Filipe R. N. C. Maia
A5057703441- Advanced X-ray Imaging Techniques
- Advanced Electron Microscopy Techniques and Applications
- X-ray Spectroscopy and Fluorescence Analysis
- Enzyme Structure and Function
- Laser-Plasma Interactions and Diagnostics
- Particle Accelerators and Free-Electron Lasers
- Crystallography and Radiation Phenomena
- Astrophysical Phenomena and Observations
- Plant Virus Research Studies
- Ion-surface interactions and analysis
- Medical Imaging Techniques and Applications
- Protein Structure and Dynamics
- nanoparticles nucleation surface interactions
- Bacteriophages and microbial interactions
- Digital Holography and Microscopy
- Advanced X-ray and CT Imaging
- Photosynthetic Processes and Mechanisms
- Electron and X-Ray Spectroscopy Techniques
- Viral Infections and Vectors
- Atomic and Subatomic Physics Research
- Electron Spin Resonance Studies
- Laser-Matter Interactions and Applications
- Atmospheric aerosols and clouds
- NF-κB Signaling Pathways
- Plant and Fungal Interactions Research
Lawrence Berkeley National Laboratory
2015-2024
National Energy Research Scientific Computing Center
2015-2024
Uppsala University
2015-2024
Institute of Molecular Biology and Biophysics
2023
Science for Life Laboratory
2008-2023
MAX IV Laboratory
2020
Lund University
2020
European X-Ray Free-Electron Laser
2015-2020
La Trobe University
2020
SLAC National Accelerator Laboratory
2007-2019
The emerging technique of serial X-ray diffraction, in which diffraction data are collected from samples flowing across a pulsed source at repetition rates 100 Hz or higher, has necessitated the development new software order to handle large volumes produced. Sorting according different criteria and rapid filtering events retain only patterns interest results significant reductions volume, thereby simplifying subsequent analysis management tasks. Meanwhile generation reduced form virtual...
We present a proof-of-concept three-dimensional reconstruction of the giant mimivirus particle from experimentally measured diffraction patterns an x-ray free-electron laser. Three-dimensional imaging requires assembly many two-dimensional into internally consistent Fourier volume. Since each is randomly oriented when exposed to pulse, relative orientations have be retrieved data alone. achieve this with modified version expand, maximize and compress algorithm validate our result using new...
We demonstrate the use of an X-ray free electron laser synchronized with optical pump to obtain diffraction snapshots from photoactivated states large membrane protein complexes in form nanocrystals flowing a liquid jet.Light-induced changes Photosystem I-Ferredoxin co-crystals were observed at time delays 5 10 µs after excitation.The result correlates microsecond kinetics transfer I ferredoxin.The undocking process that follows leads rearrangements crystals will terminally lead...
Helium nanodroplets are considered ideal model systems to explore quantum hydrodynamics in self-contained, isolated superfluids. However, exploring the dynamic properties of individual droplets is experimentally challenging. In this work, we used single-shot femtosecond x-ray coherent diffractive imaging investigate rotation single, superfluid helium-4 containing ~10(8) 10(11) atoms. The formation vortex lattices inside confirmed by observing characteristic Bragg patterns from xenon clusters...
Intense femtosecond x-ray pulses from free-electron laser sources allow the imaging of individual particles in a single shot. Early experiments at Linac Coherent Light Source (LCLS) have led to rapid progress field and, so far, coherent diffractive images been recorded biological specimens, aerosols, and quantum systems with few-tens-of-nanometers resolution. In March 2014, LCLS held workshop discuss scientific technical challenges for reaching ultimate goal atomic resolution single-shot...
Abstract The new European X-ray Free-Electron Laser is the first free-electron laser capable of delivering pulses with a megahertz inter-pulse spacing, more than four orders magnitude higher previously possible. However, to date, it has been unclear whether would indeed be possible measure high-quality diffraction data at pulse repetition rates. Here, we show that structures can obtained using currently available operating conditions XFEL. We present two complete sets, one from well-known...
There exists a conspicuous gap of knowledge about the organization life at mesoscopic levels. Ultra-fast coherent diffractive imaging with X-ray free-electron lasers can probe structures relevant length scales and may reach sub-nanometer resolution on micron-sized living cells. Here we show that introduce beam aerosolised cyanobacteria into focus Linac Coherent Light Source record diffraction patterns from individual cells very low noise levels high hit ratios. We obtain two-dimensional...
In nanotechnology, strategies for the creation and manipulation of nanoparticles in gas phase are critically important surface modification substrate-free characterization. Recent coherent diffractive imaging with intense femtosecond X-ray pulses has verified capability single-shot nanoscale objects at suboptical resolutions beyond radiation-induced damage threshold. By intercepting electrospray-generated particles a single 15 soft-X-ray pulse, we demonstrate specimen free flight first time,...
Coherent x-ray diffractive imaging is a powerful method for studies on nonperiodic structures the nanoscale. Access to femtosecond dynamics in major physical, chemical, and biological processes requires single-shot diffraction data. Up now, this has been limited intense coherent pulses from free electron laser. Here we show that laser-driven ultrashort sources offer comparatively inexpensive alternative. We present measurements of patterns isolated nano-objects with single 20 fs pulse...
X-ray free-electron lasers deliver intense femtosecond pulses that promise to yield high resolution diffraction data of nanocrystals before the destruction sample by radiation damage. Diffraction intensities lysozyme collected at Linac Coherent Light Source using 2 keV photons were used for structure determination molecular replacement and analyzed damage as a function pulse length fluence. Signatures are observed short 70 fs. Parametric scaling in conventional crystallography does not...
A complete set of structure factors has been extracted from hundreds thousands femtosecond single-shot X-ray microdiffraction patterns taken randomly oriented nanocrystals. The method Monte Carlo integration over crystallite size and orientation was applied to experimental data Photosystem I This arrives at many partial reflections without prior knowledge the particle-size distribution. were collected Linac Coherent Light Source (the first hard-X-ray laser user facility), which fitted a...
Abstract Access to experimental X-ray diffraction image data is fundamental for validation and reproduction of macromolecular models indispensable development structural biology processing methods. Here, we established a publication dissemination system, Structural Biology Data Grid (SBDG; data.sbgrid.org), preserve primary sets that support scientific publications. are accessible researchers through community driven grid, which facilitates global access. Our analysis pilot collection...
Abstract Battery function is determined by the efficiency and reversibility of electrochemical phase transformations at solid electrodes. The microscopic tools available to study chemical states matter with required spatial resolution specificity are intrinsically limited when studying complex architectures their reliance on two-dimensional projections thick material. Here, we report development soft X-ray ptychographic tomography, which resolves in three dimensions 11 nm resolution. We an...
We use extremely bright and ultrashort pulses from an x-ray free-electron laser (XFEL) to measure correlations in x rays scattered individual bioparticles. This allows us go beyond the traditional crystallography single-particle imaging approaches for structure investigations. employ angular recover three-dimensional (3D) of nanoscale viruses diffraction data measured at Linac Coherent Light Source. Correlations provide with a comprehensive structural fingerprint 3D virus, which we both...
Ever brighter light sources, fast parallel detectors and advances in phase retrieval methods have made ptychography a practical popular imaging technique. Compared to previous techniques, provides superior robustness resolution at the expense of more advanced time-consuming data analysis. By taking advantage massively architectures, high-throughput processing can expedite this analysis provide microscopists with immediate feedback. These allow real-time wavelength-limited resolution, coupled...