A5071537878- Epigenetics and DNA Methylation
- Cancer Genomics and Diagnostics
- RNA modifications and cancer
- Renal cell carcinoma treatment
- Genetic Syndromes and Imprinting
- Birth, Development, and Health
- Cancer-related gene regulation
- Renal and related cancers
- Genomics and Chromatin Dynamics
- Cancer-related molecular mechanisms research
- Asthma and respiratory diseases
- Health, Environment, Cognitive Aging
- Prenatal Screening and Diagnostics
- Glutathione Transferases and Polymorphisms
- Immune Cell Function and Interaction
- MicroRNA in disease regulation
- IL-33, ST2, and ILC Pathways
- Nutrition, Genetics, and Disease
- Plant Molecular Biology Research
- Neonatal Respiratory Health Research
- Pregnancy and preeclampsia studies
- Genomics and Phylogenetic Studies
- Diabetes and associated disorders
- Air Quality and Health Impacts
- CRISPR and Genetic Engineering
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2016-2025
Université Paris-Saclay
2021-2025
Centre National de Recherche en Génomique Humaine
2016-2025
Direction de la Recherche Fondamentale
2017-2025
CEA Paris-Saclay
2021-2025
CEA Paris-Saclay - Etablissement de Fontenay-aux-roses
2019-2024
Assistance Publique – Hôpitaux de Paris
2009-2024
Centre National de la Recherche Scientifique
2006-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2024
Institut Agro Montpellier
2024
Oilseed rape (Brassica napus L.) was formed ~7500 years ago by hybridization between B. rapa and oleracea, followed chromosome doubling, a process known as allopolyploidy. Together with more ancient polyploidizations, this conferred an aggregate 72× genome multiplication since the origin of angiosperms high gene content. We examined consequences its recent duplication. The constituent An Cn subgenomes are engaged in subtle structural, functional, epigenetic cross-talk, abundant homeologous...
Background: Huge progress has been made in the development of array- or sequencing-based technologies for DNA methylation analysis. The Illumina Infinium® Human Methylation 450K BeadChip (Illumina Inc., CA, USA) allows simultaneous quantitative monitoring more than 480,000 CpG positions, enabling large-scale epigenotyping studies. However, assay combines two different chemistries, which may cause a bias analysis if all signals are merged as unique source measurement. Materials & methods: We...
The Human Epigenome Project aims to identify, catalogue, and interpret genome-wide DNA methylation phenomena. Occurring naturally on cytosine bases at cytosine–guanine dinucleotides, is intimately involved in diverse biological processes the aetiology of many diseases. Differentially methylated cytosines give rise distinct profiles, thought be specific for gene activity, tissue type, disease state. identification such variable positions will significantly improve our understanding genome...
Monozygotic (MZ) twin pair discordance for childhood-onset Type 1 Diabetes (T1D) is ∼50%, implicating roles genetic and non-genetic factors in the aetiology of this complex autoimmune disease. Although significant progress has been made elucidating genetics T1D recent years, component remained poorly defined. We hypothesized that epigenetic variation could underlie some and, thus, performed an epigenome-wide association study (EWAS) generated genome-wide DNA methylation profiles purified...
DNA methylation patterns are altered in numerous diseases and often correlate with clinically relevant information such as disease subtypes, prognosis drug response. With suitable assays after validation large cohorts, associations can be exploited for clinical diagnostics personalized treatment decisions. Here we describe the results of a community-wide benchmarking study comparing performance all widely used methods analysis that compatible routine use. We shipped 32 reference samples to...
Significance This work shows that active DNA demethylation governs ripening, an important plant developmental process. Our defines a molecular mechanism, which has until now been missing, to explain the correlation between genomic and fruit ripening. It demonstrates direct cause-and-effect relationship induction of gene expression in fruits. The importance these findings goes far beyond understanding biology ripening provides innovative strategy for its fine control through modulation...
Abstract The global increase in lung cancer burden, together with its poor survival and resistance to classical chemotherapy, underscores the need for identification of critical molecular events involved carcinogenesis. Here, we have applied quantitative profiling DNA methylation states a panel five cancer-associated genes (CDH1, CDKN2A, GSTP1, MTHFR, RASSF1A) large case-control study cancer. Our analyses revealed high frequency aberrant hypermethylation RASSF1A, CDKN2A tumors as compared...
Background Changes in imprinted gene dosage the placenta may compromise prenatal control of nutritional resources. Indeed monoallelic behaviour and sensitivity to changes regional epigenetic state render genes both vulnerable adaptable. Methods Findings We investigated whether a high-fat diet (HFD) during pregnancy modified expression local global DNA methylation patterns placenta. Pregnant mice were fed HFD or (CD) first 15 days gestation. compared total homogenates, for male female...
The O(6)-methylguanine-DNA methyltransferase gene (MGMT) is methylated in several cancers, including gliomas. However, the functional role of cysteine-phosphate-guanine (CpG) island (CGI) methylation MGMT silencing still controversial. aim this study was to investigate whether CGI correlates inversely with RNA expression glioblastomas and determine CpG region whose best reflects level expression. sites that are potentially related investigated 54 by pyrosequencing, a highly quantitative...
Abstract Background Ductal carcinoma in situ (DCIS) of the breast is a precursor invasive carcinoma. DNA methylation alterations are thought to be an early event progression cancer, and may prove valuable as tool clinical decision making for understanding neoplastic development. Results We generate genome-wide profiles 285 tissue samples representing validate changes between normal DCIS independent dataset 15 40 samples. also prognostic signature on 583 cancer from The Cancer Genome Atlas....
Abstract Background Genetic susceptibility and environmental influences are important contributors to the development of asthma atopic diseases. Epigenetic mechanisms may facilitate gene by environment interactions in these Methods We studied rural birth cohort PASTURE (Protection against allergy: study environments) investigate (a) whether epigenetic patterns candidate genes influenced farm exposure general, (b) change over first years life, (c) changes contribute asthma. DNA was extracted...