A5008609963- Cancer-related molecular mechanisms research
- Liver Disease Diagnosis and Treatment
- Adipose Tissue and Metabolism
- RNA modifications and cancer
- Peroxisome Proliferator-Activated Receptors
- Tissue Engineering and Regenerative Medicine
- MicroRNA in disease regulation
- Mitochondrial Function and Pathology
- Pluripotent Stem Cells Research
- Extracellular vesicles in disease
- Endoplasmic Reticulum Stress and Disease
- Lipid metabolism and disorders
- RNA Research and Splicing
- Metabolism, Diabetes, and Cancer
- Heat shock proteins research
- Cardiac Fibrosis and Remodeling
- Cholesterol and Lipid Metabolism
- Estrogen and related hormone effects
- Circular RNAs in diseases
- Reconstructive Surgery and Microvascular Techniques
- Genetics, Aging, and Longevity in Model Organisms
- CRISPR and Genetic Engineering
- Lipid metabolism and biosynthesis
- Congenital heart defects research
- 3D Printing in Biomedical Research
University of Alabama at Birmingham
2019-2024
Chinese Academy of Sciences
2022-2024
Shanghai Institute of Materia Medica
2022-2024
Academia Sinica
2024
University of Chinese Academy of Sciences
2024
Wenzhou Medical University
2021
Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University
2021
Nanyang Normal University
2021
Augusta University
2017-2018
Augusta University Health
2017-2018
Abstract Aims We have shown that human cardiac muscle patches (hCMPs) containing three different types of cells—cardiomyocytes (CMs), smooth cells (SMCs), and endothelial (ECs), all which were differentiated from pluripotent stem (hPSCs)—significantly improved function, infarct size, hypertrophy in a pig model myocardial infarction (MI). However, hPSC-derived CMs (hPSC-CMs) are phenotypically immature, may lead to arrhythmogenic concerns; thus, since fibroblasts (hPSC-CFs) appear enhance the...
Rationale:The conserved long non-coding RNA (lncRNA) myocardial infarction associate transcript (Miat) was identified for its multiple single-nucleotide polymorphisms that are strongly associated with susceptibility to MI, but role in cardiovascular biology remains elusive.Here we investigated whether Miat regulates cardiac response pathological hypertrophic stimuli.Methods: Both an angiotensin II (Ang II) infusion model and a transverse aortic constriction (TAC) were used adult WT Miat-null...
Cardiomyocytes (CMs), endothelial cells (ECs), smooth-muscle (SMCs), and cardiac fibroblasts (CFs) differentiated from human induced-pluripotent stem (hiPSCs) are the fundamental components of cell-based regenerative myocardial therapy can be used as in-vitro models for mechanistic studies drug testing. However, newly hiPSC-CMs tend to more closely resemble fetal CMs than mature adult hearts, current techniques improving CM maturation both complex labor-intensive. Thus, production commercial...
Human patatin-like phospholipase domain-containing 3 ( PNPLA 3) is associated with increased liver fat content and injury. Here, we show that nutritional status regulates PNPLA3 gene expression in the mouse liver. Sterol response element binding protein-1 (SREBP-1) activated transcription via sterol regulatory elements (SREs) mapped to promoter region. Chromatin immunoprecipitation electrophoretic mobility shift assays confirmed SREBP-1 proteins bound identified SREs. Furthermore, SREBP-1c...
Metabolic energy reprogramming facilitates adaptations to a variety of stress conditions and cellular dysfunction, but how the energetic demands are monitored met in response physiological stimuli remains elusive. Our data support model demonstrating that heat shock factor 1 (HSF1), master transcriptional regulator chaperone response, has been coopted from its role as critical protein quality-control having central systemic sensing for metabolic adaptation nutrient availability. We found...
Abstract Hepatic gluconeogenesis is essential for glucose homeostasis and also a therapeutic target type 2 diabetes, but its mechanism incompletely understood. Here, we report that Sam68, an RNA-binding adaptor protein Src kinase substrate, novel regulator of hepatic gluconeogenesis. Both global deletions Sam68 significantly reduce blood levels the glucagon-induced expression gluconeogenic genes. Protein, not mRNA, CRTC2, crucial transcriptional gluconeogenesis, are >50% lower in...
Diabetes, a chronic disease significantly affects peoples' health and quality of life. It is major contributor severe complications, such as blindness, kidney failure, heart attacks, strokes, lower limb amputations. Autophagy complex cellular process regulated by specific genes. responsible for degrading intracellular proteins organelles within lysosomes, which crucial maintaining internal balance clearing damaged or obsolete components. In the context diabetes, autophagy plays key role in...
MicroRNA-210 (miR-210) increases in hypoxia and regulates mitochondrial respiration through modulation of iron-sulfur cluster assembly proteins (ISCU1/2), a protein that is involved Fe/S synthesis. However, it not known how miR-210 affects cellular iron levels or production heme, another containing molecule also needed for function.To screen micro-ribonucleic acids (miRNAs) regulated by iron, we performed miRNA gene array neonatal rat cardiomyocytes treated with chelators. Levels are...
Abstract Toxoplasma gondii uracil phosphoribosyltransferase (UPRT) converts 4‐thiouracil (4TUc) into 4‐thiouridine (4TUd), which is incorporated nascent RNAs and can be biotinylated, then labelled with streptavidin conjugates or isolated via streptavidin‐affinity methods. Here, we generated mice that expressed T. UPRT only in cardiomyocytes ( CM mice) tested our hypothesis CM‐derived miRNAs miRs) are transferred remote organs after myocardial infarction (MI) by small extracellular vesicles...
Exosomes (Exo) are important mediators of inter-cellular communications; however, no effective method is available for isolating, thus characterizing, cellular-specific exosomes in vivo. Since CD63 a reliable marker exosomes, we have developed tagging strategy, term "CD63-Snorkel (CD63-SNKL)", which at its intracellular C-terminus was fused to fragment PDGFRB that contains the transmembrane domain tethered multiple epitope tags (HA, His, and FLAG) displayed tandem on surface. We found...
Recent studies have demonstrated that FoxO1 modulates the expression of SREBP-1c, but exact mechanism remains unknown. Our results demonstrate suppresses SREBP-1c promoter transcriptional activity in HepG2 cells. This repression was independent binding to promoter, LXR responsive elements (LXREs) were crucial this phenomenon. Moreover, also strongly inhibited LXRα-mediated elevated transcription by promoter. Electrophoretic mobility shift assay and chromatin immuno-precipitation further...
Rationale: Cell therapy for myocardial infarction is promising but largely unsuccessful in part due to a lack of mechanistic understanding. Techniques enabling identification stem cell-specific proteomes situ the injured heart may shed light on how administered cells respond microenvironment and exert reparative effects. Objective: To identify transplanted mesenchymal (MSCs) infarcted myocardium, we sought target mutant methionyl-tRNA synthetase (MetRSL274G) MSCs, which charges...
LncRNAs are involved in modulating the individual risk and severity of progression metabolic dysfunction-associated fatty liver disease (MASLD), but their precise roles remain largely unknown. This study aimed to investigate role lncRNA Snhg3 development MASLD, along with underlying mechanisms. The result showed that was significantly downregulated high-fat diet-induced obesity (DIO) mice. Notably, palmitic acid promoted expression overexpression increased lipid accumulation primary...
The orphan nuclear receptor SF-1 (steroidogenic factor 1) is highly expressed in the pituitary, gonad and adrenal glands plays key roles at all levels of hypothalamic–pituitary–steroidogenic tissue axis. In present study, we show that PGC-1α [PPARγ (peroxisome-proliferator-activated γ) co-activator 1α] interacts with co-activates to induce LHβ (luteinizing hormone β) αGSU (α-glycoprotein subunit) gene expression, subsequently leading increased secretion LH pituitary gonadotrope-derived αT3-1...
Activation of the adaptive response to cellular stress orchestrated by heat shock factor 1 (HSF1), which is an evolutionarily conserved transcriptional regulator chaperone and bioenergetics in diverse model systems, a central feature organismal defense from environmental stress. HSF1 activity, induced proteostatic, metabolic, growth signals, regulated posttranscriptional modifications, yet mechanisms that regulate particularly functional significance these modifications modulating its...
Using artificial insemination, 100 female quails were crossed with 10 male chickens. The eggs collected and hatched in the same incubator. sex of live hybrid embryos from 66 to 120 hatch hours was determined using multiply PCR Wpkci. Total 300 at various times sampled relative mRNA abundance ER, bcl-2, p53 detected by RT-PCR beta-actin as internal standard. effects on early embryonic development for hybrids between chicken quail analyzed. results showed ER expression higher than 84 a highly...
Abstract Background Angiostrongylus cantonensis L5, parasitizing human cerebrospinal fluid, causes eosinophilic meningitis, which is attributed to tissue inflammatory responses caused primarily by the high percentage of eosinophils. Eosinophils are also involved in killing helminths, using peroxidative oxidation and hydrogen peroxide (H 2 O ) generated dismutation superoxide produced during respiratory burst. In contrast, helminthic worms have evolved attenuate eosinophil-mediated for their...
Exosomes are a subtype of extracellular vesicles (EVs) with diameter 30~150 nm (averaging ~100 nm) that primarily produced through the endosomal pathway, and carry various components such as lipids, proteins, RNA, other small molecular substances. can mediate intercellular communication bioactive substances they carry, thus participating in different physiological activities. Metabolic syndrome (MS) is disease caused by disturbances body's metabolism, mainly including insulin resistance...