A5078321549- Enzyme Structure and Function
- Protein Structure and Dynamics
- RNA and protein synthesis mechanisms
- Bacillus and Francisella bacterial research
- Viral Infectious Diseases and Gene Expression in Insects
- Biochemical and Molecular Research
- Yersinia bacterium, plague, ectoparasites research
- SARS-CoV-2 and COVID-19 Research
- Bacteriophages and microbial interactions
- Computational Drug Discovery Methods
- Bacterial Genetics and Biotechnology
- Viral gastroenteritis research and epidemiology
- Transgenic Plants and Applications
- Cancer Research and Treatments
- Mosquito-borne diseases and control
- Skin Diseases and Diabetes
- Rabies epidemiology and control
- Venomous Animal Envenomation and Studies
- Autoimmune and Inflammatory Disorders Research
- Nuclear Physics and Applications
- Insect Resistance and Genetics
- Malaria Research and Control
- Porphyrin Metabolism and Disorders
- ATP Synthase and ATPases Research
- Enzyme Production and Characterization
Arizona State University
2016-2024
University of Colombo
1959-2014
Ever since the first atomic structure of an enzyme was solved, discovery mechanism and dynamics reactions catalyzed by biomolecules has been key goal for understanding molecular processes that drive life on earth. Despite a large number successful methods trapping reaction intermediates, direct observation ongoing possible only in rare exceptional cases.
Crystal structure determination of biological macromolecules using the novel technique serial femtosecond crystallography (SFX) is severely limited by scarcity X-ray free-electron laser (XFEL) sources. However, recent and future upgrades render microfocus beamlines at synchrotron-radiation sources suitable for room-temperature data collection also. Owing to longer exposure times that are needed synchrotrons, termed millisecond (SMX). As a result, number SMX experiments growing rapidly, with...
Mix-and-inject serial crystallography (MISC) is a technique designed to image enzyme catalyzed reactions in which small protein crystals are mixed with substrate just prior being probed by an X-ray pulse. This approach offers several advantages over flow cell studies. It provides (i) room temperature structures at near atomic resolution, (ii) time resolution ranging from microseconds seconds, and (iii) convenient reaction initiation. outruns radiation damage using femtosecond pulses allowing...
Baculovirus mediated-insect cell expression systems have been widely used for producing heterogeneous proteins. However, to date, there is still the lack of an easy-to-manipulate system that enables high-throughput protein characterization in insect cells by taking advantage large existing Gateway clone libraries. To resolve this limitation, we constructed a suite Gateway-compatible pIEx-derived baculovirus vectors allow rapid and cost-effective construction clones mass parallel cells. This...
μNS is a 70 kDa major nonstructural protein of avian reoviruses, which cause significant economic losses in the poultry industry. They replicate inside viral factories host cells, and has been suggested to be minimal factor required for factory formation. Thus, determining structure great importance understanding its role infection. In study presented here, fragment consisting residues 448–605 was expressed as an EGFP fusion Sf9 insect cells. EGFP-μNS (448–605) crystallization cells...
Francisella tularensis is an extremely infectious pathogen and a category A bioterrorism agent. It causes the highly contagious zoonosis, Tularemia. Currently, FDA approved vaccines against tularemia are unavailable. F. outer membrane protein (FopA) well-studied virulence determinant protective antigen tularemia. major (Omp) of tularensis. However, FopA-based therapeutic intervention hindered due to lack complete structural information for localized mature FopA. In our study, we established...
Human lymphatic filariasis (HLF) is a neglected tropical disease which threatens nearly 1.4 billion people in 73 countries worldwide. Wuchereria bancrofti the major causative agent of HLF and it closely resembles cattle filarial parasite Setaria digitata. Due to difficulties procuring W. material, S. digitata cDNA library has been constructed identify novel drug targets against many sequences are yet be assigned structure function. In this study, 549 bp long (sdrbp) sequenced characterized...
NendoU from SARS-CoV-2 is responsible for the virus's ability to evade innate immune system by cleaving poly-uridine leader sequence of antisense viral RNA.Using serial femtosecond crystallography (SFX) at an X-ray free electron laser, room temperature structure was solved 2.6 Å resolution MFX endstation Linac Coherent Light Source in California, USA.The protein crystals were injected using electro-spinning jet produced microfluidic electrokinetic sample holder (MESH) injector, as well through a gas
Ever since the first atomic structure of an enzyme was solved, discovery mechanism and dynamics reactions catalyzed by biomolecules has been key goal for understanding molecular processes that drive life on earth. Despite a large number successful methods trapping reaction intermediates, direct observation ongoing possible only in rare exceptional cases. Here, we demonstrate general method capturing catalysis ‘in-action’ ‘mix-and-inject serial crystallography’. Specifically, follow catalytic...
Abstract Francisella tularensis is an extremely infectious pathogen and a category A bioterrorism agent. It causes the highly contagious zoonosis, Tularemia. Currently, FDA approved vaccines against tularemia are unavailable. F. outer membrane protein (FopA) well-studied virulence determinant protective antigen tularemia. major (Omp) of . However, FopA-based therapeutic intervention hindered due to lack complete structural information for localized mature FopA. In our study, we established...
Ten cases of active chronic hepatitis in Sri Lanka patients are presented. Their clinical and biochemical findings were no way different from previously published cases. Two followed an attack viral hepatitis. Three had nutritional deficiencies, all taken Ayurvedic decoctions at some stage their illness. Four raised globulins differences hypergammaglobulinaemia. The histological appearances that seen other reports but the majority also marked fatty change. There appeared to be likely...