A5042988495- Enzyme Structure and Function
- Protein Structure and Dynamics
- Glycosylation and Glycoproteins Research
- RNA and protein synthesis mechanisms
- Proteoglycans and glycosaminoglycans research
- Biochemical Analysis and Sensing Techniques
- Protein Kinase Regulation and GTPase Signaling
- Cell Adhesion Molecules Research
- Mass Spectrometry Techniques and Applications
- Regulation of Appetite and Obesity
- Monoclonal and Polyclonal Antibodies Research
- Ubiquitin and proteasome pathways
- Protease and Inhibitor Mechanisms
- RNA modifications and cancer
- Telomeres, Telomerase, and Senescence
- Hippo pathway signaling and YAP/TAZ
- Molecular spectroscopy and chirality
- Cancer-related gene regulation
- Peptidase Inhibition and Analysis
- Melanoma and MAPK Pathways
- Computational Drug Discovery Methods
- Wnt/β-catenin signaling in development and cancer
- Receptor Mechanisms and Signaling
- Cytokine Signaling Pathways and Interactions
- Plasma Applications and Diagnostics
Yonsei University
2014-2024
University of Wisconsin–Milwaukee
2019
University of Tsukuba
2009
Institute of Applied Biochemistry
2009
Korea University
2008
Korea Basic Science Institute
2008
Ontario Institute for Cancer Research
1994-2005
University of Toronto
1994-2005
Yonsei Proteome Research Center
2005
East Tennessee State University
2002
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA Suite of Triple Resonance NMR Experiments for the Backbone Assignment 15N, 13C, 2H Labeled Proteins with High SensitivityToshio Yamazaki, Weontae Lee, Cheryl H. Arrowsmith, D. R. Muhandiram, and Lewis E. KayCite this: J. Am. Chem. Soc. 1994, 116, 26, 11655–11666Publication Date (Print):December 1, 1994Publication History Published online1 May 2002Published inissue 1 December...
Around 250 million people are infected with hepatitis B virus (HBV) worldwide1, and 15 may also carry the satellite D (HDV), which confers even greater risk of severe liver disease2. The HBV receptor has been identified as sodium taurocholate co-transporting polypeptide (NTCP), interacts directly first 48 amino acid residues N-myristoylated N-terminal preS1 domain viral large protein3. Despite pressing need for therapeutic agents to counter HBV, structure NTCP remains unsolved. This...
The influx of genomic sequence information has led to the concept structural proteomics, determination protein structures on a genome-wide scale. Here we describe an approach proteomics small proteins using NMR spectroscopy. Over 500 from several organisms were cloned, expressed, purified, and evaluated by NMR. Although there was variability among proteomes, overall 20% these found be readily amenable structure determination. sample preparation centralized in one facility, distributive used...
Plasma medicine is an upcoming research area that has attracted the scientists to explore more deeply utility of plasma. So, apart from treating biomaterials and tissues with plasma, we have studied effect soft plasma different feeding gases such as Air, N2 Ar on modification biomolecules. Hence, in this work used biomolecules proteins ((Hemoglobin (Hb) Myoglobin (Mb)), calf thymus DNA amino acids. The structural changes or been using circular dichroism (CD), fluorescence spectroscopy,...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAn HNCA Pulse Scheme for the Backbone Assignment of 15N,13C,2H-Labeled Proteins: Application to a 37-kDa Trp Repressor-DNA ComplexToshio Yamazaki, Weontae Lee, Matthew Revington, Debra L. Mattiello, Frederick W. Dahlquist, Cheryl H. Arrowsmith, and Lewis E. KayCite this: J. Am. Chem. Soc. 1994, 116, 14, 6464–6465Publication Date (Print):July 1, 1994Publication History Published online1 May 2002Published inissue 1 July...
Abstract The variation in the biological function of proteins plays an important role plasma medicine and sterilization. Several non-thermal sources with different feeding gases are used worldwide for treatment, including dielectric barrier discharge (DBD) atmospheric-pressure jet (APPJ) as most commonly sources. Therefore, present work, we both DBD APPJ N 2 air to evaluate effects on structural, thermodynamic, activity changes enzymes. In current lysozyme a model enzyme verified structural...
The phosphoinositide 3-kinase-Akt signaling pathway is essential to many biological processes, including cell proliferation, survival, metabolism, and angiogenesis, under pathophysiological conditions. Although 3-phosphoinositide-dependent kinase 1 (PDK1) a primary activator of Akt at the plasma membrane, optimal activation mechanism remains unclear. We report that adhesion molecule with IgG-like domain 2 (AMIGO2) novel scaffold protein regulates PDK1 membrane localization activation. Loss...
Abstract A novel light-driven chloride-pumping rhodopsin (ClR) containing an ‘NTQ motif’ in its putative ion conduction pathway has been discovered and functionally characterized a genomic analysis study of marine bacterium. Here we report the crystal structure ClR from flavobacterium Nonlabens marinus S1-08 T determined under two conditions at 2.0 1.56 Å resolutions. The structures reveal chloride-binding sites, one around protonated Schiff base other on cytoplasmic loop. We identify ‘3...
Significance Light-driven rhodopsin proteins pump ions across cell membranes. They have applications in optogenetics and can potentially be used to develop solar energy–harvesting devices. A detailed understanding of dynamics functions may therefore assist research medicine, health, clean energy. This time-resolved crystallography study carried out with X-ray free-electron lasers reveals chloride ion–pumping (ClR) within 100 ps light activation. It shows the dissociation Cl − from Schiff...
Syndecan-4, a transmembrane heparan sulfate proteoglycan, is coreceptor with integrins in cell adhesion. It has been suggested to form ternary signaling complex protein kinase Cα and phosphatidylinositol 4,5-bisphosphate (PIP<sub>2</sub>). Syndecans each have unique, central, variable (V) region their cytoplasmic domains, that of syndecan-4 critical its interaction C PIP<sub>2</sub>. Two oligopeptides corresponding the (4V) whole domain (4L) were synthesized for nuclear magnetic resonance...
E2-25K/Hip2 is an unusual ubiquitin-conjugating enzyme that interacts with the frameshift mutant of ubiquitin B (UBB(+1)) and has been identified as a crucial factor regulating amyloid-β neurotoxicity. To study structural basis neurotoxicity mediated by E2-25K-UBB(+1) interaction, we determined three-dimensional structures UBB(+1), E2-25K E2-25K/ubiquitin, E2-25K/UBB(+1) complex. The revealed or UBB(+1) bound to via MGF motif residues in α9 enzyme. Polyubiquitylation assays together analyses...
Research Article3 March 2016Open Access Transparent process Small molecule inhibitors of the Dishevelled-CXXC5 interaction are new drug candidates for bone anabolic osteoporosis therapy Hyun-Yi Kim Translational Center Protein Function Control, Yonsei University, Seoul, Korea Department Biotechnology, College Life Science and Search more papers by this author Sehee Choi Ji-Hye Yoon Biochemistry, Hwan Jung Lim Institute Chemical Technology, Daejeon, Hyuk Lee Jiwon Bioinformatics & Molecular...
Δ5-3-Ketosteroid isomerase from Pseudomonas testosteroni has been intensively studied as a prototype to understand an enzyme-catalyzed allylic isomerization. Asp38 (pK ∼4.7) was identified the general base abstracting steroid C4β proton ∼12.7) form dienolate intermediate. A key and common enigmatic issue involved in abstraction is question of how energy required for unfavorable transfer can be provided at active site enzyme and/or thermodynamic barrier drastically reduced. Answering this...
It is shown that the chiral selector (+)-(18-crown-6)-2,3,11,12-tetracarboxylic acid (18-C-6-TA) employed for resolution of α-amino acids in capillary electrophoresis and HPLC can be used ester derivatives NMR experiments. In a quest origin recognition presence 18-C-6-TA as selector, these interactions responsible differential affinities toward enantiomers are investigated by spectroscopy. Chemical-shift differences corresponding 1H 13C resonances D- L-phenylglycine (PG) or phenylglycine...
ADVERTISEMENT RETURN TO ISSUEPREVNoteNEXTSynthesis of Optically Active Phthaloyl d-Aminooxy Acids from l-Amino or l-Hydroxy as Building Blocks for the Preparation Aminooxy PeptidesInjae Shin, Mung-ryul Lee, Jiyong Mankil Jung, Weontae and Jongbok YoonView Author Information Department Chemistry Biochemistry, Yonsei University, Seoul 120-749, Korea [email protected]Cite this: J. Org. Chem. 2000, 65, 22, 7667–7675Publication Date (Web):October 4, 2000Publication History Received27 April...
Two-component signal transduction systems, commonly found in prokaryotes, typically regulate cellular functions response to environmental conditions through a phosphorylation-dependent process. A new type of regulator, hp1043 (HP-RR) from Helicobacter pylori, has been recently identified. HP-RR is essential for cell growth and does not require the well known phosphorelay scheme. Unphosphorylated binds specifically its own promoter (P(1043)) autoregulates tlpB gene (P(tlpB)). We have...