A5005096116- Plant Reproductive Biology
- Plant Molecular Biology Research
- Polysaccharides and Plant Cell Walls
- Plant nutrient uptake and metabolism
- Toxin Mechanisms and Immunotoxins
- Cellular transport and secretion
- Plant tissue culture and regeneration
- Photosynthetic Processes and Mechanisms
- Plant-Microbe Interactions and Immunity
- Food Quality and Safety Studies
- Soybean genetics and cultivation
- Polymer-Based Agricultural Enhancements
- AI in cancer detection
- Plant responses to water stress
- Lipid Membrane Structure and Behavior
- Seedling growth and survival studies
- Cancer-related gene regulation
- Biochemical and Structural Characterization
- Autophagy in Disease and Therapy
- Supramolecular Self-Assembly in Materials
- Plant Genetic and Mutation Studies
- Flowering Plant Growth and Cultivation
- Islanding Detection in Power Systems
- Phytase and its Applications
- Legume Nitrogen Fixing Symbiosis
BOKU University
2015-2024
University of Freiburg
2021-2024
Significance Cellular growth in plants is constrained by cell walls; hence, loosening these structures required for growth. The long-standing acid theory links auxin signaling, apoplastic pH homeostasis, and cellular expansion, providing a conceptual framework expansion plant shoots. Intriguingly, this model remains heavily debated roots. Here, we present fluorescent dye that allows the correlation of size at resolution Arabidopsis thaliana . This enabled us to elucidate complex involvement...
Article8 March 2019Open Access Source DataTransparent process Extracellular matrix sensing by FERONIA and Leucine-Rich Repeat Extensins controls vacuolar expansion during cellular elongation in Arabidopsis thaliana Kai Dünser orcid.org/0000-0002-8974-2072 Department of Applied Genetics Cell Biology, University Natural Resources Life Sciences (BOKU), Vienna, Austria Search for more papers this author Shibu Gupta orcid.org/0000-0003-0412-7423 Plant Microbial Zurich, Switzerland Aline Herger...
Spatial partitioning is a propensity of biological systems orchestrating cell activities in space and time. The dynamic regulation plasma membrane nano-environments has recently emerged as key fundamental aspect plant signaling, but the molecular components governing it are still mostly unclear. receptor kinase FERONIA (FER) controls ligand-induced complex formation immune FLAGELLIN SENSING 2 (FLS2) with its co-receptor BRASSINOSTEROID-INSENSITIVE 1-ASSOCIATED KINASE 1 (BAK1), perception...
The control of cellular growth is central to multicellular patterning. In plants, the encapsulating cell wall literally binds neighbouring cells each other and limits sliding/migration. contrast its developmental importance, regulation poorly understood in plants. Here, we reveal that phytohormone auxin impacts on shape biggest plant organelle, vacuole. TIR1/AFBs-dependent signalling posttranslationally controls protein abundance vacuolar SNARE components. Genetic pharmacological...
Abstract Directional organ growth allows the plant root system to strategically cover its surroundings. Intercellular auxin transport is aligned with gravity vector in primary tips, facilitating downward bending at lower flank. Here we show that cytokinin signaling functions as a lateral specific anti-gravitropic component, promoting radial distribution of system. We performed genome-wide association study and reveal signal peptide processing Cytokinin Oxidase 2 (CKX2) affects enzymatic...
The extracellular matrix plays an integrative role in cellular responses plants, but its contribution to the signalling of ligands largely remains be explored. Rapid alkalinisation factors (RALFs) are peptide hormones that play pivotal roles various physiological processes. Here, we address a crucial connection between de-methylesterification machinery cell wall component pectin and RALF1 activity. Pectin is polysaccharide, contributing structural integrity wall. Our data illustrate...
The regulation of cellular growth is vital importance for embryonic and postembryonic patterning. Growth in the epidermis has organ rates roots shoots, proposing epidermal cells as an interesting model regulation. Here we assessed whether root a suitable system to address cell size determination. In Arabidopsis thaliana L., are regularly spaced neighbouring tricho- (root hair) atrichoblast (non-hair) cells, showing already distinct meristem. We determined sizes meristem at onset elongation,...
The vacuole has a space-filling function, allowing particularly rapid plant cell expansion with very little increase in cytosolic content (Löfke et al., 2015; Scheuring 2016; Dünser 2019). Despite its importance for size determination plants, is known about the mechanisms that define vacuolar size. Here, we show cellular and expansions are coordinated. By developing pharmacological tool, enabled investigation of membrane delivery to during expansion. Our data reveal endocytic sorting from...
The phytohormone auxin is a vital growth regulator in plants. In the root epidermis steers organ growth. However, mechanisms that allow adjacent tissues to integrate are largely unknown. Here, focus on neighbouring epidermal assess integration of auxin-related responses. pharmacologic, genetic, and live-cell imaging approaches reveal PIN2 efflux carriers differentially controlled tricho- atrichoblast cells. proteins show lower abundance at plasma membrane trichoblast cells, despite showing...
Many signal perception mechanisms are connected to Ca2+-based second messenger signaling modulate specific cellular responses. The well-characterized plant hormone auxin elicits a very rapid Ca2+ signal. However, the targets of auxin-induced largely unknown. Here, we screened biologically annotated chemical library for inhibitors entry in cell suspensions better understand molecular mechanism and explore physiological relevance transduction. Using this approach, defined set diverse, small...
Size control is a fundamental question in biology, showing incremental complexity plants, whose cells possess rigid cell wall. The phytohormone auxin vital growth regulator with central importance for differential control. Our results indicate that auxin-reliant programs affect the molecular of xyloglucans, major type wall hemicellulose eudicots. Auxin-dependent induction and repression coincide reduced enhanced respectively. In agreement proposed function control, genetic interference...
The extracellular matrix plays an integrative role in cellular responses plants, but its contribution to the signalling of ligands largely remains be explored. RAPID ALKALINIZATION FACTORs (RALFs) are peptide hormones that play pivotal roles various physiological processes. Here, we address a crucial connection between demethylation machinery cell wall component pectin and RALF1 activity. Pectin is polysaccharide, contributing structural integrity wall. Our data illustrate pharmacological...
The extracellular matrix plays an integrative role in cellular responses plants, but its contribution to the signalling of ligands largely remains be explored. Rapid alkalinisation factors (RALFs) are peptide hormones that play pivotal roles various physiological processes. Here, we address a crucial connection between de-methylesterification machinery cell wall component pectin and RALF1 activity. Pectin is polysaccharide, contributing structural integrity wall. Our data illustrate...
Cellular elongation requires the defined coordination of intra- and extracellular processes. The vacuole is biggest plant organelle its dimension has a role in limiting cell expansion (Löfke et al., 2015; Scheuring 2016). We reveal that increase vacuolar occupancy enables cellular with relatively little enlargement cytosole. It remains, however, completely unknown how size coordinated other growth-relevant Intriguingly, we show constraints impact on intracellular vacuole. underlying wall...
Abstract The extracellular matrix plays an integrative role in cellular responses plants, but its contribution to the signalling of ligands largely remains be explored. RAPID ALKALINIZATION FACTORs (RALFs) are peptide hormones that play pivotal roles various physiological processes. Here, we address a crucial connection between de-methylesterification machinery cell wall component pectin and RALF1 activity. Pectin is polysaccharide, contributing structural integrity wall. Our data illustrate...
ABSTRACT Spatial partitioning is a propensity of biological systems orchestrating cell activities in space and time. The dynamic regulation plasma membrane nano-environments has recently emerged as key fundamental aspect plant signaling, but the molecular components governing it are still mostly unclear. receptor kinase FERONIA (FER) controls complex formation immune FLAGELLIN SENSING 2 (FLS2) with its co-receptor BRASSINOSTEROID INSENSITIVE 1-ASSOCIATED KINASE 1 (BAK1), this function...
Size control is a fundamental question in biology, showing incremental complexity case of plants whose cells possess rigid cell wall. The phytohormone auxin vital growth regulator with central importance for differential control. Here we show that inducing and repressing conditions correlate reduced enhanced extracellular xyloglucans, respectively. In agreement, genetic interference xyloglucan distinctly modulates auxin-dependent rates. Our work proposes an auxin-dependent, spatially defined...