A5031320592- Reproductive System and Pregnancy
- Pregnancy and preeclampsia studies
- Endometriosis Research and Treatment
- Birth, Development, and Health
- Liver Disease Diagnosis and Treatment
- Cholesterol and Lipid Metabolism
- Uterine Myomas and Treatments
- Diet, Metabolism, and Disease
- Immune responses and vaccinations
- Endometrial and Cervical Cancer Treatments
- Systemic Sclerosis and Related Diseases
- Lipid metabolism and disorders
- Sexual Differentiation and Disorders
- Biochemical and Molecular Research
- Lung Cancer Treatments and Mutations
- Microtubule and mitosis dynamics
- Cellular transport and secretion
- Mast cells and histamine
- Urticaria and Related Conditions
- PI3K/AKT/mTOR signaling in cancer
- Autophagy in Disease and Therapy
- Genetic Syndromes and Imprinting
- Fibroblast Growth Factor Research
- Lipid metabolism and biosynthesis
- IL-33, ST2, and ILC Pathways
Inserm
2014-2024
Université Paris Cité
2012-2024
Centre National de la Recherche Scientifique
2012-2024
Institut Cochin
2014-2024
Hôpital Cochin
2022
Assistance Publique – Hôpitaux de Paris
2020-2022
Beth Israel Deaconess Medical Center
2016-2022
Harvard University
2016-2022
Groupe Hospitalier Cochin - Port-Royal, Hôtel-Dieu, Broca - La Collégiale
2020
Délégation Paris 5
2012-2019
Obese, insulin-resistant states are characterized by a paradoxical pathogenic condition in which the liver appears to be selectively insulin resistant. Specifically, fails suppress glucose production, yet successfully stimulates de novo lipogenesis. The mechanisms underlying this dysregulation remain controversial. Here, we hypothesized that carbohydrate-responsive element-binding protein (ChREBP), transcriptional activator of glycolytic and lipogenic genes, plays central role paradox....
Increased fructose consumption is a contributor to the burgeoning epidemic of non-alcoholic fatty liver disease (NAFLD). Recent evidence indicates that metabolic hormone FGF21 regulated by in humans and rodents may play functional role this nutritional context. Here, we sought define mechanism which ingestion regulates determine whether contributes an adaptive response consumption. We tested transcription factor carbohydrate responsive-element binding protein (ChREBP) fructose-mediated...
Abstract Chronic inflammation and fibrosis can result from inappropriately activated immune responses that are mediated by macrophages. Macrophages acquire memory-like characteristics in response to antigen exposure. Here, we show the effect of BCG or low-dose LPS stimulation on macrophage phenotype, cytokine production, chromatin metabolic modifications. Low-dose training alleviates a mouse model systemic sclerosis (SSc), whereas BCG-training exacerbates disease this model. Adoptive...
Preeclampsia (PE) is a common human-specific pregnancy disorder defined by hypertension and proteinuria during gestation responsible for maternal fetal morbimortality. STOX1 , encoding transcription factor, was the first gene associated with PE as identified positional cloning approaches. Its overexpression in choriocarcinoma cells mimics transcriptional consequences of human placenta. Here, we created transgenic mouse strains overexpressing . Wild-type female mice crossed male reproduce...
Digit length ratios, especially the second-to-fourth digit ratio (2D : 4D), are associated with various pathological and behavioural conditions in many species including humans dependent upon prenatal androgen to oestrogen balance. It is unknown whether ratios modified by environmental exposure ubiquitous endocrine disruptors. We studied effect on adult male Wistar rat of a gestational oestrogenic antiandrogenic compounds bisphenol A (BPA), genistein vinclozolin, low doses, combination...
SERPINA3 (Serpin peptidase inhibitor clade A member 3), also known as a1-antichymotrypsin, is a serine protease involved in wide range of biological processes. Recently, it has been shown to be up-regulated human placental diseases association with hypomethylation the 5' region gene. In present study, we show that promoter transcriptionally activated by three transcription factors (TFs) (SP1, MZF1 and ZBTB7B), level induction being dependent on rs1884082 single nucleotide polymorphism (SNP)...
Increased sugar consumption is a risk factor for the metabolic syndrome including obesity, hypertriglyceridemia, insulin resistance, diabetes, and nonalcoholic fatty liver disease (NAFLD). Carbohydrate responsive element–binding protein (ChREBP) transcription that responds to regulate adaptive programs. Hepatic ChREBP particularly fructose global ChREBP-KO mice are intolerant diets containing fructose. It has recently been suggested protects from hepatotoxicity following high-fructose...
Storkhead box 1 (STOX1) is a winged-helix transcription factor that implicated in the genetic forms of high-prevalence human gestational disease, pre-eclampsia. STOX1 overexpression confers pre-eclampsia-like transcriptomic features to trophoblastic cell lines and pre-eclampsia symptoms pregnant mice. The aim this work was evaluate impact on free radical equilibrium mitochondrial function, both vitro vivo.Transcriptome analysis STOX1-transgenic versus nontransgenic placentas at 16.5 days...
Endometriosis is a frequent, chronic, inflammatory gynecological disease characterized by the presence of ectopic endometrial tissue causing pain and infertility. Macrophages have central role in lesion establishment maintenance driving chronic inflammation remodeling. can be reprogrammed to acquire memory-like characteristics after antigenic challenge reinforce or inhibit subsequent immune response, phenomenon termed "trained immunity." Here, whereas bacille Calmette-Guérin (BCG) training...
Preeclampsia (PE) is the major pregnancy-induced hypertensive disorder responsible for maternal and fetal morbidity mortality that can be associated with intrauterine growth restriction (IUGR). PE IUGR are thought to due a placental defect, occurring early during pregnancy. Several microRNAs (miRNAs) have been shown deregulated in context of diseases could thus play role pathophysiology PE. Here, we show pri-miR-34a overexpressed preeclamptic placentas its expression much higher first...
Abstract An anonymous online survey in French was used to assess if endometriosis patients would be as ready unaffected women donate their menstrual blood for biological research on and evaluate potential barriers such donation. It distributed September 2022 by social media two mailing lists, including a patient organization. The questionnaire assessed participant age brief medical history (hormonal contraception, diagnosis, type of endometriosis), experience (menstrual abundance,...
Abstract Preeclampsia is a disease of pregnancy involving systemic endothelial dysfunction. However, cardiovascular consequences preeclampsia are difficult to analyze in humans. The objective the present study evaluate dysfunction induced by examining endothelium mice suffering severe STOX1 overexpression. Using Next Generation Sequencing on cells carrying either transgenic or control embryos, we discovered significant alterations gene networks involved inflammation, cell cycle and cardiac...
Carbohydrate response element-binding protein (ChREBP) is a carbohydrate-sensing transcription factor that regulates both adaptive and maladaptive genomic responses in coordination of systemic fuel homeostasis. Genetic variants the ChREBP locus associate with diverse metabolic traits humans, including circulating lipids. To identify novel ChREBP-regulated hepatokines contribute to its effects, we integrated ChIP-Seq analysis mouse liver human genetic data for lipid identified hepatocyte...
Endometriosis is characterized by the presence of ectopic endometrial cells outside uterine cavity. Thyroid autoimmunity has been associated with endometriosis. This work investigated potential pathophysiological link between endometriosis and thyroid disorders. Transcripts proteins involved in metabolism are dysregulated eutopic endometrium endometriotic patients, leading to resistance triiodothyronine (T3) action local accumulation thyroxine (T4). Thyroid-stimulating hormone (TSH) acts as...
Abstract Preservation and expansion of β-cell mass is a therapeutic goal for diabetes. Here we show that the hyperactive isoform carbohydrate response-element binding protein (ChREBPβ) nuclear effector hyperglycemic stress occurring in β-cells response to prolonged glucose exposure, high-fat diet, We transient positive feedback induction ChREBPβ necessary adaptive metabolic challenges. Conversely, chronic excessive β-cell-specific overexpression results loss identity, apoptosis, mass,...
Abstract STUDY QUESTION What are the effects of B lymphocyte inactivation or depletion on progression endometriosis? SUMMARY ANSWER Skewing activated cells toward regulatory (Bregs) by Bruton’s tyrosine kinase (Btk) inhibition using Ibrutinib prevents endometriosis in mice while cell an anti-CD20 antibody has no effect. WHAT IS KNOWN ALREADY A polyclonal activation and presence anti-endometrial autoantibodies have been described a large proportion women with though their exact role disease...
Preeclampsia (PE) and vascular intra-uterine growth restriction (vIUGR) are two pathological obstetrical conditions originating from placental dysfunction. Recently, methylation changes at the level have been shown to be indicative of these diseases. The alteration such epigenetic marks is therefore a novel pathway that might critical for pathologies. Here, we identified region located in distal promoter T-box-containing transcription factor TBX15 differentially methylated placentas....
STOX1 is a transcription factor involved in preeclampsia and Alzheimer disease. We show that the knock-down of gene induces rather mild effect on expression trophoblast cell lines (BeWo). identified binding sites shared by two major isoforms, STOX1A STOX1B. Profiling cells overexpressing either or STOX1B, we genes downregulated both with site their promoters. Among those, STOX1-induced Annexin A1 downregulation led to abolished membrane repair BeWo cells. By contrast, overexpression B has...