A5070277050- Extracellular vesicles in disease
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Silk-based biomaterials and applications
- Mitochondrial Function and Pathology
- Circular RNAs in diseases
- Tissue Engineering and Regenerative Medicine
- Neurobiology and Insect Physiology Research
- Cancer, Hypoxia, and Metabolism
- RNA modifications and cancer
- Antimicrobial Peptides and Activities
- Mesenchymal stem cell research
- Adipose Tissue and Metabolism
- Cardiac Fibrosis and Remodeling
- Insect Utilization and Effects
- Spectroscopy and Chemometric Analyses
- Metabolism, Diabetes, and Cancer
- Invertebrate Immune Response Mechanisms
- Cell Adhesion Molecules Research
- Anodic Oxide Films and Nanostructures
- Nanoplatforms for cancer theranostics
- Supramolecular Self-Assembly in Materials
- Biochemical and Structural Characterization
- RNA Research and Splicing
- Cardiovascular Function and Risk Factors
Southern University of Science and Technology
2023
University of Alabama at Birmingham
2020-2022
Soochow University
2016-2021
First Affiliated Hospital of Soochow University
2017-2021
State Key Laboratory of Silkworm Genomic Biology
2014-2017
Southwest University
2014-2017
Stem cell-derived exosomes have been recognized as a potential therapy for cardiovascular disease. However, the low retention rate of after transplantation in vivo remains major challenge clinical applications. The aim this study is to investigate whether human umbilical cord mesenchymal stem cell derived (UMSC-Exo) encapsulated functional peptide hydrogels could increase and stability improve heart function rat myocardial infarction model. Our results demonstrated that PA-GHRPS protected...
Vascular disease is a major complication of aging, but the molecular mechanisms underlying aging-induced vascular dysfunction remain unclear, and there no effective treatment to prevent aging induced diseases. The objectives present study are identify signaling pathway mediating develop an exosome based therapy inhibit process. We used 11-month-old C57BL6 mice as pre-aging animal model H2O2 treated H9C2 cells in vitro examine therapeutic effect miR-675. found decreased expression potential...
Background: Impaired energy metabolism in the heart is critical development of cardiac hypertrophy and failure. Src-associated mitosis 68 kDa (Sam68) a member signal transducer activator RNA (STAR) protein family, its role undefined. Methods: We assessed Sam68 expression human myopathic failing hearts. also generated mice with cardiomyocyte-specific deletion or overexpression subjected them to angiotensin II infusion transverse aortic constriction (TAC) surgery induce pathological...
Recent studies have shown that the hydrogels formed by composite biomaterials are better choice than single biomaterial for tissue repair. We explored feasibility of hydrogel silk fibroin (SF) and sericin (SS) in repair excellent mechanical properties SF, cell adhesion biocompatible SS. In our study, SF SS was protein with separate extraction method (LiBr dissolution hot alkaline water SS), while SF-SS using simultaneous protein). The effects two on release inflammatory cytokines from...
Abstract Toxoplasma gondii uracil phosphoribosyltransferase (UPRT) converts 4‐thiouracil (4TUc) into 4‐thiouridine (4TUd), which is incorporated nascent RNAs and can be biotinylated, then labelled with streptavidin conjugates or isolated via streptavidin‐affinity methods. Here, we generated mice that expressed T. UPRT only in cardiomyocytes ( CM mice) tested our hypothesis CM‐derived miRNAs miRs) are transferred remote organs after myocardial infarction (MI) by small extracellular vesicles...
Exosomes (Exo) are important mediators of inter-cellular communications; however, no effective method is available for isolating, thus characterizing, cellular-specific exosomes in vivo. Since CD63 a reliable marker exosomes, we have developed tagging strategy, term "CD63-Snorkel (CD63-SNKL)", which at its intracellular C-terminus was fused to fragment PDGFRB that contains the transmembrane domain tethered multiple epitope tags (HA, His, and FLAG) displayed tandem on surface. We found...
VgR, a member of the LDLR family, functions to transport vitellogenin into ovaries protome ovarian growth and embryonic development. In insects, only widely accepted ligand VgR is Vg. Recently, BmVgR has been shown interact with BmSP1 in vitro. Therefore, this study, we evaluated whether could certain cells. Although combine BmVg BmSP1, did not affect amount taken up by Sf9 Parallel immunofluorescence showed that most were localized inner oocyte membrane, showing tissue localization similar...
Exosomes transport biologically active cargo (e.g., proteins and microRNA) between cells, including many of the paracrine factors that mediate beneficial effects associated with stem-cell therapy. Stem cell derived exosomes, in particular mesenchymal stem cells (MSCs), have been shown previously to largely replicate therapeutic activity themselves, which suggests exosomes may be a useful cell-free alternative for treatment cardiovascular disorders. However, mechanisms govern how home damaged...
Genetic deletion of Src associated in mitosis 68kDa (Sam68), a pleiotropic adaptor protein prevents high-fat diet–induced weight gain and insulin resistance. To clarify the role Sam68 energy metabolism adult stage, we generated an inducible knockout mice. Knockout was induced at age 7-10 weeks, then examined metabolic profiles mice gained less body over time 34 or 36 weeks old, had smaller fat mass without changes food intake absorption efficiency. Deletion elevated thermogenesis, increased...
Exosomes are important mediators of inter-tissue communications; however, no effective method is available to allow for isolating, thus characterizing, tissue-specific exosomes in vivo. Since CD63 a reliable marker exosomes, we have developed tagging strategy, term “CD63-Snorkel (CD63-SNKL),” which at its intracellular C-terminus was fused fragment PDGFRB that contains the transmembrane domain tethered multiple epitope tags (HA, His, and FLAG) tandem on surface. We found CD63-SNKL protein...
Hepatic glucose production (HGP) is an important component of homeostasis, and deregulated HGP, particularly through gluconeogenesis, contributes to hyperglycemia pathology type-2 diabetes (T2D). It has been shown that the gluconeogenic gene expression governed primarily by transcription factor cAMP-response element (CRE)-binding protein (CREB) its coactivator, CREB-regulated transcriptional coactivator 2 (CRTC2). Recently, we have discovered Sam68, adaptor Src kinase substrate, potently...