M. Messerschmidt
A5038083997- Advanced X-ray Imaging Techniques
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Advanced Electron Microscopy Techniques and Applications
- Enzyme Structure and Function
- X-ray Spectroscopy and Fluorescence Analysis
- Particle Accelerators and Free-Electron Lasers
- Laser-Plasma Interactions and Diagnostics
- Crystallography and molecular interactions
- Laser-Matter Interactions and Applications
- Advanced Chemical Physics Studies
- Photosynthetic Processes and Mechanisms
- Crystallography and Radiation Phenomena
- Spectroscopy and Quantum Chemical Studies
- Particle accelerators and beam dynamics
- Receptor Mechanisms and Signaling
- Electron and X-Ray Spectroscopy Techniques
- Protein Structure and Dynamics
- Laser-induced spectroscopy and plasma
- Atomic and Molecular Physics
- Ion-surface interactions and analysis
- Biochemical and Molecular Research
- Magnetism in coordination complexes
- Advanced Surface Polishing Techniques
- High-pressure geophysics and materials
Arizona State University
2019-2022
Zentrum für Sonnenenergie- und Wasserstoff-Forschung Baden-Württemberg
2014-2022
Linac Coherent Light Source
2011-2020
SLAC National Accelerator Laboratory
2011-2020
U.S. National Science Foundation
2015-2020
BioXFEL Science and Technology Center
2015-2020
European X-Ray Free-Electron Laser
2017-2020
Micro Resist Technology (Germany)
2020
Menlo School
2008-2017
SRI International
2017
Size Matters Less X-ray crystallography is a central research tool for uncovering the structures of proteins and other macromolecules. However, its applicability typically requires growth large crystals, in part because sufficient number molecules must be present lattice sample to withstand x-ray—induced damage. Boutet et al. (p. 362 , published online 31 May) now demonstrate that intense x-ray pulses emitted by free-electron laser source can yield data few enough exposures uncover...
X-ray crystallography of G protein-coupled receptors and other membrane proteins is hampered by difficulties associated with growing sufficiently large crystals that withstand radiation damage yield high-resolution data at synchrotron sources. We used an x-ray free-electron laser (XFEL) individual 50-femtosecond-duration pulses to minimize obtained a room-temperature structure human serotonin receptor using sub-10-micrometer microcrystals grown in mimetic matrix known as lipidic cubic phase....
The Trypanosoma brucei cysteine protease cathepsin B (TbCatB), which is involved in host protein degradation, a promising target to develop new treatments against sleeping sickness, fatal disease caused by this protozoan parasite. structure of the mature, active form TbCatB has so far not provided sufficient information for design safe and specific drug T. brucei. By combining two recent innovations, vivo crystallization serial femtosecond crystallography, we obtained room-temperature 2.1...
Intense femtosecond x-ray pulses produced at the Linac Coherent Light Source (LCLS) were used for simultaneous diffraction (XRD) and emission spectroscopy (XES) of microcrystals photosystem II (PS II) room temperature. This method probes overall protein structure electronic Mn4CaO5 cluster in oxygen-evolving complex PS II. XRD data are presented from both dark state (S1) first illuminated (S2) Our XRD-XES study shows that crystals intact during our measurements LCLS, not only with respect to...
Distorted Nanoparticle Nanoparticles have found many applications in modern technology; however, the full characterization of individual particles is challenging. One most interesting mechanical properties particle's response to lattice distortion. This property has been probed for ensembles nanoparticles, but required averaging may distort results. Clark et al. (p. 56 , published online 23 May; see Perspective by Hartland and Lo ) were able image generation subsequent evolution coherent...
We have used the Linac Coherent Light Source to generate solid-density aluminum plasmas at temperatures of up 180 eV. By varying photon energy x rays that both create and probe plasma, observing K-α fluorescence, we can directly measure position K edge highly charged ions within system. The results are found disagree with predictions extensively Stewart-Pyatt model, but consistent earlier model Ecker Kröll, which predicts significantly greater depression ionization potential.
The nonlinear absorption mechanisms of neon atoms to intense, femtosecond kilovolt x rays are investigated. production Ne(9+) is observed at x-ray frequencies below the Ne(8+), 1s(2) edge and demonstrates a clear quadratic dependence on fluence. Theoretical analysis shows that combination two-photon ionization Ne(8+) ground state high-order sequential process involving single-photon transient excited states time scale faster than Auger decay. We find direct cross section orders magnitude...
We demonstrate the use of an X-ray free electron laser synchronized with optical pump to obtain diffraction snapshots from photoactivated states large membrane protein complexes in form nanocrystals flowing a liquid jet.Light-induced changes Photosystem I-Ferredoxin co-crystals were observed at time delays 5 10 µs after excitation.The result correlates microsecond kinetics transfer I ferredoxin.The undocking process that follows leads rearrangements crystals will terminally lead...
We report experimental results on x-ray diffraction of quantum-state-selected and strongly aligned ensembles the prototypical asymmetric rotor molecule 2,5-diiodobenzonitrile using Linac Coherent Light Source. The experiments demonstrate first steps toward a new approach to diffractive imaging distinct structures individual, isolated gas-phase molecules. confirm several key ingredients single experiments: abilities detect count individual scattered photons in shot data, deliver...
With an eye toward extending optical wave-mixing techniques to the x-ray regime, we present first experimental demonstration of a two-color free-electron laser at Linac Coherent Light Source. We combine emittance-spoiler technique with magnetic chicane in undulator section control pulse duration and relative delay between two intense pulses use differently tuned canted pole undulators such that have different wavelengths as well. Two schemes are shown produce soft wavelength separation up...