A5047057020- Plant Gene Expression Analysis
- Phytochemicals and Antioxidant Activities
- Plant biochemistry and biosynthesis
- Microbial Natural Products and Biosynthesis
- Horticultural and Viticultural Research
- Berry genetics and cultivation research
- Biochemical and biochemical processes
- Plant Stress Responses and Tolerance
- Plant Molecular Biology Research
- Fermentation and Sensory Analysis
- Plant tissue culture and regeneration
- Marine Sponges and Natural Products
- Plant Parasitism and Resistance
- Genomics and Phylogenetic Studies
- Plant-Microbe Interactions and Immunity
- Organometallic Complex Synthesis and Catalysis
- Cytomegalovirus and herpesvirus research
- Influenza Virus Research Studies
- Antioxidant Activity and Oxidative Stress
- RNA and protein synthesis mechanisms
- Photosynthetic Processes and Mechanisms
- Analytical Chemistry and Chromatography
- Natural product bioactivities and synthesis
- Polysaccharides and Plant Cell Walls
- Plant nutrient uptake and metabolism
Technical University of Munich
2016-2025
University Hospital Ulm
2022-2024
Universitätsklinik für Hals-, Nasen- und Ohrenheilkunde
2020-2024
University and Rehabilitation Clinics Ulm
2021
Muhlenberg College
2019
Saarland University
2010-2018
Helmholtz Centre for Infection Research
2012-2018
Helmholtz Institute for Pharmaceutical Research Saarland
2012-2018
German Center for Infection Research
2018
University of Montana
2015-2017
This work characterized the role of R2R3-MYB10 transcription factor (TF) in strawberry fruit ripening. The expression this TF takes place mainly receptacle and is repressed by auxins activated abscisic acid (ABA), parallel to ripening process. Anthocyanin was not produced when FaMYB10 transiently silenced receptacles. An increase observed water-stressed fruits, which accompanied an both ABA anthocyanin content. High-throughput transcriptomic analyses performed fruits with downregulated...
Some bacterial clades are important sources of novel bioactive natural products. Estimating the magnitude chemical diversity available from such a resource is complicated by issues including cultivability, isolation bias and limited analytical data sets. Here we perform systematic metabolite survey ~2300 strains order Myxococcales, well-established source products, using mass spectrometry. Our analysis encompasses both known previously unidentified metabolites detected under laboratory...
A noninvasive, cell-autonomous reporter system was developed to monitor the generation and distribution of physiologically active pools abscisic acid (ABA). ABA response (abi1-1) biosynthesis (aba2-1) mutants Arabidopsis (Arabidopsis thaliana) were used validate in presence absence water stress. In stress, low levels ABA-dependent activation observed columella cells quiescent center root as well vascular tissues stomata cotyledons, suggesting a nonstress-related role for these cell types....
Abstract Reactive oxygen species are potentially damaging molecules. An important function of antioxidants is to intercept harmful triplet states, in order prevent the formation singlet oxygen, or quench directly. However, also reactive towards other active such as hydroxyl radical, superoxide anion and non‐excited ground state presence radical initiators. It well known that flavonoids carotenoids show strong antioxidant properties. Polyenes best among compounds by efficient energy transfer....
Intron-containing constructs encoding self-complementary 'hairpin' RNA (ihpRNA) have the potential to efficiently silence genes in a range of plant species. In this study we demonstrate silencing ripening-related chalcone synthase (CHS) gene strawberry fruits (Fragaria x ananassa cv. Elsanta) by construct containing partial sense and corresponding antisense sequences CHS separated an intron obtained from F. quinone oxidoreductase gene. An Agrobacterium strain carrying T-DNA expressing ihpRNA...
Strawberry (Fragaria x ananassa) fruit contains several anthocyanins that give the ripe fruits their attractive red color. The enzyme catalyzes formation of first stable intermediate in anthocyanin pathway is anthocyanidin-3-O-glucosyltransferase. A putative glycosyltransferase sequence (FaGT1) was cloned from a strawberry cDNA library and recombinant FaGT1 transferred UDP-glucose to anthocyanidins and, lesser extent, flavonols, generating respective 3-O-glucosides. Quantitative polymerase...
Flavonols, phenylalanine-derived secondary metabolites, have protective and regulatory functions in plants. In Arabidopsis thaliana, they are consecutively glycosylated at their 3-OH 7-OH groups. UGT78D1 UGT78D2 the major flavonol 3-O-glycosyltransferases leaves. The ugt78d1 ugt78d2 double mutant, which was strongly compromised initial 3-O-glycosylation, showed a severe specific repression of biosynthesis, retaining only one-third wild-type level. This metabolic phenotype associated with...
As nascent polypeptide chains are synthesized, they pass through a tunnel in the large ribosomal subunit. Interaction between specific and is used to induce translational stalling for regulation of gene expression. One well-characterized example Escherichia coli SecM (secretion monitor) product, which induces up-regulate translation initiation downstream secA gene, needed protein export. Although many key components have been identified, understanding mechanism by peptidyl transferase center...
Short-chain esters contribute to the blend of volatiles that define strawberry aroma. The last step in their biosynthesis involves an alcohol acyltransferase catalyses esterification acyl moiety acyl-CoA with alcohol. This study identified a novel gene (FaAAT2) whose expression pattern during fruit receptacle growth and ripening is accordance production throughout ripening. full-length FaAAT2 cDNA was cloned expressed Escherichia coli its activity analysed substrates. semi-purified enzyme...
Abstract Plant phenolics have drawn increasing attention due to their potential nutritional benefits. Although the basic reactions of biosynthetic pathways in plants been intensively analyzed, regulation accumulation and flux through pathway is not that well established. The aim this study was use a strawberry (Fragaria × ananassa) microarray investigate gene expression patterns associated with phenylpropanoids, flavonoids, anthocyanins fruit. An examination transcriptome, coupled metabolite...
Tandem mass spectrometry is a widely applied and highly sensitive technique for the discovery characterization of microbial natural products such as secondary metabolites from myxobacteria. Here, data mining workflow based on MS/MS precursor lists targeting only signals related to bacterial metabolism established using LC-MS crude extracts shaking flask fermentations. The devised method not biased toward specific compound classes or structural features capable increasing information content...
The major birch pollen allergen Bet v 1 is the main elicitor of airborne type I allergies and belongs to PR-10 family (pathogenesis-related proteins 10). most extensively studied allergen, well characterized at a biochemical immunological level; however, its physiological function remains elusive. In present study, we identify Q3OS (quercetin-3-O-sophoroside) as natural ligand 1. We isolated bound from mature confirmed binding by reconstitution 1-Q3OS complex. Fluorescence UV-visible...
Fruit softening in Fragaria (strawberry) is proposed to be associated with the modification of cell wall components such as xyloglucan by action wall-modifying enzymes. This study focuses on vitro and vivo characterization two recombinant endotransglucosylase/hydrolases (XTHs) from vesca, FvXTH9 FvXTH6. Mining publicly available F. vesca genome sequence yielded 28 putative XTH genes. showed highest expression level all FvXTHs a fruit transcriptome data set was selected closely related FvXTH6...
Systemic acquired resistance (SAR) is a form of inducible disease that depends on salicylic acid and its upstream regulator ENHANCED DISEASE SUSCEPTIBILITY1 ( EDS1 ). Although local Arabidopsis thaliana defence responses activated by the Pseudomonas syringae effector protein AvrRpm1 are intact in eds1 mutant plants, SAR signal generation abolished. Here, SAR-specific phenotype utilized to identify metabolites contribute SAR. To this end, bioassay-assisted fractionation extracts from wild...
Summary Strawberry ( Fragaria × ananassa ) is a fruit crop with distinct biphasic flavonoid biosynthesis. Whereas, in the immature receptacle, high levels of proanthocyanidins accumulate, which are associated herbivore deterrence and pathogen defense, prominent color‐giving anthocyanins primarily produced ripe ‘fruits’ helping to attract herbivores for seed dispersal. Here, constitutive experimental down‐regulation one branch proanthocyanidin biosynthesis was performed. As result, proportion...
Genetic analysis of biosynthetic gene clusters is becoming an accepted tool to predict the stereochemical outcome a biosynthesis. However, in case pellasoren, one chiral center was not predicted correctly. The absolute configuration verified by total synthesis, which also demonstrated that stereoselective protonations can be successfully applied natural products synthesis. Detailed facts importance specialist readers are published as "Supporting Information". Such documents peer-reviewed,...
Precise and fluent genetic manipulation is still limited to only a few prokaryotes. Ideally the highly advanced technologies available in Escherichia coli could be broadly applied. Our efforts apply lambda Red technology, widely termed 'recombineering', Photorhabdus Xenorhabdus yielded success. Consequently we explored properties of an endogenous luminescens Red-like operon, Plu2934/Plu2935/Plu2936. Bioinformatic functional tests indicate that Plu2936 5'-3' exonuclease equivalent Redα...
Eugenol is a volatile that serves as an attractant for pollinators of flowers, acts defense compound in various plant tissues, and contributes to the aroma fruits. Its production cultivated species such strawberry (Fragaria × ananassa), therefore, important viability quality fruit. We have identified functionally characterized three complementary DNAs (cDNAs) encode proteins with high identity eugenol synthases from several species. Based on sequence comparison wild relative Fragaria vesca,...
Vinification of grapes (Vitis vinifera) exposed to forest fire smoke can yield unpalatable wine due the presence taint compounds from and release derived volatiles their respective glycosides during fermentation process or in-mouth consumption. To identify glycosyltransferases (GTs) involved in formation glycosidically bound smoke-derived we performed gene expression analysis candidate GTs different grapevine tissues. Second, substrates bushfire naturally occurring were screened with...