Petra Fromme
A5076993754- Photosynthetic Processes and Mechanisms
- Enzyme Structure and Function
- Photoreceptor and optogenetics research
- Advanced Electron Microscopy Techniques and Applications
- Advanced X-ray Imaging Techniques
- Spectroscopy and Quantum Chemical Studies
- Protein Structure and Dynamics
- Mitochondrial Function and Pathology
- Biochemical and Molecular Research
- Receptor Mechanisms and Signaling
- Crystallization and Solubility Studies
- Algal biology and biofuel production
- Mass Spectrometry Techniques and Applications
- ATP Synthase and ATPases Research
- RNA and protein synthesis mechanisms
- X-ray Spectroscopy and Fluorescence Analysis
- Light effects on plants
- Bacteriophages and microbial interactions
- Metal-Catalyzed Oxygenation Mechanisms
- Microfluidic and Capillary Electrophoresis Applications
- Porphyrin and Phthalocyanine Chemistry
- Metalloenzymes and iron-sulfur proteins
- Origins and Evolution of Life
- X-ray Diffraction in Crystallography
- Advanced Fluorescence Microscopy Techniques
Arizona State University
2016-2025
Argonne National Laboratory
2016-2019
Max Planck Institute for Nuclear Physics
2018
Universität Hamburg
2016
Deutsches Elektronen-Synchrotron DESY
2016
Center for Free-Electron Laser Science
2016
Physical Sciences (United States)
2013-2014
Statistics Finland
2012
European Synchrotron Radiation Facility
2008
Lawrence Berkeley National Laboratory
2002-2006
Size Matters Less X-ray crystallography is a central research tool for uncovering the structures of proteins and other macromolecules. However, its applicability typically requires growth large crystals, in part because sufficient number molecules must be present lattice sample to withstand x-ray—induced damage. Boutet et al. (p. 362 , published online 31 May) now demonstrate that intense x-ray pulses emitted by free-electron laser source can yield data few enough exposures uncover...
Serial femtosecond crystallography using ultrashort pulses from x-ray free electron lasers (XFELs) enables studies of the light-triggered dynamics biomolecules. We used microcrystals photoactive yellow protein (a bacterial blue light photoreceptor) as a model system and obtained high-resolution, time-resolved difference density maps excellent quality with strong features; these allowed determination structures reaction intermediates to resolution 1.6 angstroms. Our results open way study...
X-ray crystallography of G protein-coupled receptors and other membrane proteins is hampered by difficulties associated with growing sufficiently large crystals that withstand radiation damage yield high-resolution data at synchrotron sources. We used an x-ray free-electron laser (XFEL) individual 50-femtosecond-duration pulses to minimize obtained a room-temperature structure human serotonin receptor using sub-10-micrometer microcrystals grown in mimetic matrix known as lipidic cubic phase....
The Trypanosoma brucei cysteine protease cathepsin B (TbCatB), which is involved in host protein degradation, a promising target to develop new treatments against sleeping sickness, fatal disease caused by this protozoan parasite. structure of the mature, active form TbCatB has so far not provided sufficient information for design safe and specific drug T. brucei. By combining two recent innovations, vivo crystallization serial femtosecond crystallography, we obtained room-temperature 2.1...
Visualizing a response to light Many biological processes depend on detecting and responding light. The is often mediated by structural change in protein that begins when absorption of photon causes isomerization chromophore bound the protein. Pande et al. used x-ray pulses emitted free electron laser source conduct time-resolved serial femtosecond crystallography time range 100 fs 3 ms. This allowed for real-time tracking trans-cis photoactive yellow associated changes Science , this issue p. 725
We demonstrate the use of an X-ray free electron laser synchronized with optical pump to obtain diffraction snapshots from photoactivated states large membrane protein complexes in form nanocrystals flowing a liquid jet.Light-induced changes Photosystem I-Ferredoxin co-crystals were observed at time delays 5 10 µs after excitation.The result correlates microsecond kinetics transfer I ferredoxin.The undocking process that follows leads rearrangements crystals will terminally lead...
Abstract The new European X-ray Free-Electron Laser is the first free-electron laser capable of delivering pulses with a megahertz inter-pulse spacing, more than four orders magnitude higher previously possible. However, to date, it has been unclear whether would indeed be possible measure high-quality diffraction data at pulse repetition rates. Here, we show that structures can obtained using currently available operating conditions XFEL. We present two complete sets, one from well-known...
We observe previously unknown interactions between clinically important adenovirus vector capsids, platelet factor 4, and CAR.