A5070705823- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Polysaccharides and Plant Cell Walls
- Galectins and Cancer Biology
- Antifungal resistance and susceptibility
- Mycobacterium research and diagnosis
- Microbial Metabolites in Food Biotechnology
- Probiotics and Fermented Foods
- Radioactive contamination and transfer
- Helicobacter pylori-related gastroenterology studies
- Tuberculosis Research and Epidemiology
- Fungal Infections and Studies
- Radioactive element chemistry and processing
- Natural product bioactivities and synthesis
- Proteins in Food Systems
- Genomics and Phylogenetic Studies
- Antimicrobial Resistance in Staphylococcus
- Bacteriophages and microbial interactions
- Gut microbiota and health
- Proteoglycans and glycosaminoglycans research
- Radioactivity and Radon Measurements
- RNA and protein synthesis mechanisms
- Streptococcal Infections and Treatments
- Seaweed-derived Bioactive Compounds
- Legume Nitrogen Fixing Symbiosis
Inserm
2009-2025
Université de Lille
2014-2025
Centre Hospitalier Universitaire de Lille
2012-2025
Centre National de la Recherche Scientifique
2014-2025
Institut Pasteur de Lille
2021-2025
Unité de Glycobiologie Structurale et Fonctionnelle
2010-2020
Centre National pour la Recherche Scientifique et Technique (CNRST)
2005-2015
Université Lille Nord de France
2011-2014
Canadian Nautical Research Society
2014
Institut Pasteur
2009-2012
We have previously reported that 1-benzyl-2-acetamido-2-deoxy-α-d-galactopyranoside (GalNAcα-O-bn), an inhibitor of glycosylation, perturbed apical biosynthetic trafficking in polarized HT-29 cells suggesting involvement a lectin-based mechanism. Here, we identified galectin-4 as one the major components detergent-resistant membranes (DRMs) isolated from 5M12 cells. Galectin-4 was also found post-Golgi carrier vesicles. The functional role studied by using retrovirus-mediated RNA...
Mucin glycans were isolated from different regions of the normal human intestine (ileum, cecum, transverse and sigmoid colon, rectum) two individuals with ALeb blood group. A systematic study monosaccharides oligosaccharide alditols released by reductive β-elimination mucins was performed using gas chromatography, matrix-assisted laser desorption ionization time-of-flight mass spectrometry, nuclear magnetic resonance spectroscopy techniques. Important variations observed in mucin-associated...
The lipid composition of plasma membrane (PM) and the corresponding detergent-insoluble (DIM) fraction were analyzed with a specific focus on highly polar sphingolipids, so-called glycosyl inositol phosphorylceramides (GIPCs). Using tobacco (Nicotiana tabacum) 'Bright Yellow 2' cell suspension leaves, evidence is provided that GIPCs represent up to 40 mol % PM lipids. Comparative analysis DIMs showed an enrichment 2-hydroxylated very-long-chain fatty acid-containing polyglycosylated in DIMs....
Yeasts and their glycan components can have a beneficial or adverse effect on intestinal inflammation. Previous research has shown that the presence of Saccharomyces cerevisiae var. boulardii (Sb) reduces inflammation colonization by Candida albicans. The aim this study was to identify dietary yeasts, which comparable effects anti-C. albicans anti-inflammatory properties Sb assess capabilities yeast cell wall modulate Mice received single oral challenge C. were then given 1.5%...
Adhesion of Helicobacter pylori to the gastric mucosa is a necessary prerequisite for pathogenesis H. pylori–related diseases. In this study, we investigated GalNAcβ1-4GlcNAc motif (also known as N,N'-diacetyllactosediamine [lacdiNAc]) carried by MUC5AC mucins target bacterial binding human mucosa. The expression LacdiNAc was correlated with localization, and all strains tested adhered significantly motif. Proteomic analysis mutant construction allowed identification yet uncharacterized...
Helicobacter pylori infects more than half of the world's population. Although most patients are asymptomatic, persistent infection may cause chronic gastritis and gastric cancer. Adhesion bacteria to mucosa is a necessary prerequisite for pathogenesis H. pylori-related diseases mediated by mucin O-glycans. In order define which glycans be implicated in binding humans, we have characterized exact pattern glycosylation mucins. We identified that major component was always core 2-based glycan...
Enterococci are opportunistic pathogens responsible for hospital- and community-acquired infections. All enterococci produce a surface polysaccharide called EPA ( e nterococcal p olysaccharide ntigen) required biofilm formation, antibiotic resistance, pathogenesis. Despite the critical role of in cell growth division as major virulence factor, no information is available on its structure. Here, we report complete structure polymer produced by model strain E. faecalis V583. We describe...
Lipomannan (LM) and lipoarabinomannan (LAM) are major glycolipids present in the mycobacterial cell wall that able to modulate host immune response. In this study, we have undertaken structural determination of these important modulins inMycobacterium chelonae, a fast growing pathogenic species. One-dimensional two-dimensional NMR spectra were used demonstrate LM LAM from M. designated CheLM CheLAM, respectively, possess structures differ ones reported earlier other Analysis by gas...
The free-living nematode Caenorhabditis elegans is a relevant model for studies on the role of glycoconjugates during development multicellular organisms. Several genes coding glycosyltransferases involved in synthesis N- and O-linked glycans have already been isolated, but, apart from repetitive dimers glycosaminoglycans, no detailed structure either type component has published so far. This study aimed to establish structures major O-glycans synthesized by C. give an insight into...
Structural studies of cell wall components the pathogenic yeast Candida albicans have demonstrated presence beta-1,2-linked oligomannosides in phosphopeptidomannan and phospholipomannan. During C. infection, beta-1,2-oligomannosides play an important role host/pathogen interactions by acting as adhesins interfering with host immune response. Despite importance beta-1,2-oligomannosides, genes responsible for their synthesis not been identified. The main reason is that reference species...
Abstract Mouse CD1d-restricted NKT cells, including invariant (i)NKT are innate cells activated by glycolipid Ags and play important roles in the initiation regulation of immune responses. Through their ability to promptly produce large amounts Th1 and/or Th2 cytokines upon TCR engagement, iNKT exert crucial functions immune/inflammatory system during bacterial, protozoan, fungal, viral infections. However, metazoan parasite infection, which generally associated with strong responses, still...
Abstract The gastrointestinal tract is lined by a thick and complex layer of mucus that protects the mucosal epithelium from biochemical mechanical aggressions. This barrier confers protection against pathogens but also serves as binding site supports sheltered niche microbial adherence. carcinogenic bacteria Helicobacter pylori colonize stomach through to host glycans present in glycocalyx epithelial cells extracellular mucus. secreted MUC5AC mucin main component gastric layer,...
Although Mycobacterium kansasii has emerged as an important pathogen frequently encountered in immunocompromised patients, little is known about the mechanisms of M. pathogenicity. Lipoarabinomannan (LAM), a major mycobacterial cell wall lipoglycan, virulence factor for many mycobacteria, it modulates host immune response. Therefore, detailed structures LAM (KanLAM), well its biosynthetic precursor lipomannan (KanLM), were determined clinical strain isolated from human immunodeficiency...
The pathogenic yeast Candida albicans has the ability to synthesize unique sequences of beta-1,2-oligomannosides that act as adhesins, induce cytokine production, and generate protective antibodies. Depending on growth conditions, are associated with different carrier molecules in cell wall. Structural evidence been obtained for presence these residues polysaccharide moiety glycolipid, phospholipomannan (PLM). In this study, refinement purification techniques led large quantities PLM being...
Candida glabrata, like albicans, is an opportunistic yeast pathogen that has adapted to colonize all segments of the human gastrointestinal tract and vagina. The C. albicans cell wall expresses β-1,2-linked mannosides (β-Mans), promoting its adherence host cells tissues. Because β-Mans are also present in their role glabrata colonization virulence was investigated a murine model dextran sulfate sodium (DSS)-induced colitis. Five clustered genes encoding β-mannosyltransferases, BMT2-BMT6,...
The aim of this study was to characterize the Listeria monocytogenes biofilm and particularly nature carbohydrates in extracellular matrix culture supernatant versus cell wall carbohydrates. serotype 1/2a 4b strains were able form complex biofilms embedded an matrix. soluble from identified as teichoic acids, structurally identical acids. In addition, DSS 1130 BFA2 strain had a acid lacking N-acetyl glucosamine glycosylation due mutation lmo2550 gene. Consequently, we hypothesized that acids...
This paper describes structural characterization by NMR, MS and degradative studies of mucin glycans from normal human descending colon obtained freshly at autopsy. The saccharides were mainly based on core 3 (GlcNAcbeta1-3GalNAc). Among the terminal saccharide determinants Sd(a)/Cad-antigen-like structures prominent, Lewis x, sialyl x sulphated found as minor components, whereas blood group H A antigenic absent. markedly different those mucins cancers or cancer cell lines analysed so far,...
Summary Radiocaesium fixation in soils is reported to occur on frayed edge sites of micaceous minerals. The weathering mica acid may therefore influence the Cs + process and thereby mobility radiopollutant. We produced a laboratory model biotite → trioctahedral vermiculite oxidized hydroxy interlayered (HIV) quantified each mineral both fixed K –Ca 2+ background conditions. transformation was achieved through depletion by Na‐tetraphenylboron, oxidation with Br 2 Al‐intercalation using NaOH...
The free-living nematode Caenorhabditis elegans is a relevant model for studies on the role of glycoconjugates during development multicellular organisms. Several genes coding glycosyltransferases involved in synthesis N- and O-linked glycans have already been isolated, but, apart from repetitive dimers glycosaminoglycans, no detailed structure either type component has published so far. This study aimed to establish structures major O-glycans synthesized by C. give an insight into...
A method was developed for the determination of putative lectin activities cytokines. It involved immunoblotting measurement quantity these cytokines unbound to a series different immobilized glycoconjugates and displacement bound with oligosaccharides known structures. This allows demonstrating that following interleukins specifically recognize oligosaccharide structures in calcium-independent mechanism: interleukin-1α binds biantennary disialylatedN-glycan completed two Neu5Acα2–3...
Previous work has shown that treatment of HT-29 methotrexate (MTX) cells with benzyl-N-acetyl-α-d-galactosaminide results in profound changes mucin oligosaccharide chains. To analyse depth the effect this drug, we first determined structure chains synthesized by MTX and induced permanent drug exposure. Mucins from untreated contained nine monosialylated structures (core types 1, 2, 3 4) four disialylated (types 2 4). Core 1 predominated, particular NeuAcα2–3Galβ1–3GalNAc-ol. Exposure to days...