A5036660533- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Plant-Microbe Interactions and Immunity
- Plant nutrient uptake and metabolism
- Plant Reproductive Biology
- Plant Gene Expression Analysis
- Photosynthetic Processes and Mechanisms
- Polysaccharides and Plant Cell Walls
- Legume Nitrogen Fixing Symbiosis
- Plant Pathogenic Bacteria Studies
- Bacterial Genetics and Biotechnology
- Plant tissue culture and regeneration
- Bacteriophages and microbial interactions
- Plant Virus Research Studies
- Genomics and Phylogenetic Studies
- Botanical Research and Chemistry
- Plant Genetic and Mutation Studies
- Epigenetics and DNA Methylation
- Biochemical Analysis and Sensing Techniques
- DNA Repair Mechanisms
- Enterobacteriaceae and Cronobacter Research
- Plant and animal studies
- Light effects on plants
- Soybean genetics and cultivation
- Insect and Pesticide Research
Anhalt University of Applied Sciences
2021-2024
Technical University of Munich
2014-2023
Austrian Academy of Sciences
2006-2021
Gregor Mendel Institute of Molecular Plant Biology
2006-2021
Max Perutz Labs
2006-2014
University of Vienna
2005-2014
Horticultural Crops Research Laboratory
2013
Vienna Biocenter
2006-2009
Transgenerational epigenetic inheritance has been defined by the study of relatively few loci. We examined a population recombinant inbred lines with epigenetically mosaic chromosomes consisting wild-type and CG methylation-depleted segments (epiRILs). Surprisingly, transposons that were immobile in parental displayed stochastic movement 28% epiRILs. Although analysis after eight generations inbreeding, supported genome-wide DNA methylation profiling, identified recombined chromosomal...
Plant growth and development are highly regulated processes that coordinated by hormones including the brassinosteroids (BRs), a group of steroids with structural similarity to steroid mammals. Although it is well understood how BRs produced their signals transduced, BR targets, which directly confer hormone's growth-promoting effects, have remained largely elusive. Here, we show regulate biosynthesis gibberellins (GAs), another class hormones, in Arabidopsis thaliana. We reveal mutants...
Brassinosteroids (BRs) are growth-promoting plant hormones that play a role in abiotic stress responses, but molecular modes enable this activity remain largely unknown. Here we show BRs participate the regulation of freezing tolerance. BR signaling-defective mutants Arabidopsis thaliana were hypersensitive to before and after cold acclimation. The constitutive activation signaling, contrast, enhanced resistance. Evidence is provided BR-controlled basic helix-loop-helix transcription factor...
Plant mitogen-activated protein kinases (MAPKs) are involved in important processes, including stress signaling and development. In a functional yeast screen, we identified mutations that render Arabidopsis thaliana MAPKs constitutively active (CA). Importantly, CA-MAPKs maintain their specificity toward known activators substrates. As proof-of-concept, MAPK4 (MPK4) function plant immunity was investigated. agreement with the phenotype of mpk4 mutants, CA-MPK4 plants were compromised...
Brassinosteroids (BRs) are steroid hormones that coordinate fundamental developmental programs in plants. In this study we show addition to the well established roles of BRs regulating cell elongation and division events, also govern fate decisions during stomata development Arabidopsis thaliana. wild-type A. thaliana, stomatal distribution follows one-cell spacing rule; is, adjacent spaced by at least one intervening pavement cell. This rule is interrupted BR-deficient BR...
Diverse stresses such as high salt conditions cause an increase in reactive oxygen species (ROS), necessitating a redox stress response. However, little is known about the signaling pathways that regulate antioxidant system to counteract oxidative stress. Here, we show Glycogen Synthase Kinase3 from Arabidopsis thaliana (ASKα) regulates tolerance by activating Glc-6-phosphate dehydrogenase (G6PD), which essential for maintaining cellular balance. Loss of stress-activated ASKα leads reduced...
Sugar and organic acid contents are major factors for tomato fruit flavour important breeding traits. Here we provide an improved protocol accurate quantification of the main sugars, glucose fructose, acids, citric malic acid, present in tomato. The extract is spiked with lactose tricarballylic as internal standards loaded onto a NH2 solid phase extraction (SPE) column. sugars appear flow-through subsequently analysed by HPLC using Nucleodur column refractive index detector. acids bind to...
Hydroponic cultivation of vegetables avoids problems with soil-borne plant pathogens and may allow higher yield. In arid climates particularly on islands, high concentrations sodium chloride can be present in the groundwater. For instance, many sites Malta, groundwater contains more than 10 mM chloride. Here we investigated effects at levels typically found Malta yield, physiology fruit quality tomato, economically most important vegetable. We selected cherry tomatoes since their production...
Stem cells are essential for the development and organ regeneration of multicellular organisms, so their infection by pathogenic viruses must be prevented. Accordingly, mammalian stem highly resistant to viral due dedicated antiviral pathways including RNA interference (RNAi). In plants, a small group harbored within shoot apical meristem generate all postembryonic above-ground tissues, germline cells. Many do not proliferate in these cells, yet molecular bases this exclusion remain only...
Covalent modification by methylation of cytosine residues represents an important epigenetic hallmark. While sequence analysis after bisulphite conversion allows correlative analyses with single-base resolution, functional interference DNA is less precise, due to the complexity enzymes and their targets. A cytidine analogue, 5-azacytidine, frequently used as inhibitor methyltransferases, but its rapid degradation in aqueous solution problematic for culture periods longer than a few hours....
Abstract Background Brassinosteroids (BRs) are signaling molecules that play essential roles in the spatial regulation of plant growth and development. In contrast to other hormones BRs act locally, close sites their synthesis, thus homeostatic mechanisms must operate at cellular level equilibrate BR concentrations. Whilst it is recognized levels bioactive likely adjusted by controlling relative rates biosynthesis catabolism, few factors, which participate these regulatory events, have as...
Brassinosteroids (BRs) are plant hormones that regulate many processes including cell elongation, leaf development, pollen tube growth and xylem differentiation. GSK3/shaggy-like kinases (GSK) critical regulators of intracellular signalling initiated by the binding BR to BRI1 receptor complex. Three GSKs have already been shown relay responses, phosphorylation transcriptional regulator BES1. However, recent studies indicate one or more yet unidentified protein involved in signalling. Here,...
Fruit softening in Fragaria (strawberry) is proposed to be associated with the modification of cell wall components such as xyloglucan by action wall-modifying enzymes. This study focuses on vitro and vivo characterization two recombinant endotransglucosylase/hydrolases (XTHs) from vesca, FvXTH9 FvXTH6. Mining publicly available F. vesca genome sequence yielded 28 putative XTH genes. showed highest expression level all FvXTHs a fruit transcriptome data set was selected closely related FvXTH6...
The ninhydrin reaction is commonly used for the detection of amino acids. However, in literature, different conditions with respect to buffer system, its pH and concentration, type organic solvent, incubation time, temperature, as well concentrations reagents, are described. To identify most suitable conditions, colour development reagents varying compositions temperatures times were investigated using asparagine a model acid. Asparagine was selected since it one abundant free acids many...
Abstract Epigenetic changes of gene expression can potentially be reversed by developmental programs, genetic manipulation, or pharmacological interference. However, a case transcriptional silencing, originally observed in tetraploid Arabidopsis thaliana plants, created an epiallele resistant to many mutations inhibitor treatments that activate other suppressed genes. This raised the question about molecular basis this extreme stability. A combination forward and reverse genetics drug...
Glycogen synthase kinase 3 (GSK-3) was originally identified as a regulator of glycogen synthesis in mammals. Like starch plants, is polymer glucose, and serves an energy carbon store. Starch the main carbohydrate store plants. Regulation metabolism, particular response to environmental cues, primary importance for flow plants but still obscure. Here, we provide evidence that MsK4, novel Medicago sativa GSK-3-like kinase, connects stress signalling with metabolism. MsK4 found be...
Summary Plants detect pathogens by sensing microbe‐associated molecular patterns ( MAMP s) through pattern recognition receptors. Pattern receptor complexes also have roles in cell death control, but the underlying mechanisms are poorly understood. Here, we report isolation of cerk1 ‐4, a novel mutant allele A rabidopsis chitin CERK 1 with enhanced defense responses. We identified ‐4 forward genetic screen barley powdery mildew and consequently characterized it pathogen assays, crosses...
Summary Enolases are key glycolytic enzymes that highly conserved in prokaryotic and eukaryotic organisms, among the most abundant cytosolic proteins. In this study we provide evidence activity of enolase ENO 2 is essential for growth development plants. We show Arabidopsis plants with compromised function, which were generated by mutating LOS 2/ locus, have severe cellular defects, including reduced cell size defective differentiation restricted lignification. At tissue organ level...