A5056878388- RNA and protein synthesis mechanisms
- Advanced MRI Techniques and Applications
- RNA modifications and cancer
- Functional Brain Connectivity Studies
- Ubiquitin and proteasome pathways
- DNA and Nucleic Acid Chemistry
- Antimicrobial Peptides and Activities
- NMR spectroscopy and applications
- Chemical Synthesis and Analysis
- Enzyme Structure and Function
- Advanced NMR Techniques and Applications
- Biochemical and Structural Characterization
- Protein Structure and Dynamics
- Bacterial Genetics and Biotechnology
- Neuroscience and Neuropharmacology Research
- RNA Research and Splicing
- Advanced Neuroimaging Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Glycosylation and Glycoproteins Research
- Cancer-related Molecular Pathways
- Carbohydrate Chemistry and Synthesis
- Genomics and Chromatin Dynamics
- Microtubule and mitosis dynamics
- Cancer therapeutics and mechanisms
- Viral Infections and Immunology Research
United States Department of State
2025
Korea Basic Science Institute
2014-2023
Korea University of Science and Technology
2008-2022
Cheongju University
2022
Seoul St. Mary's Hospital
2012
Catholic University of Korea
2012
University of Science and Technology
2012
Bioanalytica (Switzerland)
2012
Icahn School of Medicine at Mount Sinai
2012
Athinoula A. Martinos Center for Biomedical Imaging
2012
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStructure of an unusually stable RNA hairpinGabriele Varani, Chaejoon Cheong, and Ignacio Tinoco, Jr.Cite this: Biochemistry 1991, 30, 13, 3280–3289Publication Date (Print):April 2, 1991Publication History Published online1 May 2002Published inissue 2 April 1991https://pubs.acs.org/doi/10.1021/bi00227a016https://doi.org/10.1021/bi00227a016research-articleACS PublicationsRequest reuse permissionsArticle Views737Altmetric-Citations206LEARN ABOUT...
The relation between DNA polymerase fidelity and base pairing stability is investigated by using primer-template duplexes that contain a common 9-base template sequence but have either correct (A.T) or incorrect (G.T, C.T, T.T) pairs at the primer 3' terminus. Thermal melting enzyme kinetic measurements are compared for each kind of Analysis temperatures finds differences free energy changes upon dissociation (delta delta Go) only 0.2, 0.3, 0.4 kcal.mol-1 (1 cal = 4.18 J) terminal A.T to...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSolution structure of an unusually stable RNA tetraplex containing G- and U-quartet structuresChaejoon Cheong Peter B. MooreCite this: Biochemistry 1992, 31, 36, 8406–8414Publication Date (Print):September 15, 1992Publication History Published online1 May 2002Published inissue 15 September 1992https://pubs.acs.org/doi/10.1021/bi00151a003https://doi.org/10.1021/bi00151a003research-articleACS PublicationsRequest reuse permissionsArticle...
The outer membrane protein A (OmpA) plays important roles in anchoring of the to bacterial cell wall. C-terminal periplasmic domain OmpA (OmpA-like domain) associates with peptidoglycan (PGN) layer noncovalently. However, there is a paucity information on structural aspects mechanism PGN recognition by OmpA-like domains. To elucidate this molecular process, we solved high-resolution crystal structure an from Acinetobacter baumannii bound diaminopimelate (DAP), unique amino acid PGN. clearly...
In eukaryotic cells, apoptosis and cell cycle arrest by the Ras --> RASSF MST pathway are controlled interaction of SARAH (for Salvador/Rassf/Hippo) domains in C-terminal part tumor suppressor proteins. The Mst1 domain interacts with its homologous Rassf1 Rassf5 (also known as Nore1) forming a heterodimer that mediates process. Here, we describe homodimeric structure human heterotypic Salvador (Sav) domain. forms homodimer containing two helices per monomer. An antiparallel arrangement long...
To understand the fates of human mesenchymal stem cells (hMSCs) following transplantation into a rodent model middle cerebral artery occlusion (MCAo), magnetic resonance imaging (MRI) techniques were employed, hMSCs labeled with ferumoxides (Feridex)--protamine sulfate complexes, which visualized and examined by MRI up to 10 weeks transplantation. Migration transplanted infarcted area was further confirmed histological methods. We found that in MCAo models possess capacity migrate...
RATIONALE Alkaloids with significant therapeutic effects are the main active constituents of Corydalis ( C .) species. There several kinds alkaloids in . species associated diverse alkaloid metabolism plants, but they rarely identified. This study aimed to identify by liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI‐MS/MS). METHODS Several types were extracted from using ultrasonication 70% CH 3 OH, and extract was partitioned at pH 2 12. Separation achieved C18...
Trauma, mental illnesses, and substance use disorders (SUD) are well-documented contributors leading to women’s incarceration; however, less is known about how these factors also influence pathways through prison once incarcerated. To address this gap, we examined (1) prison-based health services, (2) summarized their sociodemographic diagnostic profiles, (3) addiction relate indicators of within-prison functioning, intervention receipt, recidivism. Data derived from routine administrative...
The influenza A virus, a severe pandemic pathogen, has segmented RNA genome consisting of eight single-stranded molecules. 5' and 3' ends each segment recognized by the virus RNA-dependent polymerase direct both transcription replication virus's genome. Promoter binding viral formation an active open complex are prerequisites for proliferation. Here we describe solution structure this promoter as solved multidimensional, heteronuclear magnetic resonance spectroscopy. Our studies show that...
The thermal stabilities of oligodeoxyribonucleotide duplexes containing 2,6-diaminopurine (D) matched with each the four normal DNA bases were determined by optical melting techniques. Comparison curves yielded relative for D-containing standard base pairs in an otherwise identical base-pair sequence. D:T pair was found to be more stable than A:T dC3DG3:dC3TG3, as dCT3DT3G:dCA3TA3G, and less dCA3DA3G:dCT7G. order X:Y duplex dCA3XA3G:dCT3YT3G is: (A:T) greater (T:D) congruent (D:T) or equal...
Heparin lyase I (heparinase I) specifically depolymerizes heparin, cleaving the glycosidic linkage next to iduronic acid. Here, we show crystal structures of heparinase from Bacteroides thetaiotaomicron at various stages reaction with heparin oligosaccharides before and just after cleavage product disaccharide. The structure is comprised a beta-jellyroll domain harboring long deep substrate binding groove an unusual thumb-resembling extension. This thumb, decorated many basic residues,...
E2-25K/Hip2 is an unusual ubiquitin-conjugating enzyme that interacts with the frameshift mutant of ubiquitin B (UBB(+1)) and has been identified as a crucial factor regulating amyloid-β neurotoxicity. To study structural basis neurotoxicity mediated by E2-25K-UBB(+1) interaction, we determined three-dimensional structures UBB(+1), E2-25K E2-25K/ubiquitin, E2-25K/UBB(+1) complex. The revealed or UBB(+1) bound to via MGF motif residues in α9 enzyme. Polyubiquitylation assays together analyses...
Background— Limited availability of noninvasive and biologically precise diagnostic tools poses a challenge for the evaluation management patients with myocarditis. Methods Results— The feasibility cardiovascular magnetic resonance (CMR) imaging magneto-fluorescent nanoparticles (MNPs) detection myocarditis its effectiveness in discriminating inflammation grades were assessed experimental autoimmune (EAM) (n=65) control (n=10) rats. After undergoing CMR, rats administered MNPs, followed by...
Despite recent progress in research on the Hippo signalling pathway, structural information available this area is extremely limited. Intriguingly, homodimeric and heterodimeric interactions of mammalian sterile 20-like (MST) kinases through so-called `SARAH' (SAV/RASSF/HPO) domains play a critical role cellular homeostasis, dictating fate cell regarding proliferation or apoptosis. To understand mechanism heterodimerization SARAH domains, three-dimensional structures an MST1–RASSF5...
Three new compounds, a diarylheptanone glucoside (1), 4,5,8-trihydroxy-α-tetralone 5-O-β-d-[6'-O−(3'',5''-dimethoxy-4''-hydroxybenzoyl)]glucopyranoside (2), and 1,4,8-trihydroxy-3-naphthalenecarboxylic acid 1-O-β-d-glucopyranoside methyl ester (3), were isolated from the roots of Juglans mandshurica, their structures elucidated on basis spectroscopic studies including 2D-NMR.
The structure of 21-residue antimicrobial peptide buforin II has been determined by using NMR spectroscopy and restrained molecular dynamics. Buforin adopts a flexible random in H2O. In trifluoroethanol (TFE)/H2O (1:1, v/v) mixture, however, assumes regular alpha-helix between residues Val12 Arg20 distorted helical Gly7 Pro11. model obtained shows an amphipathic character the region from Arg5 to C-terminus, Lys21. Like other known cationic peptides, might be key factor for activity II.
Abstract CpG oligodeoxynucleotides (ODNs) are promising immunomodulatory agents for treating human diseases and vaccine development. Phosphodiester ODNs were demonstrated to have poor immunostimulatory potentials cytokine production. However, the conjugation of consecutive deoxyriboguanosine residues, called a dG run, at 3′ terminus phosphodiester significantly enhanced TNF-α IL-12 production from mouse splenic dendritic cells (DCs). The optimal induction was achieved by addition hexameric...
Two-component signal transduction systems, commonly found in prokaryotes, typically regulate cellular functions response to environmental conditions through a phosphorylation-dependent process. A new type of regulator, hp1043 (HP-RR) from Helicobacter pylori, has been recently identified. HP-RR is essential for cell growth and does not require the well known phosphorelay scheme. Unphosphorylated binds specifically its own promoter (P(1043)) autoregulates tlpB gene (P(tlpB)). We have...