A5100349752- Plant Molecular Biology Research
- Pesticide Residue Analysis and Safety
- Aquaculture Nutrition and Growth
- Light effects on plants
- Genomics and Phylogenetic Studies
- Photosynthetic Processes and Mechanisms
- Chromosomal and Genetic Variations
- Plant and Fungal Species Descriptions
- CRISPR and Genetic Engineering
- Ecology and Conservation Studies
- Insect and Pesticide Research
- Plant Virus Research Studies
- Food Quality and Safety Studies
- Pharmacological Effects and Assays
- Plant Stress Responses and Tolerance
- Plant Genetic and Mutation Studies
- Agriculture, Soil, Plant Science
- Fish Biology and Ecology Studies
- RNA and protein synthesis mechanisms
- Advancements in Solid Oxide Fuel Cells
- Mycotoxins in Agriculture and Food
- Antibiotics Pharmacokinetics and Efficacy
- Natural product bioactivities and synthesis
- Reproductive biology and impacts on aquatic species
- Plant-Microbe Interactions and Immunity
National Cancer Institute
2004-2024
The University of Texas at Austin
2011-2024
Korea Photonics Technology Institute
2024
Seoul Institute
2022
National Institute of Biological Resources
2014-2021
National Institute of Fisheries Science
2018-2020
Chung-Ang University
2011-2018
Myongji University
2017
Korea University
2017
Sejong University
2017
Abstract Seeds monitor the environment to germinate at proper time, but different species respond differently environmental conditions, particularly light and temperature. In Arabidopsis thaliana, promotes germination high temperature suppresses germination. We previously reported that by repressing SOMNUS (SOM). Here, we examined whether also regulates through SOM found activates expression. Consistent with this, som mutants germinated more frequently than wild type The induction of mRNA...
Phytochromes are red and far-red light receptors in plants that mediate critical responses to throughout the lifecycle. They achieve this part by targeting negatively acting bHLH transcription factors called phytochrome-interacting (PIFs) for degradation within nucleus. However, it is not known whether protein primary mechanism which phytochromes inhibit these repressors of photomorphogenesis. Here, we use chromatin immunoprecipitation show phyB inhibits regulatory activity PIF1 PIF3...
Light is a crucial environmental factor that impacts various aspects of plant development. Phytochromes, as light sensors, regulate myriads downstream genes to mediate developmental reprogramming in response changes conditions. CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1) an E3 ligase for number substrates signaling, acting central repressor photomorphogenesis. The interplay between phytochrome B (phyB) and COP1 forms antagonistic regulatory module triggers extensive gene expression when exposed...
The bHLH transcription factor PHYTOCHROME INTERACTING FACTOR1 (PIF1) binds G-box elements in vitro and inhibits light-dependent germination Arabidopsis thaliana. A previous genome-wide analysis of PIF1 targeting indicated that 748 sites imbibed seeds, only 59% which possess elements. This suggests the is not sole determinant targeting. to specific could be stabilized by PIF1-interacting factors (PTFs) bind other nearby sequence Here, we report are enriched with G-boxes but also hexameric...
Despite the key role of human ribosome in protein biosynthesis, little is known about extent sequence variation ribosomal DNA (rDNA) or its pre-rRNA and rRNA products. We recovered segments from a single chromosome 21 using transformation-associated recombination (TAR) cloning yeast. Accurate long-read sequencing 13 isolates covering ∼0.82 Mb rDNA complement revealed substantial among tandem repeat copies, several palindromic structures potential errors previous reference sequence. These...
Elongated hypocotyl5 (HY5) is a key transcription factor that promotes photomorphogenesis. Constitutive photomorphogenic1 (COP1)-Suppressor of phytochrome A-105 (SPA) E3 ubiquitin ligase complex ubiquitination and degradation HY5 to repress photomorphogenesis in darkness. also regulated by phosphorylation at serine 36 residue. However, the kinase responsible for remains unknown. Here, using extensive vitro vivo biochemical, genetic, photobiological techniques, we have identified new...
Human artificial chromosome (HAC)-based vectors offer a promising system for delivery and expression of full-length human genes any size. HACs avoid the limited cloning capacity, lack copy number control, insertional mutagenesis caused by integration into host chromosomes that plague viral vectors. We previously described synthetic HAC can be easily eliminated from cell populations inactivation its conditional kinetochore. Here, we demonstrate utility this HAC, which has unique gene acceptor...
Changes in ambient temperature immensely affect developmental programs many species. Plants adapt to high growth part by vegetative and reproductive reprogramming, known as thermo-morphogenesis. Thermo-morphogenesis is accompanied massive changes the transcriptome upon change. Here, we show that induced warm require VERNALIZATION INSENSITIVE 3-LIKE 1 (VIL1), a facultative component of Polycomb repressive complex PRC2, Arabidopsis. Warm elicits genome-wide accumulation H3K27me3 VIL1 necessary...
In human chromosomes, centromeric regions comprise megabase-size arrays of 171 bp alpha-satellite DNA monomers.The large distances spanned by these preclude their replication from external sites and imply that the repetitive monomers contain origins.However, within has not previously been profiled role in initiation yet demonstrated.Here, endogenous de novo formed Human Artificial Chromosome (HAC) was analyzed.We showed could function as origins alphoid organized into centrochromatin...
This study aimed to examine the microbial contamination levels in livestock products at retail stores. Beef, pork, and chicken samples from raw materials final were obtained between January December 2015. All homogenized meat (25 g) tested for aerobic plate count (APC), coliform (CC), Escherichia coli (E. coli). The highest APCs samples, by month, shops September, followed July, May, October (p<0.001). However, APC was summer lowest winter Average beef, 2.90, 3.19, 3.79 Log CFU/g,...
Polycomb dictates developmental programs in higher eukaryotes, including flowering plants. A phytohormone, abscisic acid (ABA), plays a pivotal role seed and seedling development mediates responses to multiple environmental stresses, such as salinity drought. In this study, we show that ABA affects the Repressive Complex 2 (PRC2)-mediated Histone H3 Lys 27 trimethylation (H3K27me3) through VIN3-LIKE1/VERNALIZATION 5 (VIL1/VRN5) fine-tune timely repression of ABSCISIC ACID INSENSITIVE 3...
The construction and application of synthetic genetic circuits is frequently improved if gene expression can be orthogonally controlled, relative to the host. In plants, orthogonality achieved via use CRISPR-based transcription factors that are programmed act on natural or promoters. complex require multiple, orthogonal regulatory interactions, this in turn requires full programmability CRISPR elements adapted non-natural non-standard promoters have few constraints their design. Therefore,...
The reported human genome sequence includes about 400 gaps of unknown that were not found in the bacterial artificial chromosome (BAC) and cosmid libraries used for sequencing genome. These missing sequences correspond to ∼1% euchromatic regions Gap filling is a laborious process because it relies on analysis random clones numerous genomic BAC or libraries. In this work we demonstrate closing can be accelerated by selective recombinational capture chromosomal segments yeast. use both...
Drought stress conditions in soil or air hinder plant growth and development. Here, we report that the hot pepper (C apsicum a nnuum) RING type E3 Ligase 1 gene (CaREL1) is essential to drought response. CaREL1 encodes cytoplasmic- nuclear-localized protein with ligase activity. expression was induced by abscisic acid (ABA) drought. contains C3H2C3-type finger motif, which functions ubiquitination of target protein. We used CaREL1-silenced plants CaREL1-overexpressing (OX) transgenic...
Chromatin architectural regulators antagonize gene looping to control flowering time in Arabidopsis .
A simple and rapid method for the simultaneous determination of 11 mycotoxins - aflatoxins B1, B2, G1 G2; fumonisins B2 B3; ochratoxin A; zearalenone; deoxynivalenol; T-2 toxin in edible oils was established using liquid chromatography tandem mass spectrometry (LC-MS/MS). In this study, QuEChERS (Quick, Easy, Cheap, Effective, Rugged Safe), with dispersive liquid-liquid microextraction, solvent extraction were examined sample preparation. Among these methods, a mixture formic...
A multi-class, multi-residue analytical method based on LC-MS/MS detection was developed for the screening and confirmation of 28 veterinary drug metabolite residues in flatfish, shrimp eel. The chosen drugs are prohibited or unauthorised compounds Korea, which were categorised into various chemical classes including nitroimidazoles, benzimidazoles, sulfones, quinolones, macrolides, phenothiazines, pyrethroids others. To achieve fast simultaneous extraction analytes, a simple generic liquid...
Conventional flower or leaf recognition studies have some restrictions and limitations. These include a sharp drop in rate due to the varying positions number of relevant objects original object image. Hence, this paper suggests implements mobile-based system using Difference Image Entropy (DIE) contour features from image with multi-flower objects. In framework includes 1) WiBro Net.-based transmission designation module by drawing region user's interest, 2) feature extraction module, 3)...
Abstract Ribosomal DNA (rDNA) repeat units are organized into tandem clusters in eukaryotic cells. In mice, these located on at least eight chromosomes and show extensive variation the number of repeats between mouse genomes. To analyze intra- inter-genomic rDNA repeats, we selectively isolated 25 individual using Transformation-Associated Recombination (TAR) cloning. Long-read sequencing subsequent comparative sequence analysis revealed that each full-length unit comprises an intergenic...