A5081835140- Plant Molecular Biology Research
- Plant Reproductive Biology
- Photosynthetic Processes and Mechanisms
- CRISPR and Genetic Engineering
- Plant-Microbe Interactions and Immunity
- Plant Virus Research Studies
- Transgenic Plants and Applications
- Chromosomal and Genetic Variations
- Lipid metabolism and biosynthesis
- Plant Disease Resistance and Genetics
- Molecular Biology Techniques and Applications
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- Plant nutrient uptake and metabolism
- Nuclear Structure and Function
- Genetics and Neurodevelopmental Disorders
- Light effects on plants
- Legume Nitrogen Fixing Symbiosis
- Genomics and Phylogenetic Studies
- Plant tissue culture and regeneration
Texas State University
2014-2025
Thermo Fisher Scientific (United States)
2021-2025
The University of Texas at Austin
2020-2023
Changes in ambient temperature immensely affect developmental programs many species. Plants adapt to high growth part by vegetative and reproductive reprogramming, known as thermo-morphogenesis. Thermo-morphogenesis is accompanied massive changes the transcriptome upon change. Here, we show that induced warm require VERNALIZATION INSENSITIVE 3-LIKE 1 (VIL1), a facultative component of Polycomb repressive complex PRC2, Arabidopsis. Warm elicits genome-wide accumulation H3K27me3 VIL1 necessary...
Polycomb dictates developmental programs in higher eukaryotes, including flowering plants. A phytohormone, abscisic acid (ABA), plays a pivotal role seed and seedling development mediates responses to multiple environmental stresses, such as salinity drought. In this study, we show that ABA affects the Repressive Complex 2 (PRC2)-mediated Histone H3 Lys 27 trimethylation (H3K27me3) through VIN3-LIKE1/VERNALIZATION 5 (VIL1/VRN5) fine-tune timely repression of ABSCISIC ACID INSENSITIVE 3...
The construction and application of synthetic genetic circuits is frequently improved if gene expression can be orthogonally controlled, relative to the host. In plants, orthogonality achieved via use CRISPR-based transcription factors that are programmed act on natural or promoters. complex require multiple, orthogonal regulatory interactions, this in turn requires full programmability CRISPR elements adapted non-natural non-standard promoters have few constraints their design. Therefore,...
To assess the role of MORC1 in epigenetics relation to plant immunity, genome-wide chromatin accessibility was compared between mock- or Pseudomonas syringae pv. tomato–inoculated wild type (WT) Arabidopsis, morc1/2 double mutant, both. Most changes accessibility, scored by DNase I hypersensitive sites (DHSs), were located promoters genes and transposable elements (TEs). Comparisons WT receiving same treatment revealed differential DHSs (dDHSs) predominantly associated with heterochromatic...
MORC1 and MORC2, two of the seven members Arabidopsis (Arabidopsis thaliana) Compromised Recognition Turnip Crinkle Virus1 subfamily microrchidia Gyrase, Heat Shock Protein90, Histidine Kinase, MutL (GHKL) ATPases, were previously shown to be required in multiple layers plant immunity. Here, we show that barley (Hordeum vulgare) MORCs also are involved disease resistance. Genome-wide analyses identified five 37% 48% identical on protein level AtMORC1. Unexpectedly, clear contrast...
Summary LIKE HETEROCHROMATIN PROTEIN1 (LHP1) encodes the only plant homologue of metazoan (HP1) protein family. The LHP1 is necessary for proper epigenetic regulation a range developmental processes in plants. transcriptional repressor flowering‐related genes, such as FLOWERING LOCUS T ( FT ), C FLC AGAMOUS AG ) and APETALA 3 AP3 ). We found that interacts with importin α‐1 (IMPα‐1), α‐2 (IMPα‐2) α‐3 (IMPα‐3) both vitro vivo . A genetic approach revealed triple mutation impα‐1 , impα‐2...
Evolutionarily conserved DEK domain-containing proteins have been implicated in multiple chromatin-related processes, mRNA splicing and transcriptional regulation eukaryotes. Here, we show that two proteins, DEK3 DEK4, control the floral transition Arabidopsis. DEK4 directly associate with chromatin of related flowering repressors, FLOWERING LOCUS C (FLC), its homologs, MADS AFFECTING FLOWERING4 (MAF4) MAF5, to promote their expression. The binding a histone octamer vivo affects...
Abstract Adapting to the everchanging environment is key a successful life for an organism. Eukaryotes reprogram their transcriptome in order adapt unfavorable environment. To achieve this reprogramming, plants and animals employ multiple responses including epigenetic regulation. In search mutations compromised high ambient temperature response, we found that VIL1, PHD finger protein displays aberrant development at temperature. RNA-seq analysis shows vil1 fails downregulate heat suppressed...
Abstract Background: The construction and application of synthetic genetic circuits is frequently improved if gene expression can be orthogonally controlled, relative to the host. In plants, orthogonality achieved via use CRISPR-based transcription factors that are programmed act on natural or promoters. complex require multiple, orthogonal regulatory interactions, this in turn requires full programmability CRISPR elements adapted non-natural non-standard promoters have few constraints their...
Abstract Background The construction and application of synthetic genetic circuits is frequently improved if gene expression can be orthogonally controlled, relative to the host. In plants, orthogonality achieved via use CRISPR-based transcription factors that are programmed act on natural or promoters. complex require multiple, orthogonal regulatory interactions, this in turn requires full programmability CRISPR elements adapted non-natural non-standard promoters have few constraints their...