A5071681621- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Polish Historical and Cultural Studies
- Historical and Cultural Studies of Poland
- Plant Parasitism and Resistance
- Legume Nitrogen Fixing Symbiosis
- Central European Literary Studies
- Medieval European History and Architecture
- Plant and animal studies
- Urban Development and Cultural Heritage
- Plant-Microbe Interactions and Immunity
- Plant Gene Expression Analysis
- RNA Research and Splicing
- Geology and Environmental Impact Studies
- Integrated Water Resources Management
- Plant tissue culture and regeneration
- Language and Culture
- Aquatic Ecosystems and Phytoplankton Dynamics
- European Politics and Security
- Biocrusts and Microbial Ecology
- MicroRNA in disease regulation
- Education and Cultural Studies
- Social Issues in Poland
Central European Institute of Technology – Masaryk University
2015-2024
Masaryk University
2015-2024
Central European Institute of Technology
2014-2024
University of Zielona Góra
2013-2017
VIB-UGent Center for Plant Systems Biology
2011-2015
Ghent University
2009-2015
Vlaams Instituut voor Biotechnologie
2009
The plant hormone auxin plays a crucial role in regulating development and architecture. directional distribution within tissues depends on PIN transporters that are polarly localized the plasma membrane. polarity resulting flow directionality mediated by antagonistic actions of PINOID kinase protein phosphatase 2A. However, contribution phosphorylation to polar sorting is still unclear. Here, we identified an evolutionarily conserved site central hydrophilic loop proteins important for...
Cell polarity is a fundamental feature of all multicellular organisms. PIN auxin transporters are important cell markers that play crucial roles in plethora developmental processes plants. Here, to identify components involved establishment and maintenance plants, we performed forward genetic screening PIN2:PIN1-HA;pin2 Arabidopsis (Arabidopsis thaliana) which ectopically express predominantly basally localized PIN1 root epidermal cells, leading agravitropic growth. We identified the...
The plant hormone auxin is a key regulator of growth and development. Auxin levels are sensed interpreted by distinct receptor systems that activate broad range cellular responses. Auxin-Binding Protein1 (ABP1) has been identified based on its ability to bind with high affinity prime candidate for the extracellular responsible mediating effects, in particular, fast non-transcriptional ones. Contradictory genetic studies suggested prominent or no importance ABP1 many developmental processes....
Correct positioning of membrane proteins is an essential process in eukaryotic organisms. The plant hormone auxin distributed through intercellular transport and triggers various cellular responses. Auxin transporters the PIN-FORMED (PIN) family localize asymmetrically at plasma (PM) mediate directional between cells. A fungal toxin, brefeldin (BFA), inhibits a subset guanine nucleotide exchange factors for ADP-ribosylation factor small GTPases (ARF GEFs) including GNOM, which plays major...
Auxin is an important regulator of plant ontogenies including embryo development and the exogenous application this phytohormone has been found to be necessary for induction embryogenic response in explants that have cultured vitro. However, present study, we show treatment Arabidopsis with trichostatin A (TSA), which a chemical inhibitor histone deacetylases, induces somatic embryogenesis (SE) without auxin. We TSA-treated generated embryos developed efficiently on adaxial side cotyledons,...
Abstract The phytohormone auxin is a major determinant and regulatory component important for plant development. Auxin transport between cells mediated by complex system of transporters such as AUX1/LAX, PIN, ABCB proteins, their localization activity thought to be influenced phosphatases kinases. Flavonols have been shown alter changes in flavonol accumulation the Arabidopsis thaliana rol1-2 mutant cause defects seedling A new mutation ROOTS CURL IN NPA 1 (RCN1 ), encoding subunit...
Strigolactones (SLs) are a relatively recent addition to the list of plant hormones that control different aspects development. SL signalling is perceived by an α/β hydrolase, DWARF 14 (D14). A close homolog D14, KARRIKIN INSENSTIVE2 (KAI2), involved in perception uncharacterized molecule called karrikin (KAR). Recent studies Arabidopsis identified SUPPRESSOR OF MAX2 1 (SMAX1) and SMAX1-LIKE 7 (SMXL7) be potential SCF–MAX2 complex-mediated proteasome targets KAI2 respectively. Genetic on...
Redirection of intercellular auxin fluxes via relocalization the PIN-FORMED 3 (PIN3) and PIN7 efflux carriers has been suggested to be necessary for root gravitropic response. Cytokinins have also proposed play a role in controlling gravitropism, but conclusive evidence is lacking. We present detailed study dynamics bending early after gravistimulation, which revealed delayed response transgenic lines with depleted endogenous cytokinins (Pro35S:AtCKX) cytokinin signaling mutants....
Leaf venation develops complex patterns in angiosperms, but the mechanism underlying this process is largely unknown. To elucidate molecular mechanisms governing vein pattern formation, we previously isolated vascular network defective (van) mutants that displayed discontinuities. Here, report phenotypic analysis of van4 mutants, and identify characterize VAN4 gene. Detailed shows are procambium cell differentiation subsequent differentiation. Reduced shoot root growth observed suggesting...
Abstract PIN proteins establish the auxin concentration gradient, which coordinates plant growth. PIN1-4 and 7 localized at plasma membrane (PM) facilitate polar transport while endoplasmic reticulum (ER) PIN5 PIN8 maintain intracellular homeostasis. Although an antagonistic activity of in regulating homeostasis other developmental events have been reported, topology these proteins, might be a basis for their function, is poorly understood. In this study we optimized digitonin based...
The trafficking of subcellular cargos in eukaryotic cells crucially depends on vesicle budding, a process mediated by ARF-GEFs (ADP-ribosylation factor guanine nucleotide exchange factors). In plants, play essential roles endocytosis, vacuolar trafficking, recycling, secretion, and polar trafficking. Moreover, they are important for plant development, mainly through controlling the localization PIN-FORMED transporters hormone auxin. Here, using chemical genetics screen Arabidopsis thaliana,...
To compensate for their sessile lifestyle, plants developed several responses to exogenous changes. One of the previously investigated and not yet fully understood adaptations occurs at level early subcellular trafficking, which needs be rapidly adjusted maintain cellular homeostasis membrane integrity under osmotic stress conditions. form a vesicle, deformed, is ensured by multiple factors, including activity specific proteins, such as flippases from family P4-ATPases. The pumps actively...
Small RNAs (smRNA, 19–25 nucleotides long), which are transcribed by RNA polymerase II, regulate the expression of genes involved in a multitude processes eukaryotes. miRNA biogenesis and proteins pathway differ across plant animal lineages. The major constituting pathway, namely, Dicers (DCL/DCR) Argonautes (AGOs), have been extensively studied. However, accessory (DAWDLE (DDL), SERRATE (SE), TOUGH (TGH)) that differs two lineages remain largely uncharacterized. We present first detailed...
Microcystin-LR (MCY-LR) is a heptapeptide toxin produced mainly by freshwater cyanobacteria. It strongly inhibits protein phosphatases PP2A and PP1. Functioning of the PIN family auxin efflux carriers crucial for plant ontogenesis their functions depend on reversible phosphorylation. We aimed to reveal adverse effects MCY-LR distribution in Arabidopsis roots its consequences root development. Relatively short-term (24 h) treatments decreased levels PIN1, PIN2 PIN7, but not PIN3 tips primary...
Much of plant development depends on cell-to-cell redistribution the hormone auxin, which is facilitated by plasma membrane (PM) localized PIN FORMED (PIN) proteins. Auxin export activity, developmental roles, subcellular trafficking, and polarity PINs have been well studied, but their structure remains elusive besides a rough outline that they contain two groups 5 alpha-helices connected large hydrophilic loop (HL). Here, we focus PIN1 HL as could produce it in sufficient quantities for...
The NGATHA (NGA) transcription factor (TF) belongs to the ABI3/VP1 (RAV) transcriptional subfamily, a subgroup of B3 superfamily, which is relatively well-studied in Arabidopsis. However, limited data are available on contributions NGA TF other plant species. In this study, 207 gene family members were identified from genome-wide search against Arabidopsis thaliana genome 18 dicots and seven monocots. phylogenetic sequence alignment analyses divided genes into different clusters revealed...