A5005055940- Legume Nitrogen Fixing Symbiosis
- Plant-Microbe Interactions and Immunity
- Plant Virus Research Studies
- Photosynthetic Processes and Mechanisms
- Plant Reproductive Biology
- Polysaccharides and Plant Cell Walls
- Plant Molecular Biology Research
- Toxin Mechanisms and Immunotoxins
- Lipid Membrane Structure and Behavior
- Wheat and Barley Genetics and Pathology
- Lipid metabolism and biosynthesis
- Plant Stress Responses and Tolerance
- Bacteriophages and microbial interactions
- Plant Pathogenic Bacteria Studies
- Plant Disease Resistance and Genetics
- Botanical Research and Chemistry
- Plant nutrient uptake and metabolism
- Transgenic Plants and Applications
- Sphingolipid Metabolism and Signaling
- Antimicrobial Peptides and Activities
- Advanced Fluorescence Microscopy Techniques
- Algal biology and biofuel production
- Plant tissue culture and regeneration
- Advanced Electron Microscopy Techniques and Applications
- Cell Image Analysis Techniques
Université de Bordeaux
2014-2024
Laboratoire de Biogenèse Membranaire
2014-2024
Centre National de la Recherche Scientifique
2014-2024
Institute of Plant Molecular Biology
2023-2024
University of Tübingen
2021-2024
University of Zurich
2018-2023
Sainsbury Laboratory
2020-2022
University of East Anglia
2020-2022
Norwich Research Park
2020-2022
Austrian Academy of Sciences
2022
Spatial partitioning is a propensity of biological systems orchestrating cell activities in space and time. The dynamic regulation plasma membrane nano-environments has recently emerged as key fundamental aspect plant signaling, but the molecular components governing it are still mostly unclear. receptor kinase FERONIA (FER) controls ligand-induced complex formation immune FLAGELLIN SENSING 2 (FLS2) with its co-receptor BRASSINOSTEROID-INSENSITIVE 1-ASSOCIATED KINASE 1 (BAK1), perception...
The lipid composition of plasma membrane (PM) and the corresponding detergent-insoluble (DIM) fraction were analyzed with a specific focus on highly polar sphingolipids, so-called glycosyl inositol phosphorylceramides (GIPCs). Using tobacco (Nicotiana tabacum) 'Bright Yellow 2' cell suspension leaves, evidence is provided that GIPCs represent up to 40 mol % PM lipids. Comparative analysis DIMs showed an enrichment 2-hydroxylated very-long-chain fatty acid-containing polyglycosylated in DIMs....
Plasma Membrane is the primary structure for adjusting to ever changing conditions. PM sub-compartmentalization in domains thought orchestrate signaling. Yet, mechanisms governing membrane organization are mostly uncharacterized. The plant-specific REMORINs proteins regulating hormonal crosstalk and host invasion. REMs best-characterized nanodomain markers via an uncharacterized moiety called REMORIN C-terminal Anchor. By coupling biophysical methods, super-resolution microscopy physiology,...
Plant aerial organs are coated with cuticular waxes, a hydrophobic layer that primarily serves as waterproofing barrier. Cuticular wax is mixture of aliphatic very-long-chain molecules, ranging from 22 to 48 carbons, produced in the endoplasmic reticulum epidermal cells. Among all components, alkanes represent up 80% total Arabidopsis (Arabidopsis thaliana) leaves. Odd-numbered and their derivatives through alkane-forming pathway. Although chemical reactions this pathway have been well...
Abstract Plasma membrane-associated and intracellular proteins protein complexes play a pivotal role in pathogen recognition disease resistance signaling plants animals. The two predominant families perceiving plant pathogens are receptor-like kinases nucleotide binding-leucine-rich repeat receptors (NLR), which often confer race-specific resistance. Leaf rust is one of the most prevalent devastating wheat diseases. Here, we clone leaf gene Lr14a from hexaploid wheat. cloning aided by...
Plants respond to pathogens through dynamic regulation of plasma membrane-bound signaling pathways. To date, how the plant membrane is involved in responses viruses mostly unknown. Here, we show that cells sense Potato virus X (PVX) COAT PROTEIN and TRIPLE GENE BLOCK 1 proteins subsequently trigger activation a calcium-dependent kinase. We Arabidopsis thaliana CALCIUM-DEPENDENT KINASE 3-interacts with group REMORINs vivo, phosphorylates intrinsically disordered N-terminal domain Group...
Cells maintain a constant dialog between the extracellular matrix and their plasma membrane to fine tune signal transduction processes. We found that receptor kinase FERONIA (FER), which is proposed cell wall sensor, modulates phosphatidylserine accumulation nano-organization, key regulator of Rho GTPase signaling in Arabidopsis. demonstrate FER required for both Rho-of-Plant 6 (ROP6) nano-partitioning at downstream production reactive oxygen species upon hyperosmotic stimulus. Genetic...
Plant receptor kinases are key transducers of extracellular stimuli, such as the presence beneficial or pathogenic microbes secreted signaling molecules. Receptor regulated by numerous post-translational modifications.
The Triple Gene Block 1 (TGBp1) protein encoded by the Potato virus X is a multifunctional that acts as suppressor of RNA silencing or facilitates passage from cell to promoting plasmodesmata opening. We previously showed membrane raft StRemorin1.3 able impair PVX infection. Here, we show overexpressed does not activity TGBp1, but affects its ability increase permeability. A similar effect on permeability was observed with other movement proteins, suggesting REM general regulator...
Super-resolution microscopy (SRM) approaches revolutionize cell biology by providing insights into the nanoscale organization and dynamics of macromolecular assemblies single molecules in living cells. A major hurdle limiting SRM democratization is post-acquisition data analysis which often complex time-consuming. Here, we present OneFlowTraX, a user-friendly open-source software dedicated to single-molecule localization (SMLM) such as single-particle tracking photoactivated (sptPALM)....
Abstract Single-molecule imaging promises the observation of individual molecules at work in living cells 1,2 . In plants, however, tracking single is generally limited to mere hundred milliseconds 3–5 , making it virtually impossible observe live dynamic cellular events with molecular resolution. Here, we introduce photochromic reversion which uses EOS fluorescent protein’s dark state upon blue light illumination 6 thereby stabilizing and extending single-molecule particle photoactivated...
Plant viruses represent a risk to agricultural production and as only few treatments exist, it is urgent identify resistance mechanisms factors. In plant immunity, plasma membrane (PM)-localized proteins play an essential role in sensing the extracellular threat presented by bacteria, fungi or herbivores. Viruses are intracellular pathogens such of PM detection against often overlooked. We investigated partially PM-bound Calcium-dependent protein kinase 3 (CPK3) viral infection we discovered...
Abstract Plant growth and development are modulated by both biotic abiotic stress. Increasing evidence suggests that cellular integration of the corresponding signals occurs within preformed hubs at plasma membrane called nanodomains. These sub-compartments organized multivalent molecular scaffold proteins, such as remorins. Here, we demonstrate Group 1 remorins form a hetero-oligomeric complex membrane. While these functionally redundant for some pathways their multivalency also allows...
Plant cells survey and modulate their cell wall to control shape anisotropic growth. Signaling mediated by the plant steroid hormones brassinosteroids (BR) plays a central role in coordinating status growth, alterations BR feedback loop leads life-threatening defects tissue cellular integrity. How of is relayed signaling remains largely unclear. Increasing evidence shows that RAPID ALKALANIZATION FACTORs (RALFs), class secreted peptides, play structural roles at surface. Here we show...
Eukaryotic Δ6-desaturases are microsomal enzymes that balance the synthesis of ω-3 and ω-6 C18-polyunsaturated fatty acids (C18-PUFAs) according to their specificity. In several microalgae, including Ostreococcus tauri, plastidic C18-PUFAs strictly regulated by environmental cues suggesting an autonomous control Δ6-desaturation PUFAs. Here, we identified two putative front-end Δ6/Δ8-desaturases from O. tauri that, together with homologs, cluster apart other characterized Δ6-desaturases. Both...