A5101835072- Neurobiology and Insect Physiology Research
- Silk-based biomaterials and applications
- Insect Utilization and Effects
- Silkworms and Sericulture Research
- Insect and Arachnid Ecology and Behavior
- Invertebrate Immune Response Mechanisms
- Viral Infectious Diseases and Gene Expression in Insects
- Insect Resistance and Genetics
- Advanced Biosensing Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Insect and Pesticide Research
- Plant Virus Research Studies
- Biofuel production and bioconversion
- Aquaculture Nutrition and Growth
- CRISPR and Genetic Engineering
- Enzyme Catalysis and Immobilization
- Epigenetics and DNA Methylation
- RNA and protein synthesis mechanisms
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Entomopathogenic Microorganisms in Pest Control
- RNA Research and Splicing
- Chromosomal and Genetic Variations
- Animal Behavior and Reproduction
- Animal Genetics and Reproduction
Southwest University
2016-2025
South China University of Technology
2025
Ministry of Agriculture and Rural Affairs
2024
State Key Laboratory of Silkworm Genomic Biology
2013-2023
Sericultural Research Institute
2019-2023
Beijing Institute of Genomics
2004
BGI Group (China)
2004
Zhejiang University
2004
University of Washington
2004
We report a draft sequence for the genome of domesticated silkworm (Bombyx mori), covering 90.9% all known genes. Our estimated gene count is 18,510, which exceeds 13,379 genes reported Drosophila melanogaster. Comparative analyses to fruitfly, mosquito, spider, and butterfly reveal both similarities differences in content.
The Taming of the Silkworm Silkworms, Bombyx mori , represent one few domesticated insects, having been over 10,000 years ago. Xia et al. (p. 433 published online 27 August) sequenced 29 domestic and 11 wild silkworm lines identified genes that were most likely to be selected during domestication. These those enhance silk production, reproduction, growth. Furthermore, silkworms probably only once from a large progenitor population, rather than on multiple occasions, as has observed for other animals.
Establishment of efficient genome editing tools is essential for fundamental research, genetic engineering, and gene therapy. Successful construction application transcription activator-like effector nucleases (TALENs) in several organisms herald an exciting new era editing. We describe the production two active TALENs their successful targeted mutagenesis silkworm, Bombyx mori, whose manipulation methods are parallel to those Drosophila other insects. will also show that simultaneous...
Silkworms (Bombyx mori) produce massive amounts of silk proteins to make cocoons during the final stages larval development. Although major components, fibroin and sericin, have been focus for a long time, few researchers realized complexity proteome. We collected seven kinds fibers spun by silkworm larvae at different developmental stages: silks new hatched larvae, second instar day 0 third fourth molting scaffold used attach cocoon substrate silk. Analysis liquid chromatography-tandem mass...
A massively parallel array of monoclonal antibodies enables rapid antibody development and target discovery across species.
Vitellogenin receptors (VgRs) play crucial roles in oogenesis by mediating endocytosis of vitellogenin and other nutrients ovipara. We conducted small RNA sequencing screening with a luciferase reporter system, found that bmo-miR-2739 novel miRNA (novel-miR-167) coordinately regulate the expression VgR Bombyx mori (BmVgR). Further analyses suggested these two miRNAs direct target repression binding directly to BmVgR 3' untranslated region. Forced either using piggyBac system blocked (Vg)...
<title>Abstract</title> The dynamic cellular transitions, spatial organization, and hormonal regulatory mechanisms of insect wing development are incompletely understood. Here, we reported an integrated spatially resolved single-cell atlas the silkworm (Bombyx mori) disc across 10 developmental time points, investigating dynamics 12 cell types. Wing morphogenesis (Wm) cells were characterized as central progenitors with bifurcated differentiation toward epithelial cuticle lineages, regulated...
Abstract Nutrient accumulation is essential for insect metamorphosis. As a group of important nutrient‐storage proteins, forty‐six 30 K proteins (30KPs), including BmLP1‐BmLP46, have been identified in the silkworm, Bombyx mori . Most 30KPs are synthesised last instar larvae, and stage‐specific expression believed to be regulated by juvenile hormone (JH)‐dependent pathways; however, specific regulatory mechanism remains unclear. In this study, we found that 30KP gene Bmlp1 was expressed...
Estrogen-related receptors (ERRs) play indispensable roles in development, energy metabolism, and cancers are metabolic switches Drosophila. However, the mechanism underlying their role is unknown insects. This study analysed expression profiles of Bombyx mori ERR (BmERR), hexokinase (BmHK), pyruvate kinase (BmPK) phosphofructokinase (BmPFK) during embryonic development. The BmERR tended to be similar that other genes. We observed a regulatory association between glycolytic rate-limiting...
Abstract A group of lipoproteins with molecular sizes approximately 30 kDa, referred to as 30K proteins, are synthesized in fat body cells the fifth instar larvae silkworm, Bombyx mori. Analyzing silkworm genome and its expressed sequence tags (ESTs), we found 10 genes encoding which mainly distributed three subfamilies. Of these, seven coding proteins were harbor degrading sites 30kP protease A, although number may be different. As some potential core promoters regulatory elements supposed...
Abstract Vitellogenin (Vg) is a source of nutrition for embryo development. Our previous study showed that the silkworm ( Bombyx mori ) transcription factor broad complex isoform 2 (BmBrC‐Z2) regulates gene expression Vg BmVg by induction with 20‐hydroxyecdysone (20E). However, mechanism which 20E was not clarified. In this study, cell transfection experiments promoter containing POU homeodomain POUM2 (POUM2) and BrC‐Z2 cis ‐response elements (CREs) more significant response to than...
Abstract Oestrogen‐related receptor (ERR) is involved in oestrogen (ER) signalling pathways owing to its similarity ER terms of domain structure and co‐activator response elements. Although insects lack ER, they harbour an ERR gene that thought modulate metabolism energy conversion via unknown mechanism. The present study investigated the function using female silkworm ( Bombyx mori , Bm). We found expression B. vitellogenin (BmVg) (BmERR) fat bodies silkworms at different stages development...
Estrogen-related receptor (ERR) is an orphan nuclear that was first discovered in animals, and play important role metabolism, development, reproduction. Despite extensive research on the function of ERR, its transcriptional regulation mechanism remains unclear. In this study, we obtained upstream region Bombyx mori ERR (BmERR) confirmed promoter activity region. Interestingly, found 10 50 nM 20-hydroxyecdysone (20E) up-regulated BmERR promoter. addition, eight putative ecdysone response...
The silk of silkworm consists fibroin fiber coated by sericins. In addition, some nonprotein components were also identified in the sericin fraction. presence has not been well explained. present study, methods based on gas chromatography–mass spectrometry used to identify metabolites cocoon from a wild and two domestic strains. total, 45 silk, including organic acids, fatty carbohydrates, amino hydrocarbons. Comparative analyses revealed that 17 significant more than cocoon, three aldoses,...
VgR, a member of the LDLR family, functions to transport vitellogenin into ovaries protome ovarian growth and embryonic development. In insects, only widely accepted ligand VgR is Vg. Recently, BmVgR has been shown interact with BmSP1 in vitro. Therefore, this study, we evaluated whether could certain cells. Although combine BmVg BmSP1, did not affect amount taken up by Sf9 Parallel immunofluorescence showed that most were localized inner oocyte membrane, showing tissue localization similar...