A5056661622- Advanced biosensing and bioanalysis techniques
- Nitric Oxide and Endothelin Effects
- RNA Interference and Gene Delivery
- RNA and protein synthesis mechanisms
- MicroRNA in disease regulation
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Plant Molecular Biology Research
- CRISPR and Genetic Engineering
- Hemoglobin structure and function
- Natural product bioactivities and synthesis
- Circular RNAs in diseases
- Metal-Catalyzed Oxygenation Mechanisms
- Protein Kinase Regulation and GTPase Signaling
- Immunotherapy and Immune Responses
- Radiomics and Machine Learning in Medical Imaging
- Hepatocellular Carcinoma Treatment and Prognosis
- Endoplasmic Reticulum Stress and Disease
- Bioactive natural compounds
- Plant tissue culture and regeneration
- Biological Activity of Diterpenoids and Biflavonoids
- Immune Response and Inflammation
- Autophagy in Disease and Therapy
- Virus-based gene therapy research
- Ubiquitin and proteasome pathways
Sun Yat-sen University
2022-2025
Fifth Affiliated Hospital of Sun Yat-sen University
2022-2025
Anhui Medical University
2025
Ningbo Medical Center Lihuili Hospital
2020-2024
Ningbo University
2024
Cleveland Clinic
1994-2023
Kashi University
2023
Zhejiang University
2021-2022
Wuhan University of Technology
2022
Shijiazhuang University
2022
Significance This work shows that active DNA demethylation governs ripening, an important plant developmental process. Our defines a molecular mechanism, which has until now been missing, to explain the correlation between genomic and fruit ripening. It demonstrates direct cause-and-effect relationship induction of gene expression in fruits. The importance these findings goes far beyond understanding biology ripening provides innovative strategy for its fine control through modulation...
The nitric oxide synthase oxygenase domain (NOS ox ) oxidizes arginine to synthesize the cellular signal and defensive cytotoxin (NO). Crystal structures determined for cytokine-inducible NOS reveal an unusual fold heme environment stabilization of activated oxygen intermediates key catalysis. A winged β sheet engenders a curved α-β resembling baseball catcher's mitt with clasped in palm. location exposed hydrophobic residues results mutational analysis place dimer interface adjacent...
The molecular machinery required for autophagy is highly conserved in all eukaryotes as seen by the high degree of conservation proteins involved formation autophagosome membranes. Recently, both yeast Apg8p and its rat homologue Map1lc3 were identified essential constituents membrane a processed form. In addition, human exist two modified forms produced series post-translational modifications including critical C-terminal cleavage after Gly residue, smaller form associated with Herein, we...
Incomplete radiofrequency ablation (IRFA) triggers mild protective autophagy in residual tumor cells and results an immunosuppressive microenvironment. This accelerates the recurrence of tumors causes resistance to anti-PD-1/PDL1 therapy, which bringing a great clinical challenge immunotherapy. Mild activation can promote cancer cell survival while further amplification contributes immunogenic death (ICD). To this regard, we constructed active targeting zeolitic imidazolate framework-8...
The oxygenase domain of inducible NO synthase (residues 1−498, iNOSox) is the enzyme's catalytic center. Its active form a homodimer that contains heme and tetrahydrobiopterin (H4biopterin) binds l-arginine [Ghosh, D. K., & Stuehr, J. (1995) Biochemistry 34, 801]. To help identify protein residues involved in prosthetic group dimeric interaction, we expressed H4biopterin-free iNOSox Escherichia coli. was 80% but contained low-spin iron bound DTT as sixth ligand. H4biopterin or with high...
Endothelial nitric-oxide synthase (eNOS) is targeted to caveoli through interaction with caveolin-1 (cav-1). cav-1 binding a consensus site in the eNOS oxygenase domain proposed antagonize calmodulin (CaM) and thereby inhibit nitric oxide (NO) synthesis. To study mechanism, we examined how scaffolding peptide (amino acids 82–101; cav-1P) would affect NO synthesis, NADPH oxidation, cytochrome <i>c</i> reduction, ferricyanide reduction by full-length or its isolated reductase domains. Cav-1P...
ITP and dITP exist in all cells. is potentially mutagenic, the levels of these nucleotides are controlled by inosine triphosphate pyrophosphatase (EC3.6.1.19). Here we report cloning, expression, characterization a 21.5-kDa human (hITPase), an enzyme whose activity has been reported many animal tissues studied populations but protein sequence not determined before. At optimal pH 10.0, recombinant hITPase hydrolyzed ITP, dITP, xanthosine 5′-triphosphate to their respective monophosphates...
In plants, microRNAs (miRNAs) play essential roles in growth, development, yield, stress response and interactions with pathogens. However no miRNA has been experimentally documented to be functionally involved fruit ripening although many miRNAs have profiled fruits. Here we show that SlymiR157 SlymiR156 differentially modulate softening tomato (Solanum lycopersicum). is expressed developmentally regulated normal fruits those of the Colourless non-ripening (Cnr) epimutant. It regulates...
Construction of synthetic circuits that can reprogram genetic networks and signal pathways is a long-term goal for manipulation biosystems. However, it still highly challenging to build artificial communications among endogenous RNA species due their sequence independence structural diversities. Here we report an RNA-based circuit establish regulatory linkages between expression genes in both Escherichiacoli mammalian cells. This design employs displacement-assembly approach modulate the...
Abstract Naturally-occurring epimutants are rare and have mainly been described in plants. However how these mutants maintain their epigenetic marks they inherited remain unknown. Here we report that CHROMOMETHYLASE3 ( SlCMT3 ) other methyltransferases required for maintenance of a spontaneous epimutation its cognate Colourless non-ripening Cnr phenotype tomato. We screened series DNA methylation-related genes could rescue the hypermethylated mutant. Silencing developmentally-regulated gene...
The miRNAs are small, non-coding RNAs that regulate various biological processes, including liver fibrosis. Hepatic stellate cells (HSCs) play a central role in the pathogenesis of By microarray profiling and real-time PCR, we noted miR-31 expression HSCs from rats, mice humans was significantly increased during HSC activation culture. Overall, levels were unchanged whole-liver RNA extracts fibrotic rat human samples. Nevertheless, found particularly up-regulated but not hepatocytes...
The characteristics of tetrahydrobiopterin (H4biopterin) binding to pteridine-free recombinant macrophage inducible nitric oxide synthase expressed in Escherichia coli were investigated with a special focus given effects caused by 2,4-diamino-5,6,7, 8-tetrahydro-6-(l-erythro-1,2-dihydroxypropyl)pteridine (4-amino-H4biopterin), novel pterin-based inhibitor synthase. 4-amino compound completely inhibited enzyme stimulation 10 microM H4biopterin half-maximally active concentration 7.2 +/- 0.39...
Inducible nitric oxide (NO) synthase (iNOS) is comprised of an oxygenase domain containing heme, tetrahydrobiopterin, the substrate binding site, and a reductase FAD, FMN, calmodulin, NADPH site. Enzyme activity requires dimeric interaction between two domains with attached as monomeric extensions. To understand how dimerization activates iNOS, we synthesized iNOS heterodimer one full-length subunit histidine-tagged that was missing its domain. The purified using nickel-Sepharose 2′,5′-ADP...
Red light (RL) can stimulate plant defense against foliar diseases; however, its role in activation of systemic root diseases remains unclear. Here, the effect RL on knot nematode Meloidogyne incognita (RKN) infestation was investigated watermelon plants (Citrullus lanatus L.). Plants were exposed to 200 μmol m-2 s-1 photosynthetic photon flux density at canopy level for 21 days using light-emitting photodiodes. The results showed that significantly suppressed gall formation and development,...
Engineering of genetic networks with artificial signaling pathways (ASPs) can reprogram cellular responses and phenotypes under different circumstances for a variety diagnostic therapeutic purposes. However, construction ASPs between originally independent endogenous genes in mammalian cells is highly challenging. Here we report an amplifiable RNA circuit that theoretically build regulatory connections any cells. We harness the system catalytic hairpin assembly combination controllable...
RNA-cleaving ribozymes are promising candidates as general tools of RNA interference (RNAi) in gene manipulation. However, compared with other systems, such siRNA and CRISPR technologies, the ribozyme still far from broad applications on RNAi due to their poor performance cellular context. In this work, we report an efficient tool based chemically modified hammerhead (HHR). By introduction intramolecular linkage into minimal HHR reconstruct distal interaction within tertiary structure,...
The oxygenase domain of the mouse cytokine-inducible nitric-oxide synthase (iNOSox, amino acids 1−498) binds heme, tetrahydrobiopterin, and substrate Arg is responsible for catalyzing nitric oxide synthesis maintaining enzyme's active dimeric structure. To further understand iNOSox structure−function, we carried out alanine point mutagenesis on 15 conserved acidic residues located within a region (amino 352−473) that shares sequence homology with pterin-binding module in dihydrofolate...
Breast cancer metastasis suppressor 1 (BRMS1) was originally identified as an active in human breast cancer. Loss of BRMS1 expression correlates with tumor progression, and suppresses several steps required for metastasis. However, the role hepatocellular carcinoma (HCC) remains elusive. In this study, we found that level significantly down-regulated HCC tissues. Expression SK-Hep1 cells did not affect cell growth under normal culture conditions, but sensitized to apoptosis induced by serum...