Jason E. Koglin
A5055583239- Enzyme Structure and Function
- Advanced X-ray Imaging Techniques
- Particle Detector Development and Performance
- Advanced Electron Microscopy Techniques and Applications
- Dark Matter and Cosmic Phenomena
- Astrophysical Phenomena and Observations
- Photosynthetic Processes and Mechanisms
- X-ray Spectroscopy and Fluorescence Analysis
- Particle physics theoretical and experimental studies
- Photoreceptor and optogenetics research
- Spectroscopy and Quantum Chemical Studies
- Laser-Plasma Interactions and Diagnostics
- Astrophysics and Cosmic Phenomena
- Gamma-ray bursts and supernovae
- Atomic and Subatomic Physics Research
- Nuclear Physics and Applications
- Laser-Matter Interactions and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Neutrino Physics Research
- Advanced Chemical Physics Studies
- Near-Field Optical Microscopy
- Atomic and Molecular Physics
- Advanced Fluorescence Microscopy Techniques
- Astronomy and Astrophysical Research
- Particle Accelerators and Free-Electron Lasers
Linac Coherent Light Source
2015-2025
SLAC National Accelerator Laboratory
2016-2025
Los Alamos National Laboratory
2021-2024
Voss Scientific (United States)
2022
Fermi National Accelerator Laboratory
2021
Menlo School
2013-2019
Coherent (United States)
2017
SRI International
2017
European X-Ray Free-Electron Laser
2017
Brookhaven National Laboratory
2017
The Nuclear Spectroscopic Telescope Array (NuSTAR) mission, launched on 2012 June 13, is the first focusing high-energy X-ray telescope in orbit. NuSTAR operates band from 3 to 79 keV, extending sensitivity of far beyond ∼10 keV cutoff achieved by all previous satellites. inherently low background associated with concentrating light enables probe hard sky a more than 100-fold improvement over collimated or coded mask instruments that have operated this bandpass. Using its unprecedented...
Serial femtosecond crystallography using ultrashort pulses from x-ray free electron lasers (XFELs) enables studies of the light-triggered dynamics biomolecules. We used microcrystals photoactive yellow protein (a bacterial blue light photoreceptor) as a model system and obtained high-resolution, time-resolved difference density maps excellent quality with strong features; these allowed determination structures reaction intermediates to resolution 1.6 angstroms. Our results open way study...
X-ray crystallography of G protein-coupled receptors and other membrane proteins is hampered by difficulties associated with growing sufficiently large crystals that withstand radiation damage yield high-resolution data at synchrotron sources. We used an x-ray free-electron laser (XFEL) individual 50-femtosecond-duration pulses to minimize obtained a room-temperature structure human serotonin receptor using sub-10-micrometer microcrystals grown in mimetic matrix known as lipidic cubic phase....
Visualizing a response to light Many biological processes depend on detecting and responding light. The is often mediated by structural change in protein that begins when absorption of photon causes isomerization chromophore bound the protein. Pande et al. used x-ray pulses emitted free electron laser source conduct time-resolved serial femtosecond crystallography time range 100 fs 3 ms. This allowed for real-time tracking trans-cis photoactive yellow associated changes Science , this issue p. 725
Intense femtosecond x-ray pulses produced at the Linac Coherent Light Source (LCLS) were used for simultaneous diffraction (XRD) and emission spectroscopy (XES) of microcrystals photosystem II (PS II) room temperature. This method probes overall protein structure electronic Mn4CaO5 cluster in oxygen-evolving complex PS II. XRD data are presented from both dark state (S1) first illuminated (S2) Our XRD-XES study shows that crystals intact during our measurements LCLS, not only with respect to...
The hemoprotein myoglobin is a model system for the study of protein dynamics. We used time-resolved serial femtosecond crystallography at an x-ray free-electron laser to resolve ultrafast structural changes in carbonmonoxy complex upon photolysis Fe-CO bond. Structural appear throughout within 500 femtoseconds, with C, F, and H helices moving away from heme cofactor E A toward it. These collective movements are predicted by hybrid quantum mechanics/molecular mechanics simulations. Together...
Look fast Organisms from bacteria to humans sense and react light. Proteins that contain the light-sensitive molecule retinal couple absorption of light conformational changes produce a signal or move ions across membrane. Nogly et al. used an x-ray laser probe earliest structural chromophore within microcrystals ion pump bacteriorhodopsin (see Perspective by Moffat). The excited-state wiggles but is held in place so only one double bond capable isomerizing. A water adjacent proton-pumping...
Abstract Bacteriorhodopsin (bR) is a light-driven proton pump. The primary photochemical event upon light absorption isomerization of the retinal chromophore. Here we used time-resolved crystallography at an X-ray free-electron laser to follow structural changes in multiphoton-excited bR from 250 femtoseconds 10 picoseconds. Quantum chemistry and ultrafast spectroscopy were identify sequential two-photon process, leading excitation tryptophan residue flanking chromophore, as first...
We report the results of a multi-band observing campaign on famous blazar 3C 279 conducted during phase increased activity from 2013 December to 2014 April, including first observations it with NuSTAR. The $\gamma$-ray emission source measured by Fermi-LAT showed multiple distinct flares reaching highest flux level in this object since beginning Fermi mission, $F(E > 100\,{\rm MeV})$ $10^{-5}$ photons cm$^{-2}$ s$^{-1}$, and doubling time scale as short 2 hours. spectrum one was very hard,...
The Coherent X-ray Imaging (CXI) instrument specializes in hard X-ray, in-vacuum, high power density experiments all areas of science. Two main sample chambers, one containing a 100 nm focus and 1 µm focus, are available, each with multiple diagnostics, injection, pump-probe detector capabilities. flexibility CXI has enabled it to host diverse range experiments, from biological extreme matter.
Ever since the first atomic structure of an enzyme was solved, discovery mechanism and dynamics reactions catalyzed by biomolecules has been key goal for understanding molecular processes that drive life on earth. Despite a large number successful methods trapping reaction intermediates, direct observation ongoing possible only in rare exceptional cases.
Serial femtosecond crystallography (SFX) has opened a new era in by permitting nearly damage-free, room-temperature structure determination of challenging proteins such as membrane proteins. In SFX, X-ray free-electron laser pulses produce diffraction snapshots from nanocrystals and microcrystals delivered liquid jet, which leads to high protein consumption. A slow-moving stream agarose been developed crystal delivery medium for SFX. It low background scattering, is compatible with both...
Stellar metals shine toward our eyes only Taking a different look at familiar star may still yield surprises. Boggs et al. trained the x-ray vision of NuSTAR observatory on well-studied supernova 1987A. Core-collapse explosions such as SN 1987A produce titanium isotope, 44 Ti, whose radioactive decay yields hard emission lines. All associated with 44Ti appears to be from material moving us, none away. This implies that explosion was not symmetric. These findings help explain mechanics and...