A5048904242- Plant Molecular Biology Research
- Plant tissue culture and regeneration
- Plant Reproductive Biology
- Advances in Cucurbitaceae Research
- Seed and Plant Biochemistry
- Chromosomal and Genetic Variations
- Plant Stress Responses and Tolerance
- Plant-Microbe Interactions and Immunity
- Plant Genetic and Mutation Studies
- Transgenic Plants and Applications
- Plant nutrient uptake and metabolism
University of Silesia in Katowice
2018-2024
Auxin is an important regulator of plant ontogenies including embryo development and the exogenous application this phytohormone has been found to be necessary for induction embryogenic response in explants that have cultured vitro. However, present study, we show treatment Arabidopsis with trichostatin A (TSA), which a chemical inhibitor histone deacetylases, induces somatic embryogenesis (SE) without auxin. We TSA-treated generated embryos developed efficiently on adaxial side cotyledons,...
In plants, the embryogenic transition of somatic cells requires reprogramming cell transcriptome, which is under control genetic and epigenetic factors. Correspondingly, extensive modulation genes encoding transcription factors miRNAs has been indicated as controlling induction embryogenesis in Arabidopsis other plants. Among
Somatic embryogenesis (SE), which is a process that involves the in vitro-induced embryogenic reprogramming of plant somatic cells, requires dynamic changes cell transcriptome. These are fine-tuned by many genetic and epigenetic factors, including posttranslational histone modifications such as acetylation. Antagonistically acting enzymes, acetyltransferases (HATs) deacetylases (HDACs), control acetylation developmental processes, believed to SE. However, function specific HAT/HDACs genes...
Abstract Background Somatic embryogenesis (SE) exemplifies the unique developmental plasticity of plant cells. The regulatory processes, including epigenetic modifications controlling embryogenic reprogramming cell transcriptome, have just started to be revealed. Results To identify genes histone acetylation-regulated expression in SE, we analyzed global transcriptomes Arabidopsis explants undergoing induction response treatment with deacetylase inhibitor, trichostatin A (TSA). TSA-induced...
The embryogenic transition of somatic cells requires an extensive reprogramming the cell transcriptome. Relevantly, modulation genes that have a regulatory function, in particular encoding transcription factors (TFs) and miRNAs, been indicated as controlling embryogenesis (SE) is induced vitro plants. Identifying relationships between TFs miRNAs during SE induction central importance for understanding complex interplay fine-tunes transcriptome transition. Hence, here, we analysed AGL15...
Abstract Genotype-limited plant regeneration is one of the main obstacles to broader use genetic transformation in barley breeding. Thus, developing new approaches that might improve responses vitro recalcitrant genotypes remains at center biotechnology. Here, we analyzed different genotypes, including “Golden Promise,” a genotype commonly used transformation, and four malting cultivars poor regenerative potential. The expression hormone-related transcription factor (TF) genes with...
Epigenetic changes accompany the dynamic in cell wall composition during development of callus cells. H3K4me3 is responsible for active gene expression and reaction to environmental cues. Chromatin immunoprecipitation (ChIP) a powerful technique studying interplay between epigenetic modifications DNA regions interest. In combination with sequencing, it can provide genome-wide enrichment specific mark, providing vital information on its involvement plethora cellular processes. Here, we...