A5062734360- Plant Gene Expression Analysis
- Polysaccharides and Plant Cell Walls
- Food composition and properties
- Lignin and Wood Chemistry
- Gene expression and cancer classification
- Horticultural and Viticultural Research
- Plant tissue culture and regeneration
- Plant pathogens and resistance mechanisms
- Fermentation and Sensory Analysis
- Imbalanced Data Classification Techniques
- Natural Fiber Reinforced Composites
- Biofuel production and bioconversion
- Rough Sets and Fuzzy Logic
- Anomaly Detection Techniques and Applications
- Data Mining Algorithms and Applications
- Botanical Research and Applications
- Seed Germination and Physiology
- Plant Molecular Biology Research
- Biomedical Text Mining and Ontologies
- Microbial Metabolites in Food Biotechnology
- Artificial Intelligence in Healthcare
- Computational Drug Discovery Methods
- Phytochemicals and Antioxidant Activities
- Biochemical and biochemical processes
- Adversarial Robustness in Machine Learning
Université de Lille
2015-2024
Centre National de la Recherche Scientifique
2003-2024
Unité de Glycobiologie Structurale et Fonctionnelle
2016-2024
University of Cambridge
2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2022-2023
Laboratoire de Recherche en Sciences Végétales
2005
Commonwealth Scientific and Industrial Research Organisation
1999-2004
Université d'Orléans
2003
Les Hôpitaux de Chartres
2003
Physiologie, Ecologie et Environnement
2003
We have proposed replicator neural networks (RNNs) for outlier detection. compare RNN detection with three other methods using both publicly available statistical datasets (generally small) and data mining much larger generally real data). The smaller provide insights into the relative strengths weaknesses of RNNs. in particular test scalability practicality application.
Summary One of the main characteristics plant cells is presence cell wall located outside plasma membrane. In particular cells, this can be reinforced by lignin, a polyphenolic polymer that plays central role for vascular plants, conferring hydrophobicity to conducting tissues and mechanical support upright growth. Lignin has been studied extensively range different techniques, including anatomical morphological analyses using dyes characterize localization in situ . With constant...
Bast fibres are characterized by very thick secondary cell walls containing high amounts of cellulose and low lignin contents in contrast to the heavily lignified typically found xylem tissues. To improve quality fiber-based products future, a thorough understanding main wall polymer biosynthetic pathways is required. In this study we have carried out characterization genes involved biosynthesis flax along with some their regulation mechanisms. We first identified members phenylpropanoid...
Abstract Reported herein is an in vivo triple labelling strategy to monitor the formation of plant cell walls. Based on a combination copper‐catalysed alkyne–azide cycloaddition (CuAAC), strain‐promoted azide–alkyne (SPAAC), and Diels–Alder reaction with inverse electronic demand (DAR inv ), this methodology can be applied various species interest research. It allowed detection differential incorporation alkynyl‐, azido‐, methylcyclopropenyl‐tagged reporters three main monolignols into de...
Monolignols are the building blocks for lignin polymerization in apoplastic domain. Monolignol biosynthesis, transport, storage, glycosylation, and deglycosylation main biological processes partaking their homeostasis. In Arabidopsis thaliana, members of uridine diphosphate-dependent glucosyltransferases UGT72E UGT72B subfamilies have been demonstrated to glycosylate monolignols. Here, poplar UGT72 family, which is clustered into four groups, was characterized: Group 1 UGT72AZ1 UGT72AZ2,...
Lignin is present in plant secondary cell walls and among the most abundant biological polymers on Earth. In this work we investigated potential role of UGT72E gene family regulating lignification Arabidopsis. Chemical determination floral stem lignin contents ugt72e1, ugt72e2, ugt72e3 mutants revealed no significant differences compared to WT plants. contrast, use a novel safranin O ratiometric imaging technique indicated increase wall content both interfascicular fibers xylem from young...
Summary To learn more about lignin formation in response to wounding trees, we adopted two complementary approaches: (1) microscopic and histochemical studies of the wound 3.5‐month‐old Eucalyptus gunnii plantlets (2) biochemical investigations hydroxycinnamyl alcohol dehydrogenase activities wounded 6‐year‐old, field‐grown E. trees. The first approach revealed that a barrier zone was formed both ground tissues (cortex pith reaction zone) vascular tissues. barely detectable after 24 h but...
In order to learn more about the diversity of genes expressed during flax fibre cell wall formation, sequence tags (ESTs) were obtained from a cDNA library derived outer fibre-bearing tissues (Linum usitatissimum) stems (cv Hermes) harvested at mid-flowering stage. After elimination vector and unreadable sequences, 927 ESTs grouped into 67 clusters 754 singletons. The have been submitted dbEST GenBank databases with accession numbers 25939634 - 25940560 (dbEST) CV478070 CV478996 (GenBank)....
Experimentally-generated (nanoLC-MS/MS) proteomic analyses of four different flax organs/tissues (inner-stem, outer-stem, leaves and roots) enriched in proteins from 3 sub-compartments (soluble-, membrane-, cell wall-proteins) was combined with publically available data on seed whole-stem to generate a protein database containing 2996 nonredundant total proteins. Subsequent multiple (MapMan, CAZy, WallProtDB expert curation) this were then used identify wall proteome consisting 456 localized...
This article describes a methodology for detailed mapping of the lignification capacity plant cell walls that we have called "REPRISAL" REPorter Ratiometrics Integrating Segmentation Analyzing Lignification. REPRISAL consists combination three separate approaches. In first approach, H*, G*, and S* monolignol chemical reporters, corresponding to p-coumaryl alcohol, coniferyl sinapyl are used label growing lignin polymer in fluorescent triple labeling strategy based on sequential use main...
Abstract Reported herein is an in vivo triple labelling strategy to monitor the formation of plant cell walls. Based on a combination copper‐catalysed alkyne–azide cycloaddition (CuAAC), strain‐promoted azide–alkyne (SPAAC), and Diels–Alder reaction with inverse electronic demand (DAR inv ), this methodology can be applied various species interest research. It allowed detection differential incorporation alkynyl‐, azido‐, methylcyclopropenyl‐tagged reporters three main monolignols into de...
Germins and germin-like proteins (GLPs) constitute a large highly diverse family of ubiquitous plant cell wall proteins. These seem to be involved in many developmental stages stress-related processes, but their exact participation these processes generally remains obscure. In Pinus caribaea Morelet, the PcGER1 gene is expressed uniquely embryo tissues, encodes GLP ionically bound walls pine cells maintained 2,4-D-containing medium. We have cloned genomic fragment including 1520 bp...
niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français étrangers, laboratoires publics privés.
One of the biggest challenges for a more widespread utilization plant fibers is to better understand different molecular factors underlying variability in fineness and mechanical properties both elementary scutched fibers. Accordingly, we analyzed genome-wide transcription profiling from bast fiber bearing tissues seven flax varieties (4 spring, 2 winter 1 linseed) identified 1041 differentially expressed genes between varieties, which 97 were related cell wall metabolism. KEGG analysis...