A5021844674- Plant Molecular Biology Research
- Bioenergy crop production and management
- Plant tissue culture and regeneration
- Plant responses to water stress
- Plant nutrient uptake and metabolism
- Plant Gene Expression Analysis
- Agriculture and Rural Development Research
- Immune Response and Inflammation
- Genetic Mapping and Diversity in Plants and Animals
- Plant Water Relations and Carbon Dynamics
- Digestive system and related health
- Historical Medical Research and Treatments
- Immunotherapy and Immune Responses
- Horticultural and Viticultural Research
- Epigenetics and DNA Methylation
- Natural Compound Pharmacology Studies
- Tree-ring climate responses
- Geological and Geophysical Studies Worldwide
- Collagen: Extraction and Characterization
- Neurology and Historical Studies
- Seed Germination and Physiology
- Genomics and Phylogenetic Studies
- Tree Root and Stability Studies
- Historical and Scientific Studies
- Monoclonal and Polyclonal Antibodies Research
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2024
Université d'Orléans
2013-2024
UMR BIOdiversity, GEnes & Communities
2024
Centre d'Études Scientifiques et Techniques d'Aquitaine
2024
Direction de la Recherche Fondamentale
2024
Centre National de Recherche en Génomique Humaine
2024
CEA Paris-Saclay
2024
Université Paris-Saclay
2024
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2024
Université Paris Cité
2024
Trees are long-lived organisms that continuously adapt to their environments, a process in which epigenetic mechanisms likely play key role. Via downregulation of the chromatin remodeler DECREASED IN DNA METHYLATION 1 (DDM1) poplar (Populus tremula × Populus alba) RNAi lines, we examined how methylation coordinates genomic and physiological responses moderate water deficit. We compared growth drought response two RNAi-ddm1 lines wild-type (WT) trees under well-watered deficit/rewatering...
A global DNA methylation and proteomics approach was used to investigate somatic embryo maturation in hybrid larch. Each developmental step during embryogenesis associated with a distinct significantly different level: from 45.8% mC for undifferentiated embryos (1-week proliferation) 61.5% immature maturation), while decrease 53.4% mature cotyledonary (8-weeks maturation). The presence of 5-azacytidine (hypo-methylating agent) or hydroxyurea (hyper-methylating the medium altered status...
The adaptive capacity of long-lived organisms such as trees to the predicted climate changes, including severe and successive drought episodes, will depend on presence genetic diversity phenotypic plasticity. Here, involvement epigenetic mechanisms in plasticity toward soil water availability was examined Populus×euramericana. This work aimed at characterizing (i) transcriptome plasticity, (ii) genome-wide DNA methylation, (iii) function genes affected by a drought–rewatering cycle shoot...
Trees have a long lifespan and must continually adapt to environmental pressures, notably in the context of climate change. Epigenetic mechanisms are doubtless involved phenotypic plasticity stress memory; however, little evidence role epigenetic processes is available for trees growing fields. Here, we analyzed possible involvement winter-dormant shoot apical meristem Populus × euramericana clones memory conditions faced during vegetative period. We aimed estimate range genetic...
Ectomycorrhizas are an intrinsic component of tree nutrition and responses to environmental variations. How epigenetic mechanisms might regulate these mutualistic interactions is unknown. By manipulating the level expression chromatin remodeler DECREASE IN DNA METHYLATION 1 (DDM1) two demethylases DEMETER-LIKE (DML) in Populus tremula × alba lines, we examined how host methylation modulates multiple parameters root colonization with fungus Laccaria bicolor. We compared ectomycorrhizas formed...
The effects of polysaccharide elicitors such as chitin, pectin, and dextran on the production phenylpropanoids (phenolics flavonoids) naphtodianthrones (hypericin pseudohypericin) in Hypericum perforatum shoot cultures were studied. Nonenzymatic antioxidant properties (NEAOP) peroxidase (POD) activity also observed extracts. activities phenylalanine ammonia lyase (PAL) chalcone-flavanone isomerase (CHFI) monitored to estimate channeling phenylpropanoid/flavonoid pathways elicited cultures. A...
Summary DNA methylation is involved in the control of plant development and adaptation to environment through modifications chromatin compaction gene expression. In poplar ( P opulus trichocarpa ), a perennial plant, variations have been reported between genotypes tissues or response drought. Nevertheless, relationships gene‐body methylation, expression still need clarification. Here, was mapped noncondensed fraction from . shoot apical meristematic cells, center morphogenesis, where could...
In sugar beet (Beta vulgaris altissima), bolting tolerance is an essential agronomic trait reflecting the response of genotypes after vernalization. Genes involved in induction have now been identified, and evidence suggests that epigenetic factors are their control. Indeed, time course amplitude DNA methylation variations shoot apical meristem shown to be critical inducing bolting, a few functional targets during vernalization identified. However, molecular mechanisms controlling levels...
Catharanthus roseus produces a wide spectrum of monoterpene indole alkaloids (MIAs). MIA biosynthesis requires tightly coordinated pathway involving more than 30 enzymatic steps that are spatio-temporally and environmentally regulated so some MIAs specifically accumulate in restricted plant parts. The first regulatory layer involves complex network transcription factors from the basic Helix Loop (bHLH) or AP2 families. In present manuscript, we investigated whether an additional epigenetic...
An epigenetic control of vernalization has been demonstrated in annual plants such as Arabidopsis and cereals, but the situation remains unclear biennial sugar beet that an absolute requirement for vernalization. The role DNA methylation flowering induction identification corresponding target loci also need to be clarified. In this context, (Beta vulgaris altissima) genotypes differing bolting tolerance were submitted various conditions different temperatures and/or methylating drugs....
Ongoing global changes affect ecosystems and open up new opportunities for biological invasion. The ability of invasive species to rapidly adapt environments represents a relevant model studying short-term adaptation mechanisms. aquatic plant, Ludwigia grandiflora subsp. hexapetala, is classified as harmful in European rivers. In French wet meadows, this has shown rapid transition from terrestrial with emergence two distinct morphotypes 5 years. To understand the heritable mechanisms...
Abstract In the last 20 years, several techniques have been developed for quantifying DNA methylation, most studied epigenetic marks in eukaryotes, including gold standard method, whole-genome bisulfite sequencing (WGBS). WGBS quantifies genome-wide methylation but has inconveniences rendering it less suitable population-scale studies. The high cost of deep and large amounts data generated prompted us to seek an alternative approach. Restricting studies parts genome would be a satisfactory...
The occurrence of proteins able to oxidize polyphenols even in the absence H2O2 was recently reported mung bean (Vigna radiata L.) hypocotyl cell wall extracts (R. Goldberg, A. Chabanet, A.M. Catesson [1993] In K.G. Welinder, S.K. Rasmussen, C. Penel, H. Greppin, eds, Plant Peroxidases: Biochemistry and Physiology, pp. 296-300). Therefore, possible presence a laccase investigated using immunocytological biochemical approaches. An enzyme catalyzing phenol oxidation molecular O2 extracted...
Sugar beet (Beta vulgaris altissima) is a biennial root crop with an absolute requirement for cold exposure to bolt and flower, process called vernalization. Global DNA methylation variations have been reported during vernalization in several plants. However, few genes targeted by described. The objectives of this study were identify differentially methylated regions their involvement bolting induction tolerance. Restriction landmark genome scanning was applied from shoot apical meristems...
In the context of climate changes, clarifying causes underlying tree phenotypic plasticity and adaptation is crucial. Studies suggest a role epigenetic mechanisms in response to external stimuli, raising question whether such processes can promote acclimation trees exposed adverse conditions. Recently, we revealed an environmental footprint shoot apical meristem (SAM) which could partially be transmitted mitotically, for several months, up until winter-dormant bud field Here, extended our...
During differentiation, in vitro organogenesis calls for the adjustment of gene expression program toward a new fate. The role epigenetic mechanisms including DNA methylation is suggested but little known about loci affected by changes, particularly agronomic plants witch technologies are useful such as sugar beet. Here, three pairs organogenic and non‐organogenic cell lines originating from different beet ( Beta vulgaris altissima ) cultivars were used to assess dynamics at global or genic...