A5058928241- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Tissue Engineering and Regenerative Medicine
- Neuroscience and Neural Engineering
- Congenital heart defects research
- Phosphodiesterase function and regulation
- Synthesis and biological activity
- Electrospun Nanofibers in Biomedical Applications
- Inflammatory mediators and NSAID effects
- 3D Printing in Biomedical Research
- Congenital Heart Disease Studies
- Cholinesterase and Neurodegenerative Diseases
- Chemical synthesis and alkaloids
- Synthesis of β-Lactam Compounds
- Angiogenesis and VEGF in Cancer
- Inflammasome and immune disorders
- Renin-Angiotensin System Studies
- Genomic variations and chromosomal abnormalities
- Heart Rate Variability and Autonomic Control
- Science, Research, and Medicine
- Pleural and Pulmonary Diseases
- Viral Infections and Vectors
- Phagocytosis and Immune Regulation
- SARS-CoV-2 and COVID-19 Research
- Virology and Viral Diseases
Mattapally Technologies
2023
University of Alabama at Birmingham
2017-2021
Indian Institute of Chemical Technology
2012-2018
Fudan University
2017
Academy of Scientific and Innovative Research
2017
Institut thématique Génétique, génomique et bioinformatique
2011
Background: Here, we generated human cardiac muscle patches (hCMPs) of clinically relevant dimensions (4 cm × 2 1.25 mm) by suspending cardiomyocytes, smooth cells, and endothelial cells that had been differentiated from induced-pluripotent stem in a fibrin scaffold then culturing the construct on dynamic (rocking) platform. Methods: In vitro assessments hCMPs suggest maturation response to culture stimulation. vivo were conducted porcine model myocardial infarction (MI). Animal groups...
Background We aim to generate a line of "universal donor" human induced pluripotent stem cells (hi PSC s) that are nonimmunogenic and, therefore, can be used derive cell products suitable for allogeneic transplantation. Methods and Results hi s carrying knockout mutations 2 key components (β2 microglobulin class II major histocompatibility transactivator) complexes I (ie, leukocyte antigen [HLA] I/ were generated using the Clustered Regularly Interspaced Short Palindromic Repeats...
Rationale: The effectiveness of transplanted, human induced pluripotent stem cell–derived cardiomyocytes (hiPSC-CMs) for treatment ischemic myocardial injury is limited by the exceptionally low engraftment rate. Objective: To determine whether overexpression cell cycle activator CCND2 (cyclin D2) in hiPSC-CMs can increase graft size and improve recovery a mouse model infarction increasing proliferation grafted cells. Methods Results: Human was delivered to hiPSCs via lentiviral-mediated gene...
We have previously reported that increased glucose levels were associated with higher serum nitric oxide (NO) in fructose-fed insulin resistant rats. However, the relationship between hyperglycemia and NO level was not clear. Therefore, present study designed to find association Type 2 diabetic (T2DM) patients T2DM cardiovascular complication.Endothelial cells (HUVEC) treated of D-glucose (10-100mM), NOS gene expression measured. Hyperglycaemia induced Sprague-Dawley rats, measured after 8...
The microenvironment of native heart tissue may be better replicated when cardiomyocytes are cultured in three-dimensional clusters (i.e., spheroids) than monolayers or as individual cells. Thus, we differentiated human cardiac lineage-induced pluripotent stem cells (hiPSC-CMs) and allowed them to form spheroids spheroid fusions that were characterized vitro evaluated mice after experimentally induced myocardial infarction (MI). Synchronized contractions observed within 24 h formation,...
Angiogenesis, a process involving the growth of new blood vessels from pre-existing vasculature, plays crucial role in various pathophysiological conditions. We have previously demonstrated that europium hydroxide [EuIII(OH)3] nanorods (EHNs) exhibit pro-angiogenic properties through generation reactive oxygen species (ROS) and mitogen activated protein kinase (MAPK) activation. Considering enormous implication angiogenesis cardiovascular diseases (CVDs) cancer, it is essential to understand...
The effectiveness of cell-based treatments for regenerative myocardial therapy is limited by low rates cell engraftment. Y-27632 inhibits Rho-associated protein kinase (ROCK), which regulates the cytoskeletal changes associated with adhesion, and has been used to protect cultured cells during their passaging. Here, we investigated whether preconditioning cardiomyocytes, derived from human-induced pluripotent stem (hiPSC-CM), improves survival engraftment in a murine model acute infarction...
NKX2.5, a homeobox containing gene, plays an important role in embryonic heart development and associated mutations are linked with various cardiac abnormalities. We sequenced the NKX2.5 gene 100 congenital disease (CHD) patients 200 controls. Our analysis revealed total of 7 mutations, 3 intronic region, coding region 1 3' UTR. Of above one mutation was found to be tetralogy fallot (TOF) two (rs2277923 novel mutation, D16N) were strongly VSD. A missense D16N (p-value =0.009744), located...
Congenital heart diseases (CHDs) usually refer to abnormalities in the structure and/or function of that arise before birth. GATA4 plays an important role embryonic development, hence aim this study was find association mutations with CHD among south Indian patients.GATA4 gene sequenced 100 patients (ASD, VSD, TOF and SV) 200 controls. Functional significance observed analyzed using PolyPhen, SIFT, PMut, Plink, Haploview, ESE finder 3.0 CONSITE.We a total 19 mutations, which, one 5' UTR, 10...
The role of p53 transactivation domain (p53-TAD), a multifunctional and dynamic domain, on DNA repair retaining integrity in human induced pluripotent stem cells (hiPSCs) has never been studied. p53-TAD was knocked out iPSCs using CRISPR/Cas9 confirmed by sequencing. knockout (KO) were characterized accelerated proliferation, decreased population doubling time, unaltered Bcl-2, Bcl-2-binding component 3, insulin-like growth factor 1 receptor, Bax altered Mdm2, p21, p53-induced death...
As a zoonotic virus, the nipah virus (NiV) can pass from people to animals. A novel paramyxovirus called NiV, which is related Hendra and has spread globally, had tremendous impact on people's life. Numerous investigations have shown that NiV SARS-CoV, was responsible for 2002 outbreak of severe acute respiratory syndrome, lot in common biologically. While alone also cause myocardial damage, pre-existing cardiovascular illness appears be associated with worse results an increased risk death...
Introduction: Human induced pluripotent stem cells (hiPSCs) are among one the most significant discoveries in life sciences. As a promising —biological drug for cell therapy, multiple lineages of iPSC-derived cardiac have been administered human clinical trials important organ systems. The potential immunogenicity hiPSC-derived continues to be concerns large animal models. Methods: In present study, WT hiPSCs were generated by transfecting male fibroblasts with Sendai viruses coding OCT4,...
Abstract 20 Compounds of type (VI) are prepared and screened for their COX‐1 COX‐2 inhibitory activities.
Transplantation of cardiomyocytes derived from induced pluripotent stem cells (iPSCs) improves cardiac function in animal models with myocardial infarction. However, the poor number survived and low proliferation capability cardiomyocyte iPSCs are bottlenecks repair cell therapy. We hypothesize that increasing surviving iPSC-CMs engraftment via cycle induction may lead to a transmural replacement scar tissue lasting restoration function. Cyclin D2 is protein regulates transition G1 S phase....
Introduction: Human induced pluripotent stem cells (hiPSCs) have the ability to produce a huge variety of terminally differentiated cell types that can be used for tissue engineering, drug research, and therapy. Using CRISPR/Cas9 gene-editing technologies, hiPSCs with knockout mutations Per1, Per2, CIITA, B2M, N-Cadherin, Connexin43 over expression were created. Cardiomyocytes (CM) created from hiPSCs, spheroid culture was carried out as previously described. The molecular basis circadian...
Abstract 26 Pyridone derivatives of type (IV) are prepared and tested as inhibitors phosphodiesterase3 (PDE3).