A5106085936- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant Stress Responses and Tolerance
- Plant-Microbe Interactions and Immunity
- Plant Micronutrient Interactions and Effects
- Chromosomal and Genetic Variations
- Plant nutrient uptake and metabolism
- Legume Nitrogen Fixing Symbiosis
- Polysaccharides and Plant Cell Walls
- Plant Disease Resistance and Genetics
- Plant Pathogenic Bacteria Studies
- Wheat and Barley Genetics and Pathology
- Plant responses to water stress
- Photosynthetic Processes and Mechanisms
- Plant Parasitism and Resistance
- Soybean genetics and cultivation
- Plant Gene Expression Analysis
- Genetic Mapping and Diversity in Plants and Animals
- Plant tissue culture and regeneration
- Agronomic Practices and Intercropping Systems
- Pesticide and Herbicide Environmental Studies
- Horticultural and Viticultural Research
- Plant Genetic and Mutation Studies
- Plant Pathogens and Fungal Diseases
- Genetic and Environmental Crop Studies
Université Paris-Saclay
2016-2025
Institut des Sciences des Plantes de Paris Saclay
2016-2025
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2025
Centre National de la Recherche Scientifique
2014-2025
Université Paris Cité
2015-2023
Université Paris-Sud
2015-2022
Sorbonne Paris Cité
2017-2022
Sorbonne Université
2018
Université d'Évry Val-d'Essonne
2013-2015
Institut National de la Recherche Agronomique
2004
Summary Abscisic acid ( ABA ) is a major phytohormone involved in important stress‐related and developmental plant processes. Recent phosphoproteomic analyses revealed large set of ‐triggered phosphoproteins as putative mitogen‐activated protein kinase MAPK targets, although the evidence for s signalling still scarce. Here, we identified reconstituted vivo complete ‐activated cascade, composed MAP 3Ks 3K17/18, 2K MKK 3 four C group MPK 1/2/7/14. In planta , show that activation 7 blocked...
Plant mitogen-activated protein kinases (MAPKs) are involved in important processes, including stress signaling and development. In a functional yeast screen, we identified mutations that render Arabidopsis thaliana MAPKs constitutively active (CA). Importantly, CA-MAPKs maintain their specificity toward known activators substrates. As proof-of-concept, MAPK4 (MPK4) function plant immunity was investigated. agreement with the phenotype of mpk4 mutants, CA-MPK4 plants were compromised...
Pyoverdines are siderophores synthesized by fluorescent Pseudomonas spp. Under iron-limiting conditions, these high-affinity ferric iron chelators excreted bacteria in the soil to acquire iron. produced beneficial ameliorate plant growth. Here, we investigate physiological incidence and mode of action pyoverdine from fluorescens C7R12 on Arabidopsis (Arabidopsis thaliana) plants grown under iron-sufficient or iron-deficient conditions. Pyoverdine was provided medium its iron-free structure...
Abstract Background Mitogen-activated protein kinases (MAPKs) are key regulators of immune responses in animals and plants. In Arabidopsis , perception microbe-associated molecular patterns (MAMPs) activates the MAPKs MPK3, MPK4 MPK6. Increasing information depicts events activated by MAMPs plants, but specific cooperative contributions these signalling largely unclear. Results this work, we analyse behaviour MPK3 MPK6 mutants early late triggered MAMP flg22 from bacterial flagellin. A...
Summary The analysis of the complex genome common wheat ( Triticum aestivum , 2 n = 6 x 42, formula AABBDD) is hampered by its large size (∼17 000 Mbp) and allohexaploid nature. In order to simplify analysis, we developed a generic strategy for dissecting such genomes into individual chromosomes. Chromosome 3B was successfully sorted flow cytometry cloned bacterial artificial chromosome (BAC), using only 1.8 million chromosomes an adapted protocol this purpose. BAC library (designated as...
FIT (FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR) is the central regulator of iron uptake in Arabidopsis thaliana roots. We performed transcriptome analyses six day-old seedlings and roots week-old plants using wild type, a fit knock-out mutant over-expression line grown under iron-sufficient or iron-deficient conditions. compared genes regulated FIT-dependent manner depending on developmental stage plants. assembled high likelihood dataset which we used to perform co-expression...
Iron is an essential growth determinant for plants, and plants acquire this micronutrient in amounts they need their environment. Plants can increase iron uptake response to a regulatory transcription factor cascade. Arabidopsis thaliana serves as model plant identify characterize regulation genes. Here, we show that overexpression of subgroup Ib bHLH bHLH039 (39Ox) caused constitutive acquisition responses, which resulted enhanced contents leaves seeds. Transcriptome analysis demonstrated...
Mitogen-activated protein kinases (MAPKs) are important regulators of plant immunity. Most the knowledge about function these pathways is derived from loss-of-function approaches. Using a gain-of-function approach, we investigated responses controlled by constitutively active (CA) MPK3 in Arabidopsis thaliana. CA-MPK3 plants dwarfed and display massive derepression defense genes associated with spontaneous cell death as well accumulation reactive oxygen species, phytoalexins, stress-related...
Abstract At low intensity, certain stress conditions may have a positive effect on growth and development of plants (eustress). Growth stimulation barley after gamma irradiation seeds in doses was observed as an increase root shoot lengths. Hydrogen peroxide concentrations shoots irradiated were significantly higher than control, while the opposite roots. To elucidate molecular basis seeds, we performed transcriptomic analysis embryos at different stages seed germination. The data correlated...
Abstract Flagellin perception is a keystone of pattern-triggered immunity in plants. The recognition this protein by plasma membrane (PM) receptor complex the beginning signaling cascade that includes phosphorylation and production reactive oxygen species (ROS). In both Arabidopsis (Arabidopsis thaliana) seedlings suspension cells, we found treatment with flg22, peptide corresponding to most conserved domain bacterial flagellin, caused rapid transient decrease level phosphatidylinositol (PI)...
Plant defense responses involve several biological processes that allow plants to fight against pathogenic attacks. How these different are orchestrated within organs and depend on specific cell types is poorly known. Here, using single-cell RNA sequencing (scRNA-seq) technology three independent replicates, we identified populations representing the core transcriptional of wild-type Arabidopsis leaves inoculated with bacterial pathogen Pseudomonas syringae DC3000. Among populations,...
The regulation of plant gene expression, necessary for development and adaptive responses, relies not only on RNA transcription but also messenger (mRNA) fate. To understand whether seed germination the degradation specific subsets mRNA, we investigated 5′ to 3′ decay machinery participated in this process. Arabidopsis (Arabidopsis thaliana) seeds exoribonuclease4 (xrn4) varicose (vcs) mutants displayed distinct dormancy phenotypes. Transcriptome analysis xrn4-5 vcs-8 mutant allowed us...
While it is generally acknowledged that drought one of the main abiotic factors affecting plant growth, how mineral nutrition specifically and negatively affected by water deficit has received very little attention, other than being analyzed as a consequence reduced growth. Therefore, Brassica napus plants were subjected to gradual onset deficits (mild, severe, or severe extended), leaves at ionomic, transcriptomic metabolic levels. The number Differentially Expressed Genes (DEGs) most...
Summary Common wheat ( Triticum aestivum L., 2 n = 6 x 42) is a polyploid species possessing one of the largest genomes among cultivated crops (1C approximately 17 000 Mb). The presence three homoeologous (A, B and D), prevalence repetitive DNA make sequencing genome daunting task. We have developed novel ‘chromosome arm‐based’ strategy for to simplify this task; relies on sub‐genomic libraries large inserts. In paper, we used di‐telosomic line isolate six million copies short arm chromosome...
Nodulation (Nod) factors (NFs) are symbiotic molecules produced by rhizobia that essential for establishment of the rhizobium–legume endosymbiosis. Purified NFs can stimulate lateral root formation (LRF) in Medicago truncatula, but little is known about molecular mechanisms involved. Using a combination reporter constructs, pharmacological and genetic approaches, we show act on early steps LRF M. independently ethylene signaling pathway cytokinin receptor MtCRE1, interaction with auxin. We...
Abstract Linking genotype and phenotype is a fundamental challenge in biology. In this respect, machine learning playing pivotal role systems As central phenotypic trait, fruit development its relative growth rate (RGR) result from interactions between gene regulation, metabolism environment. the present study, we carried out multispecies transcriptomic analysis of nine different fruits. To illustrate transcriptomes, transcripts were first compared using multivariate methods, revealing main...
Salmonella is one of the most prominent causes food poisoning and growing evidence indicates that contaminated fruits vegetables are an increasing concern for human health. Successful infection demands suppression host immune system, which often achieved via injection bacterial effector proteins into cells. In this report we present function protein in plant cell, supporting new concept trans-kingdom competence bacterium. We screened a range Typhimurium interference with immunity. Among...
Enhancers are key players in the spatio-temporal coordination of gene expression during numerous crucial processes, including tissue differentiation across development. Characterizing transcription factors (TFs) and genes they connect, molecular functions underpinned is important to better characterize developmental processes. In plants, recent characterization enhancers revealed their capacity activate several target genes. Nevertheless, identifying these at a genome-wide level challenging,...
Major nutritional and agronomical issues relating to maize (Zea mays) grains depend on the vitreousness/hardness of its endosperm. To identify corresponding molecular cellular mechanisms, most studies have been conducted opaque/floury mutants, recently Quality Protein Maize, a reversion an opaque2 mutation by modifier genes. These mutant lines are far from conventional crops. Therefore, dent flint inbred line were chosen for analysis transcriptome, amino acid, sugar metabolites developing...