A5014536168- Cellular transport and secretion
- Plant Molecular Biology Research
- Polysaccharides and Plant Cell Walls
- Plant Reproductive Biology
- Plant tissue culture and regeneration
- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Plant nutrient uptake and metabolism
- Lipid Membrane Structure and Behavior
- Calcium signaling and nucleotide metabolism
- Plant Gene Expression Analysis
- Listeria monocytogenes in Food Safety
- Wnt/β-catenin signaling in development and cancer
- Phytochemistry and biological activities of Ficus species
- Greenhouse Technology and Climate Control
- Plant biochemistry and biosynthesis
- Plant Virus Research Studies
- Glycosylation and Glycoproteins Research
- Skin and Cellular Biology Research
- Botanical Research and Chemistry
- Escherichia coli research studies
- Food Safety and Hygiene
- Lipid metabolism and biosynthesis
- Viral gastroenteritis research and epidemiology
- Biochemical and Structural Characterization
Scribe Biosciences (United States)
2025
Ghent University
2002-2022
VIB-UGent Center for Plant Systems Biology
2013-2022
University of California, Riverside
2018-2021
Plant thirst quenched without water Drought causes many billions of dollars annual losses to farmers worldwide. Central a plant's use efficiency are signaling pathways regulated by the hormone abscisic acid and its receptors. Vaidya et al. screened pool candidate small molecules used structure-guided design optimize function an receptor agonist (see Perspective Phillips Sussman). Application protected Arabidopsis , wheat, tomato from underwatering. Science this issue p. eaaw8848 ; see also 416
Abstract Clathrin-mediated endocytosis (CME) regulates many aspects of plant development, including hormone signaling and responses to environmental stresses. Despite the importance this process, machinery that CME in plants is largely unknown. In mammals, heterotetrameric ADAPTOR PROTEIN COMPLEX-2 (AP-2) required for formation clathrin-coated vesicles at plasma membrane (PM). Although existence AP-2 has been predicted Arabidopsis thaliana, biochemistry functionality complex still...
The Arabidopsis thaliana endogenous elicitor peptides (AtPeps) are released into the apoplast after cellular damage caused by pathogens or wounding to induce innate immunity direct binding membrane-localized leucine-rich repeat receptor kinases, PEP RECEPTOR1 (PEPR1) and PEPR2. Although PEPR-mediated signaling components responses have been studied extensively, contributions of subcellular localization dynamics active PEPRs remain largely unknown. We used live-cell imaging fluorescently...
Abstract ATP production requires the establishment of an electrochemical proton gradient across inner mitochondrial membrane. Mitochondrial uncouplers dissipate this and disrupt numerous cellular processes, including vesicular trafficking, mainly through energy depletion. Here we show that Endosidin9 (ES9), a novel uncoupler, is potent inhibitor clathrin-mediated endocytosis (CME) in different systems ES9 induces inhibition CME not because its effect on ATP, but rather due to protonophore...
SignificanceChemical genetics, which investigates biological processes using small molecules, is gaining interest in plant research. However, a major challenge to uncover the mode of action molecules. Here, we applied cellular thermal shift assay coupled with mass spectrometry (CETSA MS) intact Arabidopsis cells and showed that bikinin, plant-specific glycogen synthase kinase 3 (GSK3) inhibitor, changed stability some its direct targets putative GSK3-interacting proteins. In combination...
Significance Abscisic acid (ABA) is a phytohormone that plants utilize to coordinate responses abiotic stress, modulate seed dormancy, and central plant development in several contexts. Chemicals activate or block ABA signaling are useful as research tools potential agrochemical leads. Many successes have been reported for activators (agonists), but existing blockers (antagonists) limited by modest vivo activity. Here we report antabactin (ANT), potent blocker developed using “click...
For several decades, recurring outbreaks of human gastrointestinal infections associated with contaminated sprouts have posed an enduring challenge, highlighting the necessity controlling etiological agents on sprout seeds. This study investigated efficacy calcium hypochlorite and peroxyacetic acid treatments in inactivating cells four enterohemorrhagic Escherichia coli (EHEC) isolates—viz. E. O157:H7 K4492, F4546, H1730, as well O104:H4 BAA-2326—on alfalfa seeds sprouts. The 2–3 log CFU/g...
Small GTP-binding proteins from the ADP-ribosylation factor (ARF) family are important regulators of vesicle formation and cellular trafficking in all eukaryotes. ARF activation is accomplished by a protein guanine nucleotide exchange factors (GEFs) that contain conserved catalytic Sec7 domain. Here, we identified characterized Secdin, small-molecule inhibitor Arabidopsis thaliana ARF-GEFs. Secdin application caused aberrant retention plasma membrane (PM) late endosomal compartments,...
Several small-molecule perturbagens of the plant endomembrane system are known, but few selectively disrupt endoplasmic reticulum (ER) structure and function. We conducted a microscopy-based screen for disruptors ER discovered eroonazole, 1,2-4-triazole that induces extensive vesiculation in Arabidopsis seedlings. To identify eroonazole targets, we synthesized clickable photoaffinity derivative used it whole-seedling labeling experiments. These reveal probe labels multiple oleosins, membrane...
Attempts were made to isolate 3-chloroaniline (3-CA) and 3,4-dichloroaniline (3,4-DCA) degrading bacteria from the A- B-horizon of three different soils. A variety 3-CA was obtained all soils, whereas 3,4-DCA strains only isolated one soil. Amongst degraders, two belong γ-Proteobacteria seven β-Proteobacteria. Of latter group, five are members family Comamonadaceae. Interestingly, isolates contained an IncP-1β plasmid. These plasmids could be divided into four major groups based on...
Abstract Despite the growing interest in using chemical genetics plant research, small-molecule target identification remains a major challenge. The cellular thermal shift assay coupled with high-resolution mass-spectrometry (CETSA MS) that monitors changes stability of proteins caused by their interactions small molecules, other proteins, or post-translational modifications allows drug targets, study protein-metabolite and protein-protein mainly mammalian cells. To showcase applicability...