A5058909759- Plant Stress Responses and Tolerance
- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- Plant Gene Expression Analysis
- Insect-Plant Interactions and Control
- Plant nutrient uptake and metabolism
- Plant responses to water stress
- Plant Parasitism and Resistance
- Plant-Microbe Interactions and Immunity
- Plant responses to elevated CO2
- Redox biology and oxidative stress
- Allelopathy and phytotoxic interactions
- Plant and animal studies
- Plant biochemistry and biosynthesis
- Phytochemicals and Antioxidant Activities
- Crystallization and Solubility Studies
- Potato Plant Research
- Nitrogen and Sulfur Effects on Brassica
- Genomics, phytochemicals, and oxidative stress
- X-ray Diffraction in Crystallography
- Catalytic Alkyne Reactions
- Cyclopropane Reaction Mechanisms
- S100 Proteins and Annexins
- Plant Genetic and Mutation Studies
- Pesticide and Herbicide Environmental Studies
Mendel University in Brno
2019-2025
VIB-UGent Center for Plant Systems Biology
2013-2024
Ghent University
2013-2024
Ghent University Hospital
2018
Vlaams Instituut voor Biotechnologie
2016
James Hutton Institute
2011-2013
University of Leeds
2010-2013
AgroParisTech
2013
Institut Jean-Pierre Bourgin
2013
Bulgarian Academy of Sciences
2007-2012
Abstract Cellular redox homeostasis is a hub for signal integration. Interactions between metabolism and the ABSCISIC ACID-INSENSITIVE-4 (ABI4) transcription factor were characterized in Arabidopsis thaliana vitamin c defective1 (vtc1) vtc2 mutants, which are defective ascorbic acid synthesis show slow growth phenotype together with enhanced abscisic (ABA) levels relative to wild type (Columbia-0). The 75% decrease leaf ascorbate pool mutants was not sufficient adversely affect GA...
In the last decade, microarray studies have delivered extensive inventories of transcriptome-wide changes in messenger RNA levels provoked by various types oxidative stress Arabidopsis (Arabidopsis thaliana). Previous cross-study comparisons indicated how different reactive oxygen species (ROS) and their subcellular accumulation sites are able to reshape transcriptome specific manners. However, these analyses often employed simplistic statistical frameworks that not compatible with...
The genes coding for the core metabolic enzymes of photorespiratory pathway that allows plants with C3-type photosynthesis to survive in an oxygen-rich atmosphere, have been largely discovered genetic screens aimed isolate mutants are unviable under ambient air. As exception, glycolate oxidase (GOX) a phenotype not described yet C3 species. Using Arabidopsis (Arabidopsis thaliana) lacking peroxisomal CATALASE2 (cat2-2) display stunted growth and cell death lesions air, we isolated...
Abstract Halophytes display distinctive physiological mechanisms that enable their survival and growth under extreme saline conditions. This makes them potential candidates for use in agriculture. In this research, tomato ( Solanum lycopersium Mill.) was cultivated moderately conditions two different managements involving Arthrocaulon macrostachyum L., a salt accumulator shrub: intercropping, i.e. , co‐cultivation of tomato/halophyte; crop rotation, which is grown where the halophyte...
Aims: Aphids, like other insects, are probably unable to synthesize vitamin C (ascorbic acid), which is therefore an essential dietary nutrient that has be obtained from the host plant. Plant responses aphids involve hormones such as salicylic acid (SA), jasmonic (JA), and abscisic (ABA), but hormone/redox interactions remain poorly characterized. We investigated signaling in response of Arabidopsis thaliana infestation by aphid Myzus persicae, focusing on between ascorbic ABA, together with...
The high metabolic flux through photorespiration constitutes a significant part of the carbon cycle. Although major enzymatic steps photorespiratory pathway are well characterized, little information is available on functional significance beyond recycling. Particularly important in this respect peroxisomal catalase activity which removes H2O2 generated during oxidation glycolate to glyoxylate, thus maintaining cellular redox homeostasis governing perception, integration and execution stress...
The peach-potato aphid (Myzus persicae Sulzer) is a major pest of potato (Solanum tuberosum L.) but the molecular characterization this interaction particularly with regard to oxidants and antioxidants remains be undertaken. Aphid colonies reared on leaves containing high ascorbate were twice size those grown low ascorbate. Infestation-dependent decreases in abundance key transcripts such as chloroplastic FeSOD, peroxisomal catalase 2, PR1 JAZ1 preceded detectable leaf H(2)O(2) or polyphenol...
Hydrogen peroxide (H2O2) can act as a signaling molecule that influences various aspects of plant growth and development, including stress cell death. To analyze molecular mechanisms regulate the response to increased H2O2 levels in cells, we focused on photorespiration-dependent peroxisomal production Arabidopsis thaliana mutants lacking CATALASE2 (CAT2) activity (cat2-2). By screening for second-site mutations attenuate PSII maximum efficiency (Fv'/Fm') decrease lesion formation linked...
The cellular reduction-oxidation (redox) hub processes information from metabolism and the environment so regulates plant growth defense through integration with hormone signaling network. One key pathway of redox control involves interactions ABSCISIC ACID (ABA). Accumulating evidence suggests that ABA-INSENSITIVE-4 (ABI4) transcription factor plays a role in transmitting concerning abundance ascorbate hence ability cells to buffer oxidative challenges. ABI4 is required for...
The complex process of phloem sugar transport involves symplasmic and apoplasmic events. We characterized Arabidopsis thaliana lines ectopically expressing a phloem-specific gene encoding NDR1/HIN1-like26 (NHL26), putative membrane protein. NHL26 overexpressor plants grew more slowly than wild-type plants, accumulated high levels carbohydrates in mature leaves, had higher shoot biomass, contrasting with slower root growth lower seed yield. Similar effects were observed when was overexpressed...
The responses of C(3) plants to rising atmospheric CO(2) levels are considered be largely dependent on effects exerted through altered photosynthesis. In contrast, the nature C(4) high remains controversial because absence -dependent this study, availability transcriptome, proteome and metabolome profiles two ranks source leaves in maize (Zea mays L.) were studied grown under ambient conditions (350 +/- 20 µL L(-1) ) or with enrichment (700 ). Growth at had no effect photosynthesis,...
Phytophthora cinnamomi is one of the most invasive tree pathogens that devastates wild and cultivated forests. Due to its wide host range, knowledge infection process at molecular level lacking for hosts. To expand repertoire studied Phytophthora–woody plant interactions identify mechanisms can facilitate discovery novel ways control spread damaging effects, we focused on interaction between P. sweet chestnut (Castanea sativa), an economically important wood processing industry. By using a...
Abstract In aerobic life forms, reactive oxygen species (ROS) are produced by the partial reduction of during energy-generating metabolic processes. plants, ROS production increases periods both abiotic and biotic stress, severely overloading antioxidant systems. Hydrogen peroxide (H2O2) plays a central role in cellular redox homeostasis signaling oxidising crucial cysteines to sulfenic acid, which is considered biologically relevant post-translational modification (PTM). Until now, impact...
The influence of extraction medium and sample preparation on the total antioxidant capacity (TAC) pea plants leaves is investigated. Sodium acetate buffer (pH 5), potassium phosphate 7.5), methanol or 0.1% w/v trichloroacetic acid (TCA) were used for homogenization. TAC was measured in crude supernatants (one-step procedure). pellets extracted with acetone their values added to those from previous assay (two-step extract, low-molecular protein fractions derived a partial fractionation probes...
Phytophthora cinnamomi Rands is a cosmopolite pathogen of woody plants which during the last couple centuries has spread all over world from its center origin in Southeast Asia. In contrast to Chinese cork oak (Quercus variabilis Blume) forests native Asia, are generally healthy despite presence pathogen, populations Cork oaks suber L.) Europe have been severely decimated by P. cinnamomi. The present study aims at identifying differences early proteomic and metabolomic response these two...
The phytohormone cytokinin is implicated in a range of growth, developmental, and defense processes. A growing body evidence supports crosstalk between nutrient signaling pathways, such as nitrate availability. Cytokinin regulates sulfur-responsive gene expression, but the underlying molecular mechanisms their impact on sulfur-containing metabolites have not been systematically explored. Using combination genetic pharmacological tools, we investigated interplay sulfur homeostasis. Exogenous...