A5054005360- Tissue Engineering and Regenerative Medicine
- Pluripotent Stem Cells Research
- Congenital heart defects research
- CRISPR and Genetic Engineering
- Viral Infectious Diseases and Gene Expression in Insects
- Neuroscience and Neural Engineering
- Pancreatic function and diabetes
- Adipose Tissue and Metabolism
- Cardiac Fibrosis and Remodeling
- Cancer, Hypoxia, and Metabolism
- Protein purification and stability
- Cardiac Ischemia and Reperfusion
- Electrospun Nanofibers in Biomedical Applications
- Hepatitis C virus research
- Plant-Microbe Interactions and Immunity
- RNA modifications and cancer
- Pediatric health and respiratory diseases
- Genetics, Aging, and Longevity in Model Organisms
- 3D Printing in Biomedical Research
- Crystallization and Solubility Studies
- Circadian rhythm and melatonin
- Plant Pathogens and Resistance
- RNA Interference and Gene Delivery
- Dietary Effects on Health
- Extracellular vesicles in disease
Bristol-Myers Squibb (United States)
2024
University of Alabama at Birmingham
2016-2023
Jiangsu Normal University
2023
Rodent hearts can regenerate myocardium lost to apical resection or myocardial infarction for up 7 days after birth, but whether a similar window regeneration also exists in large mammals is unknown.Acute (AMI) was surgically induced neonatal pigs on postnatal 1, 2, 3, 7, and 14 (ie, the P1, P2, P3, P7, P14 groups, respectively). Cardiac systolic function evaluated before AMI at 30 post-AMI via transthoracic echocardiography. Cardiomyocyte cell cycle activity assessed immunostaining...
Abstract Differentiation of cardiomyocytes (CMs) from human induced pluripotent stem cells (hiPSCs) is critically dependent upon the regulation Wnt signaling pathway. The mechanisms remain unclear with regard to dose and timing each differentiation inducer, interaction inducers that regulate in mesendoderm specification cardiac mesoderm. Consequently, it remains far optimal efficiency consistency hiPSC lines CMs. Here, we have carefully deciphered role pathway manipulation on mesoderm a...
Direct cardiac reprogramming of fibroblasts into cardiomyocytes has emerged as a promising strategy to remuscularize injured myocardium. However, it is insufficient generate functional induced from human using conventional cocktails, and the underlying molecular mechanisms are not well studied.To discover potential missing factors for direct reprogramming, we performed transcriptomic comparison between cardiomyocytes.We identified TBX20 (T-box transcription factor 20) top gene that unable be...
Abstract Aims We have shown that human cardiac muscle patches (hCMPs) containing three different types of cells—cardiomyocytes (CMs), smooth cells (SMCs), and endothelial (ECs), all which were differentiated from pluripotent stem (hPSCs)—significantly improved function, infarct size, hypertrophy in a pig model myocardial infarction (MI). However, hPSC-derived CMs (hPSC-CMs) are phenotypically immature, may lead to arrhythmogenic concerns; thus, since fibroblasts (hPSC-CFs) appear enhance the...
Exosomes hold great potential to deliver therapeutic reagents for cancer treatment due its inherent low antigenicity. However, several technical barriers, such as productivity and ineffective targeting, need be overcome before wide clinical applications. The present study aims at creating a new biomanufacturing platform of cancer-targeted exosomes drug delivery. Specifically, scalable, robust, high-yield, cell line based exosome production process is created in stirred-tank bioreactor, an...
Analysis of transcriptomic data demonstrates extensive epigenetic gene silencing the transcription factor PRDM16 in renal cancer. We show that restoration RCC cells suppresses vivo tumor growth. RNaseq analysis reveals imparts a predominantly repressive effect on transcriptome including suppression encoding semaphorin 5B (SEMA5B). SEMA5B is HIF target highly expressed promotes Functional studies demonstrate PRDM16's properties, mediated by physical interaction with transcriptional...
The effectiveness of cell-based treatments for regenerative myocardial therapy is limited by low rates cell engraftment. Y-27632 inhibits Rho-associated protein kinase (ROCK), which regulates the cytoskeletal changes associated with adhesion, and has been used to protect cultured cells during their passaging. Here, we investigated whether preconditioning cardiomyocytes, derived from human-induced pluripotent stem (hiPSC-CM), improves survival engraftment in a murine model acute infarction...
Cardiovascular disease, until now, is still the leading cause of death in United States. Due to limited regenerative capacity adult hearts, damage caused by heart injury cannot be reversed and eventually progress into failure. In need cardiovascular disease treatment, many therapies aimed at either cell transplantation or regeneration have been proposed. Direct reprogramming somatic cells induced cardiomyocytes (iCMs) considered a promising strategy for medicine. The induction from...
Exercise has been shown to be the best intervention in treatment of many diseases. Many benefits exercise are mediated by adaptions induced skeletal muscle. The peroxisome proliferator-activated receptor gamma coactivator-1 (PGC-1) family transcriptional coactivators emerged as being key mediators response and is considered essential for seen However, contribution PGC-1s muscle evaluated use either whole body or congenital muscle-specific deletion. In these models, were never present,...
The mortality of patients suffering from acute myocardial infarction is linearly related to the infarct size. As regeneration cardiomyocytes cardiac progenitor cells minimal in mammalian adult heart, we have explored a new therapeutic approach, which leverages capacity nanomaterials release chemicals over time promote protection and size reduction. Initial screening identified 2 chemicals, FGF1 CHIR99021 (a Wnt1 agonist/GSK-3β antagonist), synergistically enhance cardiomyocyte cell cycle...
The adoptive transfer of human T cells or genetically-engineered with cancer-targeting receptors has shown tremendous promise for eradicating tumors in clinical trials. objective this study was to develop a novel cell biomanufacturing platform using stirred-tank bioreactor large-scale and high-quality cellular production. First, various factors, such as parameters, media, supplements, stimulation, seed age, donors, were investigated. A serum-free fed-batch bioproduction process developed...
The broadly expressed transcriptional coregulator LDB1 is essential for β-cell development and glucose homeostasis. However, it unclear whether has metabolic roles beyond the β-cell, especially under stress. Global Ldb1 deletion results in early embryonic lethality; thus, we used global heterozygous Ldb1+/− inducible β-cell–specific Ldb1-deficient (Ldb1Δβ-cell) mice. We assessed insulin tolerance, body composition, feeding, energy expenditure during high-fat diet exposure. Brown adipose...
A crucial factor in improving cellular therapy effectiveness for myocardial regeneration is to safely and efficiently increase the cell engraftment rate. Y-27632 a highly potent inhibitor of Rho-associated, coiled-coil-containing protein kinase (RhoA/ROCK) used prevent dissociation-induced apoptosis (anoikis). We demonstrate that pretreatment human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs+RI) prior implantation results rate improvement mouse model acute infarction...
Recent optimizations of cell culture processes have focused on the final seed scale-up step (N - 1 stage) used to inoculate production bioreactor (N-stage bioreactor) enable higher inoculation densities (2-20 × 10
Abstract Media preparation parameters contribute significantly to media quality, cell culture performance, productivity, and product quality. Establishing proper procedures is critical for ensuring a robust CHO process. Process analytical technology (PAT) enables unique ways quantify assessments improve Here, were prepared under wide range of temperatures (40–80°C) pH (7.6–10.0). quality profiles compared using three real‐time PATs: Fourier‐transform infrared (FTIR) spectroscopy, Raman...
Abstract The most common genetic aberration associated with inherited or sporadic renal cell carcinoma (RCC) is the loss nonfunctional mutations in von Hippel-Lindau (VHL) tumor suppressor gene. lead to stabilization of hypoxia inducible factor (HIF-1α and 2α), thereby promoting expression HIF target genes. genes are reported induce phenotypes by altering cellular metabolism upregulating protumorigenic/angiogenic factors as well. We report here tissue microarray data histology-based analyses...
Objective: To understand the cognition, willingness to test and influencing factors of hepatitis C (HCV) based on social software for men who have sex with (MSM). Methods: An online questionnaire was used investigate target population Blued platform users, including general demographic characteristics, sexual behavior, drug abuse cognition prevention treatment, past testing status future willingness. There were 14 questions in part, answering 10 or more defined as "knowing" . Cognition rate,...
Direct cardiac reprogramming to generate induced cardiomyocyte like cells (iCMs) from fibroblasts has emerged as a promising therapeutic strategy for the treatment of heart failure, which is still leading cause mortality and morbidity in developed countries. While much known regarding iCMs generated mouse cells, adaptation direct human hurdled with low efficiency poor quality because intrinsic differences between species. Recently, our single cell transcriptomic analysis during demonstrated...