A5054890896- Magnetic properties of thin films
- Receptor Mechanisms and Signaling
- Enzyme Structure and Function
- Advanced Electron Microscopy Techniques and Applications
- Lipid Membrane Structure and Behavior
- Genomics and Chromatin Dynamics
- Quantum and electron transport phenomena
- Physics of Superconductivity and Magnetism
- Sphingolipid Metabolism and Signaling
- Biochemical and Molecular Research
- Neuroscience and Neuropharmacology Research
- Neuropeptides and Animal Physiology
- Metallic Glasses and Amorphous Alloys
- Photosynthetic Processes and Mechanisms
- Chromosomal and Genetic Variations
- DNA Repair Mechanisms
- Electron and X-Ray Spectroscopy Techniques
- Magnetic Properties and Applications
- Shape Memory Alloy Transformations
- Photoreceptor and optogenetics research
- Phytase and its Applications
- Signaling Pathways in Disease
- Porphyrin Metabolism and Disorders
- Advanced biosensing and bioanalysis techniques
- Neural dynamics and brain function
First Affiliated Hospital of Guangzhou University of Chinese Medicine
2023
FirstUnion (China)
2023
Arizona State University
2003-2022
University College London
2022
University of Essex
2020
Chinese Academy of Sciences
2005-2019
Hefei Institutes of Physical Science
2019
Institute of Biophysics
2014
University of Chinese Academy of Sciences
2014
National Chung Cheng University
2002
In higher eukaryotes, the centromere is epigenetically specified by histone H3 variant Centromere Protein-A (CENP-A). Deposition of CENP-A to requires chaperone HJURP (Holliday junction recognition protein). The crystal structure an HJURP–CENP-A–histone H4 complex shows that binds a CENP-A–H4 heterodimer. C-terminal β-sheet domain caps DNA-binding region heterodimer, preventing it from spontaneous association with DNA. Our analysis also revealed novel site in distinguishes its ability bind...
Two distinct antagonist-bound structures of CysLT 1 R reveal unique ligand-binding modes and signaling mechanisms.
Since the first successful serial crystallography (SX) experiment at a synchrotron radiation source, popularity of this approach has continued to grow showing that third-generation synchrotrons can be viable alternatives scarce X-ray free-electron laser sources. Synchrotron flux may increased ∼100 times by moderate increase in bandwidth ('pink beam' conditions) some cost data analysis complexity. Here, we report high-viscosity injector-based pink-beam SX experiments. The structures...
Significance Light-driven rhodopsin proteins pump ions across cell membranes. They have applications in optogenetics and can potentially be used to develop solar energy–harvesting devices. A detailed understanding of dynamics functions may therefore assist research medicine, health, clean energy. This time-resolved crystallography study carried out with X-ray free-electron lasers reveals chloride ion–pumping (ClR) within 100 ps light activation. It shows the dissociation Cl − from Schiff...
Serial femtosecond crystallography (SFX) with X-ray free electron lasers (XFELs) allows structure determination of membrane proteins and time-resolved crystallography. Common liquid sample delivery continuously jets the protein crystal suspension into path XFEL, wasting a vast amount due to pulsed nature all current XFEL sources. The European (EuXFEL) delivers (fs) pulses in trains spaced 100 ms apart whereas within are currently separated by 889 ns. Therefore, continuous via fast wastes...
In eukaryotes, chromosomal processes are usually modulated through chromatin-modifying complexes that dynamically targeted to specific regions of chromatin. this study, we show the chromatin-remodeling complex SWR1 (SWR1-C) uses a distinct strategy regulate heterochromatin spreading. Swr1 binds in stable manner near prepare for H2A.Z deposition, which can be triggered by NuA4-mediated acetylation histone H4. We also demonstrate experiments with Swc4, module shared NuA4 and SWR1-C, coupled...
Hollow spherical particles of Ni, CoFe, the ferromagnetic shape memory alloy Ni49Mn30Ga21, and giant magnetostrictive Fe83Ga17, with diameters up to several tens microns were produced by spark erosion, using liquid nitrogen as dielectric liquid. In contrast, primarily solid when was argon. The wall thicknesses hollow depended on elemental composition. Different models are considered account for formation spark-eroded spheres, some potential benefits be derived from their use described.
The bioactive lysophospholipid sphingosine-1-phosphate (S1P) acts via five different subtypes of S1P receptors (S1PRs) - S1P1-5. S1P5 is predominantly expressed in nervous and immune systems, regulating the egress natural killer cells from lymph nodes playing a role neurodegenerative disorders, as well carcinogenesis. Several S1PR therapeutic drugs have been developed to treat these diseases; however, they lack receptor subtype selectivity, which leads side effects. In this article, we...
Significance Many amphipathic lipids, including cannabinoids, lysophospholipids, prostanoids, and leukotrienes, bind to G protein–coupled receptors (GPCRs) through the lipid bilayer. How these ligands get anchored their enter ligand-binding pockets is largely elusive. Our results revealed a distinctive recognition mechanism for prostaglandin D 2 (PGD ) receptor CRTH2 that not shared by other prostanoid receptors. Based on structural analysis, we propose two representative binding modes of...
Ni/sub 49/Mn/sub 30/Ga/sub 21/ fine particles were prepared by spark erosion. Most of the are spheres with sizes ranging from about 5 nm to 100 /spl mu/m. The have martensite structure at room temperature as indicated X-ray diffraction (XRD). Measurements dependence magnetization and high-temperature XRD patterns demonstrated that undergo a rarr/ austenite phase transformation in range 35/spl deg/C-70/spl deg/C during heating, sim/10/spl hysteresis for upon cooling.
We report a study of the magnetic switching behavior nanoscale Co rings using off-axis electron holography. Arrays 10nm thick polycrystalline with 400nm outer diameter (OD) and different inner (ID) were fabricated by electron-beam lithography. The was studied for OD∕ID ratios, two kinds reversal mechanism identified. For 400nm∕250nm 400nm∕50nm, started from so-called onion (bidomain) state, proceeding to stable vortex finally reversed state. intermediate 400nm∕150nm, instead accomplished via...
μNS is a 70 kDa major nonstructural protein of avian reoviruses, which cause significant economic losses in the poultry industry. They replicate inside viral factories host cells, and has been suggested to be minimal factor required for factory formation. Thus, determining structure great importance understanding its role infection. In study presented here, fragment consisting residues 448–605 was expressed as an EGFP fusion Sf9 insect cells. EGFP-μNS (448–605) crystallization cells...
Depressive disorder is defined as a psychiatric disease characterized by the core symptoms of anhedonia and learned helplessness. Currently, treatment depression still calls for medications with high effectiveness, rapid action, few side effects, although many drugs, including fluoxetine ketamine, have been approved clinical usage Food Drug Administration (FDA). In this study, we focused on calcitonin an amylin receptor polypeptide, which antidepressant effect has not reported, even if...
The effect of size and shape on the remanent states switching behavior for slotted Co disks rings has been determined using off-axis electron holography. Both shapes have very stable well-defined states, unlike complete with same dimensions. Slotted vortex during between two single domain whereas usually show rapid without formation. field range a ring, typically $\ensuremath{\sim}40\phantom{\rule{0.3em}{0ex}}\mathrm{Oe}$, is much smaller than not dependent open slot angle or outer diameter....
This study aimed to investigate the transport capability of nicotine across Calu-3 cell monolayer in various forms, including freebase, salts, and flavored e-liquids with benzoate.Nicotine is rapidly absorbed from respiratory system into systemic circulation during e-cigarettes use. However, mechanism lung has not been well understood yet. may offer critical biological evidence have implications for use regulation e-cigarettes.The viability cells after administration salts representative...
Septin 1 is a member of an evolutionarily conserved family GTP-binding and filament-forming proteins named septins, which function in diverse processes including cytokinasis, vesicle trafficking, apoptosis, remodelling the cytoskeleton, infection, neurodegeneration neoplasia. Human septin has been expressed purified, but suffers from severe aggregation. Studies have shown that with site-directed mutations five serine residues (Ser19, Ser206, Ser307, Ser312 Ser315) much lower degree...
The increase in successful adaptations of serial crystallography at synchrotron radiation sources continues. To date, the number (SSX) experiments has grown exponentially, with over 40 reported so far. In this work, we report first SSX viscous jets conducted ALBA beamline BL13-XALOC. Small crystals (15–30 µm) five soluble proteins (lysozyme, proteinase K, phycocyanin, insulin and α-spectrin-SH3 domain) were suspended lipidic cubic phase (LCP) delivered to X-ray beam a high-viscosity injector...