A5086808449- Cancer, Hypoxia, and Metabolism
- Renal cell carcinoma treatment
- Cancer, Lipids, and Metabolism
- Epigenetics and DNA Methylation
- Hormonal Regulation and Hypertension
- Renal and related cancers
- Ferroptosis and cancer prognosis
- RNA modifications and cancer
- RNA Research and Splicing
- Cancer Genomics and Diagnostics
- Amino Acid Enzymes and Metabolism
- Metabolism, Diabetes, and Cancer
- Diet and metabolism studies
- Cell death mechanisms and regulation
- Cancer-related molecular mechanisms research
- Cancer-related gene regulation
- PI3K/AKT/mTOR signaling in cancer
- Adrenal and Paraganglionic Tumors
- Glutathione Transferases and Polymorphisms
- Cancer Research and Treatments
- Bladder and Urothelial Cancer Treatments
- Genetic and Kidney Cyst Diseases
- DNA Repair Mechanisms
- Kruppel-like factors research
- Metalloenzymes and iron-sulfur proteins
University of Alabama at Birmingham
2014-2024
American University of Antigua
2023
Melaka Manipal Medical College
2022
Manipal Academy of Higher Education
2022
Birmingham VA Medical Center
2017-2021
O'Neal Comprehensive Cancer Center
2020-2021
Karnataka Veterinary Animal and Fisheries Sciences University
2019-2021
University of Alabama at Birmingham Hospital
2016
St. Vincent's Birmingham
2013-2014
University of Mysore
2014
Abstract Through unbiased metabolomics, we identified elevations of the metabolite 2-hydroxyglutarate (2HG) in renal cell carcinoma (RCC). 2HG can inhibit 2-oxoglutaratre (2-OG)–dependent dioxygenases that mediate epigenetic events, including DNA and histone demethylation. accumulation, specifically d enantiomer, result from gain-of-function mutations isocitrate dehydrogenase (IDH1, IDH2) found several different tumors. In contrast, kidney tumors demonstrate l enantiomer (l-2HG). High-2HG...
Hereditary leiomyomatosis and renal cell cancer (HLRCC) is an inherited syndrome linked to biallelic inactivation of the gene encoding tricarboxylic acid cycle enzyme fumarate hydratase (FH). Individuals with HLRCC are at risk develop cutaneous uterine leiomyomas aggressive form kidney cancer. Pseudohypoxic drive-the aberrant activation cellular hypoxia response pathways despite normal oxygen tension-is considered be a likely mechanism underlying etiology this tumor. Pseudohypoxia requires...
No AccessJournal of UrologyAdult Urology1 Jul 2008Feasibility and Outcomes Repeat Partial Nephrectomyis accompanied byCpG Hypermethylation the UCHL1 Gene Promoter is Associated With Pathogenesis Poor Prognosis in Renal Cell CarcinomaAntisense Attenuation p21 Sensitizes Kidney Cancer to Apoptosis Response Conventional DNA Damaging Chemotherapy Enhancement Phospho-p53A Pilot Trial Vascular Targeted Photodynamic Therapy for Tissue Aaron Johnson, Sunil Sudarshan, Jack Liu, W. Marston Linehan,...
Background Inflammatory cytokines are detected in the plasma of patients with renal cell carcinoma (RCC) and associated poor prognosis. However, primary type involved producing inflammatory biological significance RCC remain unknown. Inflammation is oxidative stress, upregulation hypoxia inducible factor 1-alpha, production pro-inflammatory gene products. Solid tumors often heterogeneous oxygen tension together suggesting that may play a role processes RCC. Epithelial cells have been...
// Arindam P. Ghosh 1 , Christopher B. Marshall 4 Tatjana Coric 2 Eun-hee Shim Richard Kirkman Mary E. Ballestas 3 Mitsuhiko Ikura Mary-Ann Bjornsti and Sunil Sudarshan Department of Urology, University Alabama at Birmingham, Alabama, USA Pharmacology Toxicology, Pediatrics, Medical Biophysics, Campbell Family Cancer Research Institute, Princess Margaret Centre, Toronto, Canada Correspondence to: Sudarshan, email: Keywords : mTOR, RHEB, rapamycin, renal cancer, mutations Received June 08,...
Elevation of L-2-hydroxylgutarate (L-2-HG) in renal cell carcinoma (RCC) is due part to reduced expression L-2-HG dehydrogenase (L2HGDH). However, the contribution carcinogenesis and insight into biochemistry targets this small molecule remains be elucidated.Genetic pharmacologic approaches modulate levels were assessed for effects on vitro vivo phenotypes. Metabolomics was used dissect biochemical mechanisms that promote accumulation RCC cells. Transcriptomic analysis utilized identify...
Patients with von Hippel-Lindau disease may require repeat partial nephrectomies to avoid the need for renal replacement therapy. We evaluated outcomes in patients who underwent third or fourth on same kidney, which we call salvage nephrectomy.We retrospectively reviewed charts of surgery at National Cancer Institute from 1999 2006. identified 11 13 whom nephrectomy was performed. Surgical were assessed by preservation as well intraoperative and postoperative complications. Functional...
We examined outcomes in patients with recurrent or de novo renal lesions treated repeat partial nephrectomy on a solitary kidney.We reviewed the records of who underwent nephron sparing surgery at National Cancer Institute from 1989 to 2008. Patients were included analysis if they kidney. Perioperative, functional and oncological assessed. Functional evaluated using Modification Diet Renal Disease equation for estimated glomerular filtration rate. Oncological efficacy was by need subsequent...
Inactivating mutations of the gene encoding tricarboxylic acid cycle enzyme fumarate hydratase (FH) have been linked to an aggressive variant hereditary kidney cancer (hereditary leiomyomatosis and renal cell cancer). These tumors accumulate markedly elevated levels fumarate. Fumarate is among a growing list oncometabolites identified in cancers with genes involved intermediary metabolism. FH-deficient are notable for their pronounced accumulation transcription factor hypoxia inducible...
Analysis of transcriptomic data demonstrates extensive epigenetic gene silencing the transcription factor PRDM16 in renal cancer. We show that restoration RCC cells suppresses vivo tumor growth. RNaseq analysis reveals imparts a predominantly repressive effect on transcriptome including suppression encoding semaphorin 5B (SEMA5B). SEMA5B is HIF target highly expressed promotes Functional studies demonstrate PRDM16's properties, mediated by physical interaction with transcriptional...
Abstract Pseudouridylation is a common RNA modification that catalyzed by the family of pseudouridine synthases (PUS). can increase stability and rigidity, thereby impacting splicing, processing, translation. Given metabolism frequently altered in cancer, pseudouridylation may be functionally important process tumor biology. Here, we showed MYC oncoproteins transcriptionally upregulates PUS7 expression during cancer development. was essential for growth survival MYC-driven cells xenografts...
The Cancer Genome Atlas (TCGA) and other large-scale genomic data pipelines have been integral to the current understanding of molecular events underlying renal cell carcinoma (RCC). These networks focused mostly on primary RCC, which often demonstrates indolent behavior. However, metastatic disease is major cause mortality associated with RCC sets examining tumors are sparse. Therefore, a more comprehensive analysis gene expression DNA methylome profiling in addition normal kidney was...
The pyruvate aldolases use as the nucleophilic component in stereoselective aldol condensations, producing a 4-hydroxy-2-ketobutyrate framework. We have examined 2-keto-3-deoxy-6-phosphogluconate (KDPG) from Pseudomonas putida, Escherichia coli, and Zymomonas mobilis for utility synthetic reagents. Unlike other to date, KDPG accept short-chain, non-carbohydrate electrophilic aldehydes substrates, providing general methodology construction of skeleton. three differ markedly with respect...
Germline mutations of FH, the gene that encodes for tricarboxylic acid TCA (TCA) cycle enzyme fumarate hydratase, are associated with an inherited form cancer referred to as Hereditary Leiomyomatosis and Renal Cell Cancer (HLRCC). Individuals HLRCC predisposed development highly malignant lethal renal cell carcinoma (RCC). The mechanisms tumorigenesis proposed have largely focused on biochemical consequences loss FH enzymatic activity. While tumor suppressor von Hippel Lindau (VHL) is...
Despite the widespread use of kinase-targeted agents in clear cell renal carcinoma (CC-RCC), comprehensive kinase activity evaluation (kinomic profiling) these tumors is lacking. Thus, kinomic profiling CC-RCC may assist devising a classification system associated with clinical outcomes, and help identify potential therapeutic targets. Fresh frozen tumor lysates from 41 clinically annotated patients who had localized disease at diagnosis were kinomically profiled using PamStation®12...