A5050724409- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Plant Reproductive Biology
- Plant Parasitism and Resistance
- Plant-Microbe Interactions and Immunity
- Polysaccharides and Plant Cell Walls
- Legume Nitrogen Fixing Symbiosis
- Plant responses to water stress
- Plant and animal studies
- Plant tissue culture and regeneration
- Plant Pathogenic Bacteria Studies
- Plant Micronutrient Interactions and Effects
- Plant Growth Enhancement Techniques
- Light effects on plants
- Plant Genetic and Mutation Studies
- Crop Yield and Soil Fertility
- Plant Physiology and Cultivation Studies
- Microbial Community Ecology and Physiology
- Protist diversity and phylogeny
- Agronomic Practices and Intercropping Systems
- Genomics and Phylogenetic Studies
- Composting and Vermicomposting Techniques
- Seed Germination and Physiology
Institut Jean-Pierre Bourgin
2015-2024
AgroParisTech
2014-2024
Université Paris-Saclay
2017-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2017-2024
Centre National de la Recherche Scientifique
2015-2022
Génétique Quantitative et Évolution Le Moulon
2022
Département Santé des Plantes et Environnement
2017
Plant Biotechnology Institute
2015
Pyoverdines are siderophores synthesized by fluorescent Pseudomonas spp. Under iron-limiting conditions, these high-affinity ferric iron chelators excreted bacteria in the soil to acquire iron. produced beneficial ameliorate plant growth. Here, we investigate physiological incidence and mode of action pyoverdine from fluorescens C7R12 on Arabidopsis (Arabidopsis thaliana) plants grown under iron-sufficient or iron-deficient conditions. Pyoverdine was provided medium its iron-free structure...
Apical dominance occurs when the growing shoot tip inhibits outgrowth of axillary buds. Apically-derived auxin in nodal stem indirectly bud via cytokinins and strigolactones. Recently, sugar deprivation was found to contribute this phenomenon. Using rose pea, we investigated whether availability interacts with control, role strigolactones, vitro planta. We show that sucrose antagonises auxin's effect on outgrowth, a dose-dependent coupled manner. Sucrose also suppresses strigolactone...
Polycomb repressive complexes (PRCs) have been traditionally associated with the regulation of developmental processes in various organisms, including higher plants. However, similar to other epigenetic regulators, there is accumulating evidence for their role stress and immune-related pathways. In current study we show that PRC1 protein LHP1 required repression MYC2 branch jasmonic acid (JA)/ethylene (ET) pathway immunity. Loss induces reduction H3K27me3 levels gene bodies ANAC019 ANAC055,...
Abstract The modification of histones by acetyl groups has a key role in the regulation chromatin structure and transcription. Arabidopsis thaliana histone acetyltransferase GCN5 regulates modifications as part Spt-Ada-Gcn5 Acetyltransferase (SAGA) transcriptional coactivator complex. was previously shown to acetylate lysine 14 3 (H3K14ac) promoter regions its target genes even though binding did not systematically correlate with gene activation. Here, we explored mechanism through which...
Trees are long-lived organisms that continuously adapt to their environments, a process in which epigenetic mechanisms likely play key role. Via downregulation of the chromatin remodeler DECREASED IN DNA METHYLATION 1 (DDM1) poplar (Populus tremula × Populus alba) RNAi lines, we examined how methylation coordinates genomic and physiological responses moderate water deficit. We compared growth drought response two RNAi-ddm1 lines wild-type (WT) trees under well-watered deficit/rewatering...
In the model plant Arabidopsis (Arabidopsis thaliana), endogenous and environmental signals acting on shoot apical meristem cause acquisition of inflorescence fate. This results in changed patterns aerial development seen as transition from making leaves to production flowers separated by elongated internodes. Two related BEL1-like homeobox genes, PENNYWISE (PNY) POUND-FOOLISH (PNF), fulfill this transition. Loss function these genes impairs stem cell maintenance blocks internode elongation...
Glutamine synthetase (GS) is central for ammonium assimilation and consists of cytosolic (GS1) chloroplastic (GS2) isoenzymes. During plant ageing, GS2 protein decreases due to chloroplast degradation, GS1 activity increases support glutamine biosynthesis N remobilization from senescing leaves. The role the different Arabidopsis isoforms in nitrogen was examined using 15N tracing experiments. Only gln1;1-gln1;2-gln1;3 triple-mutation affecting three GLN1;1, GLN1;2, GLN1;3 genes significantly...
Despite a general view that asparagine synthetase generates as an amino acid for long-distance transport of nitrogen to sink organs, its role in metabolic pathways floral organs during seed filling has remained undefined. We demonstrate the onset pollination Arabidopsis induces selected genes metabolism, namely ASN1 (At3g47340), GLN2 (At5g35630), GLU1 (At5g04140), AapAT2 (At5g19950), ASPGA1 (At5g08100) and ASPGB1 (At3g16150), particularly at ovule stage (stage 0), accompanied by enhanced...
Strigolactones (SLs) are well known for their role in repressing shoot branching. In pea, increased transcript levels of SL biosynthesis genes observed stems highly branched deficient (ramosus1 (rms1) and rms5) response (rms3 rms4) mutants indicative negative feedback control. contrast, the rms2 mutant has reduced genes. Grafting studies hormone quantification led to a model where RMS2 mediates shoot-to-root signal that regulates both gene xylem sap cytokinin exported from roots. Here we...
Summary DWARF53 (D53) in rice ( Oryza sativa ) and its homologs Arabidopsis thaliana ), SUPPRESSOR OF MAX2‐LIKE 6 (SMXL6), SMXL7 SMXL8, are well established negative regulators of strigolactone (SL) signalling shoot branching regulation. Little is known pea Pisum sativum whether D53 related SMXLs specific to SL pathways. Here, we identify two allelic mutants, dormant3 dor3 demonstrate through gene mapping sequencing that DOR3 corresponds a homolog SMXL6/SMXL7, designated PsSMXL7. Phenotype...
Guanosine pentaphosphate and tetraphosphate (together referred to as ppGpp) are hyperphosphorylated nucleotides found in bacteria the chloroplasts of plants algae. In algae artificial ppGpp accumulation can inhibit chloroplast gene expression, influence photosynthesis, nutrient remobilization, growth, immunity. However, it is so far unknown whether required for abiotic stress acclimation plants. Here, we demonstrate that biosynthesis necessary nitrogen starvation
Chloroplasts can act as key players in the perception and acclimatization of plants to incoming environmental signals. A growing body evidence indicates that chloroplasts play a critical role plant immunity. Chloroplast function be regulated by nucleotides guanosine tetraphosphate pentaphosphate [(p)ppGpp]. In plants, (p)ppGpp levels increase response abiotic stress hormones which are involved biotic signalling. this study, we analysed transcriptome Arabidopsis over-accumulate (p)ppGpp,...
Bud outgrowth is a key process in the elaboration of yield and visual quality rose crops. Although light intensity well known to affect bud outgrowth, little on mechanisms involved this regulation. The objective work was test if control pattern along stem by photosynthetic photon flux density (PPFD) mediated sugars, cytokinins and/or abscisic acid intact plants. Rooted cuttings Rosa hybrida 'Radrazz' were grown growth chambers under high PPFD (520 µmol.m−2.s−1) until floral visible stage....
Symbiotic interactions between legume plants and rhizobia result in the formation of nitrogen-fixing nodules, but molecular actors mechanisms allowing for maintenance nodule identity are poorly understood. Medicago truncatula NODULE ROOT1 (MtNOOT1), Pisum sativum COCHLEATA1 (PsCOCH1), Lotus japonicus NOOT-BOP-COCH-LIKE1 (LjNBCL1) orthologs Arabidopsis (Arabidopsis thaliana) AtBLADE-ON-PETIOLE1/2 members NBCL gene family, which has conserved roles plant development is essential indeterminate...
Abstract Background Somatic embryogenesis (SE) exemplifies the unique developmental plasticity of plant cells. The regulatory processes, including epigenetic modifications controlling embryogenic reprogramming cell transcriptome, have just started to be revealed. Results To identify genes histone acetylation-regulated expression in SE, we analyzed global transcriptomes Arabidopsis explants undergoing induction response treatment with deacetylase inhibitor, trichostatin A (TSA). TSA-induced...
The shape and, therefore, the architecture of plant are dependent on genetic and environmental factors such as water supply. determines visual quality, a key criterion underlying decision to purchase an ornamental potted plant. aim this study was analyze genotypic responses eight rose bush cultivars alternation restriction re-watering periods, with soil potential -20kPa -10 kPa respectively. Responses were evaluated at architectural level through 3D digitalization using six variables...
Abstract Programmed cell death (PCD) is essential for several aspects of plant life. We previously identified the mips1 mutant Arabidopsis thaliana , which deficient enzyme catalysing myo-inositol synthesis, and that displays light-dependent formation lesions on leaves due to Salicylic Acid (SA) over-accumulation. Rationale this work was identify novel regulators PCD using a genetic approach. A screen secondary mutations abolish phenotype mutation in BIG gene, encoding factor unknown...
SUMMARY The secretory pathway is essential for plant immunity, delivering diverse antimicrobial molecules into the extracellular space. Arabidopsis thaliana soluble N‐ethylmaleimide‐sensitive‐factor attachment protein receptor SNAP33 a key actor of this process. snap33 mutant displays dwarfism and necrotic lesions, however molecular determinants its macroscopic phenotypes remain elusive. Here, we isolated several new mutants that exhibited constitutive cell death H 2 O accumulation, further...
SUMMARY Hydathodes are small organs found on the leaf margins of vascular plants which release excess xylem sap through a process called guttation. While previous studies have hinted at additional functions hydathode in metabolite transport or auxin metabolism, experimental support is limited. We conducted comprehensive transcriptomic, metabolomic and physiological analyses mature Arabidopsis hydathodes. This study identified 1460 genes differentially expressed hydathodes compared to blades,...
To complement N2 fixation through symbiosis, legumes can efficiently acquire soil mineral N adapted root architecture. However, architecture adaptation to availability has been little studied in legumes. Therefore, this study investigated the effect of nitrate on Medicago truncatula and assessed N-uptake potential a new highly branched mutant, TR185. The effects varying supply both uptake were characterized mutant wild type. Surprisingly, was not modified by variation supply. Moreover,...
A valuable strategy to improve crop yield consists in the use of plant growth-promoting rhizobacteria (PGPRs). However, influence PGPR colonization on physiology is largely unknown. Burkholderia phytofirmans strain PsJN (Bp PsJN) colonized only Arabidopsis thaliana roots after seed or soil inoculation. Foliar bacteria were detected leaf infiltration. Since, different bacterial times presence and/or locations host could lead physiological responses, photosynthesis, and metabolite profiles A....