A5114803755- Mycorrhizal Fungi and Plant Interactions
- Legume Nitrogen Fixing Symbiosis
- Plant-Microbe Interactions and Immunity
- Plant Reproductive Biology
- Plant tissue culture and regeneration
- Photosynthetic Processes and Mechanisms
- Fungal Biology and Applications
- Transgenic Plants and Applications
- Plant Molecular Biology Research
- Plant Parasitism and Resistance
- Nematode management and characterization studies
- Heavy metals in environment
- Plant Stress Responses and Tolerance
- Lipid Membrane Structure and Behavior
- Cellular transport and secretion
- Plant Virus Research Studies
- Bioeconomy and Sustainability Development
- Lipid metabolism and biosynthesis
- Toxin Mechanisms and Immunotoxins
- Antimicrobial Peptides and Activities
- Nicotinic Acetylcholine Receptors Study
- Genomics and Phylogenetic Studies
- Forest Ecology and Biodiversity Studies
- Sphingolipid Metabolism and Signaling
- Healthcare and Venom Research
Agroécologie
2012-2023
Institut Agro Dijon
2014-2023
Université Bourgogne Franche-Comté
2018-2023
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2023
Centre National de la Recherche Scientifique
2009-2018
Université de Bourgogne
2012-2014
The lipid composition of plasma membrane (PM) and the corresponding detergent-insoluble (DIM) fraction were analyzed with a specific focus on highly polar sphingolipids, so-called glycosyl inositol phosphorylceramides (GIPCs). Using tobacco (Nicotiana tabacum) 'Bright Yellow 2' cell suspension leaves, evidence is provided that GIPCs represent up to 40 mol % PM lipids. Comparative analysis DIMs showed an enrichment 2-hydroxylated very-long-chain fatty acid-containing polyglycosylated in DIMs....
Abstract Background Arbuscular mycorrhizal (AM) fungi, which engage a mutualistic symbiosis with the roots of most plant species, have received much attention for their ability to alleviate heavy metal stress in plants, including cadmium (Cd). While molecular bases Cd tolerance displayed by plants been extensively analysed roots, very little is known regarding mechanisms legume aboveground organs can escape toxicity upon AM symbiosis. As model system address this question, we used Glomus...
The arbuscular mycorrhizal (AM) symbiosis belongs to the strategies plants have developed cope with adverse environmental conditions including contamination by heavy metals such as cadmium (Cd). In present work, we report on protective effect conferred AM model legume Medicago truncatula grown in presence of Cd, and 2-D-based proteomic approach further used compare proteomes M. roots either colonised or not fungus Glomus intraradices Cd-free Cd-contaminated substrates. results indicated that...
Plant NADPH oxidases, also known as respiratory burst oxidase homologues (RBOHs), have been identified a major source of reactive oxygen species (ROS) during plant–microbe interactions. The subcellular localization the tobacco (Nicotiana tabacum) ROS-producing enzyme RBOHD was examined in Bright Yellow-2 cells before and after elicitation with oomycete protein cryptogein using electron confocal microscopy. plasma membrane (PM) confirmed immuno-electron microscopy on purified PM vesicles...
The laterally heterogeneous plant plasma membrane (PM) is organized into finely controlled specialized areas that include membrane-ordered domains. Recently, the spatial distribution of such domains within PM has been identified as playing a key role in cell responses to environmental challenges. To examine order at local level, BY-2 tobacco suspension PMs were labelled with an environment-sensitive probe (di-4-ANEPPDHQ). Four experimental models compared identify mechanisms and components...
Endocytosis has been recently implicated in the signaling network associated with recognition of microbes by plants. In a previous study, we showed that elicitor cryptogein was able to induce clathrin-mediated endocytosis (CME) tobacco suspension cells. Herein, investigate further induced CME means GFP-tagged clathrin light chain and inhibitor, hub domain heavy chain. Hub constitutive expression does affect neither cell growth nor but abolishes cryptogein-induced CME. Such an inhibition no...
The proteinaceous elicitor cryptogein triggers defence reactions in Nicotiana tabacum (tobacco) through a signalling cascade, including the early production of reactive oxygen species (ROS) by plasma membrane (PM)-located tobacco respiratory burst oxidase homologue D (NtRbohD). Sphingolipid long-chain bases (LCBs) are emerging as potent positive regulators plant defence-related mechanisms. This led us to question whether both LCBs and their phosphorylated derivatives (LCB-Ps) involved...
Expression profiling of two paralogous arbuscular mycorrhizal (AM)-specific blue copper-binding gene (MtBcp1a and MtBcp1b) isoforms was performed by real-time quantitative polymerase chain reaction in wild-type Medicago truncatula Jemalong 5 (J5) during the development with Glomus intraradices for up to 7 weeks. Time-course analysis J5 showed that expression both MtBcp1 genes increased continuously correlated strongly colonization intensity arbuscule content. MtPT4, selected as a reference...
Abstract The plant plasma membrane (PM) plays a key role in nutrition, cell homeostasis, perception of environmental signals, and set-up appropriate adaptive responses. An exhaustive quantitative description the whole set lipids proteins constituting PM is thus necessary to understand how way these components, are organized interact together, allow fulfill such essential physiological functions. Here we provide by state-of-the-art approaches first combined reference lipidome proteome from...