A5010520646- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Meat and Animal Product Quality
- Microbial Metabolites in Food Biotechnology
- Microbial Metabolic Engineering and Bioproduction
- Advanced Chemical Sensor Technologies
- Probiotics and Fermented Foods
- Enzyme Structure and Function
- Enzyme Production and Characterization
- Galectins and Cancer Biology
- Genomics and Phylogenetic Studies
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Amino Acid Enzymes and Metabolism
- Bacterial biofilms and quorum sensing
- Enzyme Catalysis and Immobilization
- Diet, Metabolism, and Disease
- Phytase and its Applications
- Antifungal resistance and susceptibility
- Identification and Quantification in Food
- Food composition and properties
- Microbial Community Ecology and Physiology
- Microbial Inactivation Methods
- Bacillus and Francisella bacterial research
- Microscopic Colitis
Centre National de la Recherche Scientifique
2011-2024
Université de Lille
2010-2024
Unité de Glycobiologie Structurale et Fonctionnelle
2013-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2023
Microbiologie de l’alimentation au service de la santé
2011
École Nationale Supérieure de Chimie de Lille
1999
Abstract BACKGROUND: A simple method based on solid phase microextraction/gas chromatography/mass spectrometry (SPME/GC/MS) was applied for studying the volatile profiles of whole fish samples European sea bass ( Dicentrarchus labrax ) and gilthead seabream Sparus aurata fillets cod Gadus morhua salmon Salmo salar during frozen storage in order to be able differentiate a fresh product from one that has been frozen. Analysis compounds performed these two types, after freezing/thawing...
Summary The initiation of starch granule formation is still poorly understood. However, the soluble synthase 4 ( SS 4) appears to be a major component this process since it required synthesize correct number granules in chloroplasts Arabidopsis thaliana plants. A yeast two‐hybrid screen allowed identification several putative interacting partners. We identified product At4g32190 locus as chloroplast‐targeted PROTEIN INVOLVED IN STARCH INITIATION (named PII 1). mutants devoid 1 display an...
Spore-forming bacteria of the Bacillus subtilis group are responsible for recurrent contamination processing lines in food industry which can lead to spoilage. The persistence B. would be due high resistance spores extreme environmental condition and their propensity contaminate surfaces. While it is well known that sporulation conditions modulate spore properties, little about effect on surface adhesion properties. Here, we studied impact 13 properties 168 spores. We showed Ca2+ or Mg2+...
BACKGROUND: The freshness of whiting was studied at five stages ice storage by comparing the analysis volatile compounds obtained through solid phase microextraction/gas chromatography/mass spectrometry (SPME/GC/MS) with two sensory methods. RESULTS: Of identified, 38 were analysed using a statistical multivariate approach and classified according to their role in estimation during as markers or spoilage. Regarding evolution presence absence individual compounds, three categories defined....
A new enzyme has been characterized in a cell‐free extract of Bifidobacterium bifidum that catalysed the reversible phosphorolytic cleavage β‐1,3‐galacto‐oligosaccharides. In presence P i , phosphorolysis reaction was favoured and accompanied by Walden reaction. Cleavage β‐glycosidic linkage gave an α‐galactoside derivative (α‐D‐galactose 1‐phosphate). The possesses high specificity for β‐D‐galactosido‐(1,3)‐ N ‐acetylglucosamine ‐acetylgalactosamine. This purified intracellular had...
Bacterial species from the Bacillus genus, including cereus and anthracis, synthesize secondary cell wall polymers (SCWP) covalently associated to peptidoglycan through a phospho-diester linkage. Although such components were observed in wide panel of B. anthracis strains, effect culture conditions or bacterial growth state on their synthesis has never been addressed. Herein we show that ATCC 14579 can not only one, as previously reported, but two structurally unrelated polysaccharides. The...
Bacillus species are a major economic and food safety concern of the industry because their spoilage-causing capability persistence. Their persistence is mainly due to ability form highly resistant spores adhering surfaces industrial equipment. Spores subtilis group surrounded by crust, superficial layer which plays key role in adhesion properties. However, knowledge composition structure this remains incomplete. Here, for first time, we identified nonulosonic acid (Leg) at bacterial ( B. )....
Producers of processed anchovies have developed hazard analysis and critical control points (HACCP) to guarantee the quality their products. Nonetheless there is a lack objective data determine products' shelf life. The product usually established on basis its safety organoleptic properties. These parameters were assessed by monitoring profiles volatile compounds quantitating six biogenic amines in samples two types during With regard amines, quantities below regulatory limits throughout...
Post-translational modifications (PTMs) constitute a huge group of chemical increasing the complexity proteomes living beings. PTMs have been discussed as potential anti-malarial drug targets due to their involvement in many cell processes. O-GlcNAcylation is widespread PTM found different organisms including Plasmodium falciparum. The aim this study was identify O-GlcNAcylated proteins P. falciparum, learn more about modification process and understand its eventual functions...
The spores of the Bacillus cereus group (B. cereus, anthracis, and thuringiensis) are surrounded by a paracrystalline flexible yet resistant layer called exosporium that plays major role in spore adhesion virulence. constituent its hairlike surface, trimerized glycoprotein BclA, is attached to basal through an N-terminal domain. It then followed repetitive collagen-like neck bearing globular head (C-terminal domain) promotes trimerization. region B. anthracis known be densely substituted...
Abstract BACKGROUND Fish is a highly perishable food, so it important to be able estimate its freshness ensure optimum quality for consumers. The present study describes the development of an SPME‐GC‐MS technique capable quantifying both trimethylamine ( TMA ) and dimethylamine DMA ), components what has been defined as partial volatile basic nitrogen PVB ‐N). This method was used, together with other reference methods, monitor storage cod fillets Gadus morhua conserved under melting ice....
ABSTRACT Although the ability of Bifidobacterium spp. to grow on fructose as a unique carbon source has been demonstrated, enzyme(s) needed incorporate into catabolic pathway hitherto not defined. This work demonstrates that intracellular is metabolized via fructose-6-P phosphoketolase and suggests fructokinase (Frk; EC 2.7.1.4) enzyme necessary sufficient for assimilation this route in longum . The B. A10C fructokinase-encoding gene ( frk ) was expressed Escherichia coli from pET28 vector...
Unfolded glycoproteins retained in the endoplasmic reticulum (ER) are degraded via ER-associated degradation (ERAD) pathway. These proteins subsequently transported to cytosol and by proteasomal complex. Although sequential events of ERAD well described, its regulation remains poorly understood. The cytosolic mannosidase, Man2C1, plays an essential role catabolism free oligomannosides, which released from proteins. We have investigated impact Man2C1 overexpression on protein glycosylation...
This study was designed to establish the presence and function of mucous layer surrounding spores Bacillus subtilis. First, an external variable thickness regularity often observed on B. subtilis spores. Further analyses were performed 98/7 surrounded by a thick layer. The mechanical removal did not affect their resistance heat or ability germinate but rendered spore less hydrophilic, more adherent stainless steel, resistant cleaning. mainly composed 6-deoxyhexoses, ie rhamnose,...
The presence of β-mannosides in their cell walls confers specific features on the pathogenic yeasts Candida albicans and glabrata compared with non-pathogenic yeasts. In present study, we investigated enzymatic properties Bmt1 (β-mannosyltransferase 1), a member recently identified β-mannosyltransferase family, from C. albicans. A recombinant soluble enzyme lacking N-terminal region was expressed as secreted protein methylotrophic yeast Pichia pastoris. parallel, functionalized natural...
niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français étrangers, laboratoires publics privés.
ABSTRACT Biofilm is a dynamic structure from which individual bacteria and micro-aggregates are released to subsequently colonize new niches by either detachment or dispersal. Screening of transposon mutant library identified genes associated with the alteration Klebsiella pneumoniae biofilm including fabR , encodes transcriptional regulator involved in membrane lipid homeostasis. An isogenic ∆ formed more than wild-type (WT) strain its trans-complemented strain. The thick round aggregates...
O-linked -N-acetylglucosaminylation or O-GlcNAcylation is a widespread post-translational modification that belongs to the large and heterogeneous group of glycosylations. The functions managed by are diverse include regulation transcription, replication, protein's fate, trafficking, signaling. More more evidences tend show deregulations in homeostasis involved etiology metabolic diseases, cancers neuropathologies. O-GlcNAc transferase OGT enzyme transfers N-acetylglucosamine residue onto...