A5020065657- Nematode management and characterization studies
- Plant-Microbe Interactions and Immunity
- Advanced Glycation End Products research
- Plant Stress Responses and Tolerance
- Natural Products and Biological Research
- Legume Nitrogen Fixing Symbiosis
- Sugarcane Cultivation and Processing
- Plant nutrient uptake and metabolism
- Plant Molecular Biology Research
- Phytochemicals and Antioxidant Activities
- Plant tissue culture and regeneration
- Listeria monocytogenes in Food Safety
- Plant Parasitism and Resistance
- Agronomic Practices and Intercropping Systems
- Agriculture, Plant Science, Crop Management
- Aluminum toxicity and tolerance in plants and animals
- Inflammatory mediators and NSAID effects
- Seed and Plant Biochemistry
- Fungal and yeast genetics research
- Plant pathogens and resistance mechanisms
- Bioinformatics and Genomic Networks
- Plant Pathogenic Bacteria Studies
- Antimicrobial agents and applications
- Diverse Scientific Research Studies
- Food composition and properties
Scribe Biosciences (United States)
2023-2025
Cornell University
2014-2024
Ithaca College
2015-2024
Russian Research Institute for Phytopathology
2024
Baylor College of Medicine
2010-2017
University of Mysore
2008-2014
Agricultural Research Service
2010-2011
Texas A&M University
2010-2011
Children's Nutrition Research Center at Baylor College of Medicine
2010-2011
United States Department of Agriculture
2010-2011
Abstract Plant-defense responses are triggered by perception of conserved microbe-associated molecular patterns (MAMPs), for example, flagellin or peptidoglycan. However, it remained unknown whether plants can detect derived from plant-parasitic animals, including nematodes. Here we show that several genera nematodes produce small molecules called ascarosides, an evolutionarily family nematode pheromones. Picomolar to micromolar concentrations ascr#18, the major ascaroside in nematodes,...
Salicylic acid (SA) is an important hormone involved in many diverse plant processes, including floral induction, stomatal closure, seed germination, adventitious root initiation, and thermogenesis. It also plays critical functions during responses to abiotic biotic stresses. The role(s) of SA signaling disease resistance by far the best studied process, although it still only partially understood. To obtain insights into how carries out its varied functions, particularly activating...
Salicylic acid (SA) and its derivatives have been used for millennia to reduce pain, fever inflammation. In addition, prophylactic use of acetylsalicylic acid, commonly known as aspirin, reduces the risk heart attack, stroke certain cancers. Because aspirin is rapidly de-acetylated by esterases in human plasma, much aspirin's bioactivity can be attributed primary metabolite, SA. Here we demonstrate that high mobility group box 1 (HMGB1) a novel SA-binding protein. sites on HMGB1 were...
Abstract Microorganisms and nematodes in the rhizosphere profoundly impact plant health, small-molecule signaling is presumed to play a central role interactions. However, nature of signals underlying mechanisms are poorly understood. Here we show that ascaroside ascr#18, pheromone secreted by plant-parasitic nematodes, metabolized plants generate chemical repel reduce infection. Comparative metabolomics tissues excretions revealed ascr#18 converted into shorter side-chained ascarosides...
Damage-associated molecular pattern molecules (DAMPs) signal the presence of tissue damage to induce immune responses in plants and animals. Here, we report that High Mobility Group Box 3 (HMGB3) is a novel plant DAMP. Extracellular HMGB3, through receptor-like kinases BAK1 BKK1, induced hallmark innate responses, including i) MAPK activation, ii) defense-related gene expression, iii) callose deposition, iv) enhanced resistance Botrytis cinerea. Infection by necrotrophic B. cinerea released...
The plant hormone salicylic acid (SA) controls several physiological processes and is a key regulator of multiple levels immunity. To decipher the mechanisms through which SA's effects are mediated, particularly in immunity, two high-throughput screens were developed to identify SA-binding proteins (SABPs). Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) from plants (Arabidopsis thaliana) was identified these screens. Similar subsequent analyses using SA analogs, conjunction with either...
The interplay between abscisic acid (ABA) and salicylic (SA) influences plant responses to various (a)biotic stresses; however, the underlying mechanism for this crosstalk is largely unknown. Here, we report that type 2C protein phosphatases (PP2Cs), some of which are negative regulators ABA signaling, bind SA. SA binding suppressed ABA-enhanced interaction these PP2Cs receptors belonging PYR/PYL/RCAR family. Additionally, degradation ABA-induced stabilization SnRK2s. Supporting SA's role as...
Arabidopsis thaliana cation exchangers (CAX1 and CAX3) are closely related tonoplast-localized calcium/proton (Ca2+/H+) antiporters that contribute to cellular Ca2+ homeostasis. CAX1 CAX3 were previously shown interact in yeast; however, the function of this complex plants has remained elusive. Here, we demonstrate expression occurs guard cells. Additionally, co-expressed mesophyll tissue response wounding or flg22 treatment, due induction expression. Having transporters can be same cells,...
For several decades, recurring outbreaks of human gastrointestinal infections associated with contaminated sprouts have posed an enduring challenge, highlighting the necessity controlling etiological agents on sprout seeds. This study investigated efficacy calcium hypochlorite and peroxyacetic acid treatments in inactivating cells four enterohemorrhagic Escherichia coli (EHEC) isolates—viz. E. O157:H7 K4492, F4546, H1730, as well O104:H4 BAA-2326—on alfalfa seeds sprouts. The 2–3 log CFU/g...
Plant calcium (Ca2+) gradients, millimolar levels in the vacuole and micromolar cytoplasm, are regulated part by high-capacity vacuolar cation/H+ exchangers (CAXs). Several CAX transporters, including CAX1, appear to contain an approximately 40-amino acid N-terminal regulatory region (NRR) that modulates transport through autoinhibition. Deletion of NRR from several CAXs (sCAX) enhances function plant yeast expression assays; however, date, there no functional assays for CAX3 (or sCAX3),...
Abstract Recognition of specific molecule signatures microbes, including pathogens, induces innate immune responses in plants, as well animals. Analogously, a nematode pheromone, the ascaroside ascr#18, hallmark plant defences activation (a) mitogen‐activated protein kinases, (b) salicylic acid‐ and jasmonic acid‐mediated defence signalling pathways (c) gene expression provides protection to broad spectrum pathogens. Ascr#18 is member an evolutionarily conserved family molecules major...
To elucidate one or more mechanisms through which microrchidia (MORC) proteins impact immunity, epigenetic gene silencing, and DNA modifications, the enzymatic activities of plant MORCs were characterized. Previously, we showed that MORC1s have ATPase endonuclease activities. Here, demonstrate topoisomerase type II (topo II)-like activities, as they i) covalently bind DNA, ii) exhibit DNA-stimulated activity, iii) relax nick supercoiled iv) catenate v) decatenante kinetoplast DNA. Mutational...
Many bacterivorous and parasitic nematodes secrete signaling molecules called ascarosides that play a central role regulating their behavior development. Combining stable-isotope labeling mass spectrometry-based comparative metabolomics, here we show are taken up from the environment metabolized by wide range of phyla, including plants, fungi, bacteria, mammals, as well nematodes. In most tested eukaryotes some into derivatives with shortened fatty acid side chains, analogous to ascaroside...
MORC1 and MORC2, two of the seven members Arabidopsis (Arabidopsis thaliana) Compromised Recognition Turnip Crinkle Virus1 subfamily microrchidia Gyrase, Heat Shock Protein90, Histidine Kinase, MutL (GHKL) ATPases, were previously shown to be required in multiple layers plant immunity. Here, we show that barley (Hordeum vulgare) MORCs also are involved disease resistance. Genome-wide analyses identified five 37% 48% identical on protein level AtMORC1. Unexpectedly, clear contrast...
CAtion/H(+) eXchangers (CAXs) are integral membrane proteins that transport Ca(2+) or other cations by exchange with protons. While several yeast and plant CAX have been characterized, no functional analysis of a vertebrate homologue has yet reported. In this study, we further characterize from yeast, VNX1, initiate characterization zebrafish (Cax1). Localization studies indicated both Vnx1 Cax1 found in endomembrane compartments. Biochemical fractions vnx1 mutant cells Cax1-expressing...
The biochemical properties and regulation of several plant CAX (CAtion eXchanger)-type vacuolar Ca2+/H+ exchangers have been extensively analysed in yeast expression assays. In the present study, we compare contrast phenotypes cells expressing CAX1 cDNA open reading frame (ORF). We report that ORF, but not containing 3′-untranslated region (UTR), was able to confer Ca2+ tolerance when expressed a Ca2+-sensitive mutant. Additionally, only yeasts N-terminal truncated ORF were grow on high Mn2+...
The microrchidia (MORC) proteins, a subset of the GHKL ATPase superfamily, were recently described as components involved in transcriptional gene silencing and plant immunity Arabidopsis. To assess role MORC1 during resistance to Phytophthora infestans solanaceous species, we altered expression corresponding homologs potato, tomato, Nicotiana benthamiana. Basal P. was compromised StMORC1-silenced potato enhanced overexpressing lines, indicating that StMORC1 positively affects immunity. By...
A novel, natural, and effective antimicrobial intervention is in demand for improving the microbial safety of vegetable seeds/sprouts. This study assessed efficacy ascaroside treatment control Salmonella enterica on alfalfa fenugreek sprouts. Sanitized commercial seeds were treated with 1 mM or µM (ascr)#18, a plant immunity modulator (PIM) dried an hour before being inoculated lyophilized S. Cubana Stanley cells sandy soil (104 CFU/g). Treated untreated spouted 1% water agar at 25°C dark....
<title>Abstract</title> Leaf rust, caused by the pathogenic biotrophic rust fungus <italic>Puccinia triticina</italic> (<italic>Pt</italic>), is one of most destructive wheat diseases worldwide; its negative impact on crop yields exacerbated increasing temperatures due to climate change. Ascarosides are nematode pheromones that induce resistance microbial pathogens and pests in a wide range crops, making them valuable components biocontrol scenarios. We investigated effect infection various...