabidopsis thaliana plants was examined. Independent of the infection methods, R. extensively colonized surface where bacteria were surrounded by slime layer. caused collapse epidermal cells penetrated intercellularly into tissues. onset symptom development preceded extensive interior....
ABSTRACT The gram-positive plant pathogen Rhodococcus fascians provokes leafy gall formation on a wide range of plants through secretion signal molecules that interfere with the hormone balance host. Crucial virulence genes are located linear plasmid, and their expression is tightly controlled. A mutant mutation in chromosomal locus affected was isolated. gene vicA , which encodes malate synthase functional glyoxylate shunt Krebs cycle. VicA required for efficient planta growth symptomatic,...
Rhodococcus fascians is currently the only phytopathogen of which virulence genes occur on a linear plasmid. To get insight into origin this replicon and strategy broad-spectrum phytopathogen, sequence plasmid strain D188, pFiD188, was determined. Analysis 198,917 bp revealed four syntenic regions with plasmids R. erythropolis, jostii, opacus, suggesting common these replicons. Mutational analysis pFi_086 pFi_102, similar to cutinases type IV peptidases, respectively, showed that conserved...