Miriam Barthelmeß
A5023218258- Advanced X-ray Imaging Techniques
- Advanced Electron Microscopy Techniques and Applications
- Enzyme Structure and Function
- X-ray Spectroscopy and Fluorescence Analysis
- Particle Accelerators and Free-Electron Lasers
- Magnetic properties of thin films
- Crystallography and Radiation Phenomena
- Protein Structure and Dynamics
- Computational Drug Discovery Methods
- Laser-Matter Interactions and Applications
- Photosynthetic Processes and Mechanisms
- Laser-Plasma Interactions and Diagnostics
- Mass Spectrometry Techniques and Applications
- Magnetic Properties and Applications
- SARS-CoV-2 and COVID-19 Research
- X-ray Diffraction in Crystallography
- Diamond and Carbon-based Materials Research
- Advanced Chemical Physics Studies
- Digital Holography and Microscopy
- nanoparticles nucleation surface interactions
- Astrophysical Phenomena and Observations
- Crystallization and Solubility Studies
- RNA and protein synthesis mechanisms
- Quantum and electron transport phenomena
- Theoretical and Computational Physics
Deutsches Elektronen-Synchrotron DESY
2007-2023
Universität Hamburg
2004-2023
Center for Free-Electron Laser Science
2013-2023
Max Planck Institute for the Structure and Dynamics of Matter
2015-2018
University of Toronto
2018
RWTH Aachen University
2018
University of Minnesota
2017
Australian National University
2015
European X-Ray Free-Electron Laser
2015
Arizona State University
2015
The coronavirus disease (COVID-19) caused by SARS-CoV-2 is creating tremendous human suffering. To date, no effective drug available to directly treat the disease. In a search for against COVID-19, we have performed high-throughput x-ray crystallographic screen of two repurposing libraries main protease (M
We demonstrate the use of an X-ray free electron laser synchronized with optical pump to obtain diffraction snapshots from photoactivated states large membrane protein complexes in form nanocrystals flowing a liquid jet.Light-induced changes Photosystem I-Ferredoxin co-crystals were observed at time delays 5 10 µs after excitation.The result correlates microsecond kinetics transfer I ferredoxin.The undocking process that follows leads rearrangements crystals will terminally lead...
X-ray free-electron lasers deliver intense femtosecond pulses that promise to yield high resolution diffraction data of nanocrystals before the destruction sample by radiation damage. Diffraction intensities lysozyme collected at Linac Coherent Light Source using 2 keV photons were used for structure determination molecular replacement and analyzed damage as a function pulse length fluence. Signatures are observed short 70 fs. Parametric scaling in conventional crystallography does not...
Serial X-ray crystallography allows macromolecular structure determination at both free electron lasers (XFELs) and, more recently, synchrotron sources. The time resolution for serial experiments has been limited to millisecond timescales with monochromatic beams. polychromatic, "pink", beam provides a than two orders of magnitude increased photon flux and hence accessing much shorter in diffraction Here we report the different protein samples by merging pink-beam patterns from many...
Abstract Serial femtosecond crystallography requires reliable and efficient delivery of fresh crystals across the beam an X-ray free-electron laser over course experiment. We introduce a double-flow focusing nozzle to meet this challenge, with significantly reduced sample consumption, while improving jet stability previous generations nozzles. demonstrate its use determine first room-temperature structure RNA polymerase II at high resolution, revealing new structural details. Moreover,...
Abstract The ever-increasing brightness of synchrotron radiation sources demands improved X-ray optics to utilise their capability for imaging and probing biological cells, nanodevices functional matter on the nanometer scale with chemical sensitivity. Here we demonstrate focusing a hard beam an 8 nm focus using volume zone plate (also referred as wedged multilayer Laue lens). This lens was constructed new deposition technique that enabled independent control angle thickness diffracting...
Single-particle experiments using X-ray Free Electron Lasers produce more than 10(5) snapshots per hour, consisting of an admixture blank shots (no particle intercepted), and exposures one or particles. Experimental data sets also often contain unintentional contamination with different species. We present unsupervised method able to sort experimental without recourse templates, specific noise models, user-directed learning. The results show 90% agreement manual classification.
The resolution of single-shot coherent diffractive imaging at X-ray free-electron laser facilities is limited by the low signal-to-noise level diffraction data high scattering angles.The iterative reconstruction methods, which phase a continuous pattern to produce an image, must be able extract information from these weak signals obtain best quality images.Here we show how modify methods improve tolerance noise.The method demonstrated with hybrid input-output on both simulated and patterns...
FERMI@Elettra, the first vacuum ultraviolet and soft X-ray free-electron laser (FEL) using by default a "seeded" scheme, became operational in 2011 has been opened to users since December 2012. The parameters of seeded FERMI FEL pulses and, particular, superior control emitted radiation terms spectral purity stability meet stringent requirements for single-shot resonant coherent diffraction imaging (CDI) experiments. advantages intense with variable polarization have demonstrated experiments...
Efficient and reliable sample delivery has remained one of the bottlenecks for serial crystallography experiments. Compared with other methods, fixed-target offers advantage significantly reduced consumption shorter data collection times owing to higher hit rates. Here, a new method on-chip crystallization is reported which allows efficient reproducible growth large numbers protein crystals directly on micro-patterned silicon chips in-situ Crystals are grown by sitting-drop vapor diffusion...
We demonstrate that x-ray fluorescence emission, which cannot maintain a stationary interference pattern, can be used to obtain images of structures by recording photon-photon correlations in the manner stellar intensity interferometry Hanbury Brown and Twiss. This is achieved utilizing femtosecond-duration pulses hard free-electron laser generate emission exposures comparable coherence time fluorescence. Iterative phasing photon correlation map generated model-free real-space image...
We describe femtosecond X-ray diffraction data sets of viruses and nanoparticles collected at the Linac Coherent Light Source.The establish first large benchmark for coherent methods freely available to public, bolster development algorithms that are essential developing this novel approach as a useful imaging technique.Applications 2D reconstructions, orientation classification finally 3D by assembling patterns into volume.
Wavelength, reflectance, and stress stability of Mo/B4C multilayers were studied as a function postdeposition annealing up to 900 °C. These are interest normal incidence coatings for wavelengths above the boron K-absorption edge. deposited at low sputtering pressure have high compressive stress. Zero can be achieved 360 °C–370 °C, but <200 °C is sufficient reduce by ∼40%. This relaxation accompanied with multilayer period expansion ∼0.02 nm <0.5% decrease in reflectivity. The remains stable...
The routine atomic resolution structure determination of single particles is expected to have profound implications for probing structure-function relationships in systems ranging from energy-storage materials biological molecules. Extremely bright ultrashort-pulse X-ray sources - free-electron lasers (XFELs) provide X-rays that can be used probe ensembles nearly identical nanoscale particles. When combined with coherent diffractive imaging, these objects imaged; however, as the images...
Reliable sample delivery and efficient use of limited beam time have remained bottlenecks for serial crystallography (SX). Using a high-intensity polychromatic X-ray in combination with newly developed charge-integrating JUNGFRAU detector, we applied the method fixed-target SX to collect data at rate 1 kHz synchrotron-radiation facility. According our analysis given experimental conditions, only about 3 000 diffraction patterns are required high-quality dataset. With indexing rates up 25%,...
Here we present a new approach to diffraction imaging of amyloid fibrils, combining free-standing graphene support and single nanofocused X-ray pulses femtosecond duration from an free-electron laser. Due the very low background scattering mutual alignment filaments, tobacco mosaic virus (TMV) filaments protofibrils is obtained 2.7 Å 2.4 resolution in patterns, respectively. Some TMV patterns exhibit asymmetry that indicates presence limited number axial rotations XFEL focus. Signal-to-noise...
The emergence of femtosecond diffractive imaging with X-ray lasers has enabled pioneering structural studies isolated particles, such as viruses, at nanometer length scales.However, the issue missing low frequency data significantly limits potential to reveal sub-nanometer details micrometer-sized samples.We have developed a new technique dark-field coherent simultaneously overcome and enable us harness unique contrast mechanisms available in microscopy.Images airborne particulate matter...
The response of solids to electromagnetic fields is crucial importance in many areas science and technology.Many fundamental questions remain be answered about the dynamics photoexcited electrons that underpin this response, which can evolve on timescales tens hundreds attoseconds.How, for example, electron affected by periodic potential as it travels solid, how do other respond these strongly correlated systems?Furthermore, control electronic motion with attosecond precision would pave way...