A5012801870- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Cellular transport and secretion
- Light effects on plants
- Polysaccharides and Plant Cell Walls
- Plant Stress Responses and Tolerance
- Plant Gene Expression Analysis
- Plant Growth Enhancement Techniques
- Plant Physiology and Cultivation Studies
BOKU University
2013-2024
Romanian University of Sciences and Arts "Gheorghe Cristea"
2023-2024
Ghent University
2010-2014
Vlaams Instituut voor Biotechnologie
2011-2014
VIB-UGent Center for Plant Systems Biology
2010-2013
University of Tübingen
2010
Article8 March 2019Open Access Source DataTransparent process Extracellular matrix sensing by FERONIA and Leucine-Rich Repeat Extensins controls vacuolar expansion during cellular elongation in Arabidopsis thaliana Kai Dünser orcid.org/0000-0002-8974-2072 Department of Applied Genetics Cell Biology, University Natural Resources Life Sciences (BOKU), Vienna, Austria Search for more papers this author Shibu Gupta orcid.org/0000-0003-0412-7423 Plant Microbial Zurich, Switzerland Aline Herger...
Abstract Plant vacuoles are essential multifunctional organelles largely distinct from similar in other eukaryotes. Embryo protein storage and the lytic that perform a general degradation function best characterized, but little is known about biogenesis transition between these vacuolar types. Here, we designed fluorescent marker–based forward genetic screen Arabidopsis thaliana identified affected trafficking2 (pat2) mutant, whose display altered morphology accumulation of proteins. Unlike...
Temperature modulates growth and development throughout the entire lifecycle of a plant. High temperature (HT) triggers auxin biosynthesis-dependent in aerial tissues. On other hand, contribution to HT-induced root is currently under debate. Here we show that putative intracellular carrier PIN-LIKES 6 (PILS6) negative regulator organ its abundance highly sensitive HT. PILS6 localizes endoplasmic reticulum limits nuclear availability auxin, consequently reducing signaling output. HT represses...
Constitutive endocytic recycling is a crucial mechanism allowing regulation of the activity proteins at plasma membrane and for rapid changes in their localization, as demonstrated plants PIN-FORMED (PIN) proteins, auxin transporters. To identify novel molecular components recycling, mainly exocytosis, we designed PIN1-green fluorescent protein fluorescence imaging–based forward genetic screen Arabidopsis thaliana mutants that showed increased intracellular accumulation cargos response to...
PIN-FORMED (PIN) proteins localize asymmetrically at the plasma membrane and mediate intercellular polar transport of plant hormone auxin that is crucial for a multitude developmental processes in plants. PIN localization under extensive control by environmental or cues, but mechanisms regulating are not fully understood. Here we show early endosomal components ARF GEF BEN1 newly identified Sec1/Munc18 family protein BEN2 involved distinct steps trafficking. collectively required...
Auxin and brassinosteroids (BR) are crucial growth regulators display overlapping functions during plant development. Here, we reveal an alternative phytohormone crosstalk mechanism, revealing that BR signaling controls PIN-LIKES (PILS)-dependent nuclear abundance of auxin. We performed a forward genetic screen for imperial pils (imp) mutants enhance the overexpression phenotypes PILS5 putative intracellular auxin transport facilitator. report imp1 mutant is defective in BR-receptor...
Significance Polyphosphoinositides (PPIs) are derivatives of the membrane lipid phosphatidylinositol that occur in minor amounts eukaryotic membranes. PPIs have regulatory effects on various cellular processes, but their roles plants currently not well-understood. Plant growth relies largely turgor-driven cell expansion, which at subcellular level is linked to vacuolar dynamics. We identified an unknown subgroup tonoplast-associated enzymes from Arabidopsis thaliana , suppressor actin 2...
Correct positioning of membrane proteins is an essential process in eukaryotic organisms. The plant hormone auxin distributed through intercellular transport and triggers various cellular responses. Auxin transporters the PIN-FORMED (PIN) family localize asymmetrically at plasma (PM) mediate directional between cells. A fungal toxin, brefeldin (BFA), inhibits a subset guanine nucleotide exchange factors for ADP-ribosylation factor small GTPases (ARF GEFs) including GNOM, which plays major...
ABSTRACT Multiple internal and external signals modulate the metabolism, intercellular transport signaling of phytohormone auxin. Considering this complexity, it remains largely unknown how plant cells monitor ensure homeostasis auxin responses. PIN-LIKES (PILS) intracellular facilitators at endoplasmic reticulum are suitable candidates to buffer cellular responses because they limit nuclear abundance We used forward genetics identify gloomy shiny pils (gasp) mutants that define PILS6...
Extreme environmental conditions eventually limit plant growth [J. R. Dinneny, Annu. Rev. Cell Dev. Biol. 35 , 1–19 (2019), N. Gigli-Bisceglia, C. Testerink, Curr. Opin. Plant 64 102120 (2021)]. Here, we reveal a mechanism that enables multiple external cues to get integrated into auxin-dependent programs in Arabidopsis thaliana . Our forward genetics approach on dark-grown hypocotyls uncovered an imbalance membrane lipids enhances the protein abundance of PIN-LIKES (PILS) [E. Barbez et al....
The phytohormone abscisic acid (ABA) functions in the control of plant stress responses, particularly drought stress. A significant mechanism attenuating and terminating ABA signals involves regulated protein turnover, with certain receptors, despite their main presence cytosol nucleus, subjected to vacuolar degradation via Endosomal Sorting Complex Required for Transport (ESCRT) machinery. Collectively our findings show that discrete TOM1-LIKE (TOL) proteins, which are functional ESCRT-0...
Plants adjust their architecture to a constantly changing environment, requiring adaptation of differential growth. Despite importance, molecular switches, which define growth transitions, are largely unknown. Apical hook development in dark grown Arabidopsis thaliana (A. thaliana) seedlings serves as suitable model for transition plants. Here, we show that the phytohormone auxin counteracts light-induced during apical opening. We, subsequently, identified genes inversely regulated by light...
Global warming is threatening plant productivity, because growth highly sensitive to elevated temperatures. High temperature (HT) triggers the auxin biosynthesis-dependent in aerial tissues. On other hand, contribution of HT-induced root currently under debate. Here we show that putative intracellular carrier PIN-LIKES 6 (PILS6) a negative regulator organ and its abundance HT. PILS6 localises endoplasmic reticulum (ER) limits nuclear availability auxin, consequently reducing signalling...
Abstract Auxin and brassinosteroids (BR) are crucial growth regulators display overlapping functions during plant development. Here, we reveal an alternative phytohormone crosstalk mechanism, revealing that brassinosteroid signaling controls nuclear abundance of auxin. We performed a forward genetic screen for imperial pils ( imp ) mutants enhance the overexpression phenotypes PIN-LIKES (PILS) putative intracellular auxin transport facilitator. Here report imp1 mutant is defective in...
Abstract Extreme environmental conditions eventually limit plant growth (1, 2). Here we reveal an unprecedented mechanism that enables multiple external cues to get integrated into auxin-dependent programs in Arabidopsis thaliana . Our forward genetics approach on dark grown hypocotyls uncovered imbalance membrane lipids enhances the protein abundance of PIN-LIKES (PILS) (3–5) auxin transport facilitators at endoplasmic reticulum (ER), which thereby limits nuclear signaling and rates. We...
Abstract Auxin is a crucial regulator of plant growth and development. Multiple internal external signals converge at the regulation auxin metabolism, intercellular transport, signaling (Pernisova Vernoux, 2021; Anfang Shani, 2021). Considering this complexity, it remains largely unknown how cells monitor ensure homeostasis responses. PIN-LIKES (PILS) intracellular transport facilitators endoplasmic reticulum (ER) are suitable candidates to buffer cellular responses, because they limit...