A5027691538- SARS-CoV-2 and COVID-19 Research
- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Microbial Community Ecology and Physiology
- COVID-19 Clinical Research Studies
- Protist diversity and phylogeny
- Neuropeptides and Animal Physiology
- Immunotherapy and Immune Responses
- Animal Virus Infections Studies
- Biochemical effects in animals
- Photosynthetic Processes and Mechanisms
- Hypothalamic control of reproductive hormones
- Monoclonal and Polyclonal Antibodies Research
- SARS-CoV-2 detection and testing
- vaccines and immunoinformatics approaches
- GABA and Rice Research
- Bacillus and Francisella bacterial research
- Regulation of Appetite and Obesity
- Estrogen and related hormone effects
- Viral gastroenteritis research and epidemiology
- Ion channel regulation and function
- Protein Kinase Regulation and GTPase Signaling
- Genomics and Phylogenetic Studies
Columbia University
2012-2025
Southern University of Science and Technology
2021-2025
Shenzhen Third People’s Hospital
2021-2025
National Clinical Research
2021-2022
Guangxi Veterinary Research Institute
2022
Columbia College
2019
Rutgers, The State University of New Jersey
2019
Royal College of Physicians
2019
XinHua Hospital
2017
Shanghai Jiao Tong University
2017
Human calcium-sensing receptor (CaSR) is a G-protein-coupled (GPCR) that maintains extracellular Ca2+ homeostasis through the regulation of parathyroid hormone secretion. It functions as disulfide-tethered homodimer composed three main domains, Venus Flytrap module, cysteine-rich domain, and seven-helix transmembrane region. Here, we present crystal structures entire domain CaSR in resting active conformations. We provide direct evidence L-amino acids are agonists receptor. In structure,...
The glycoprotein hormones of humans, produced in the pituitary and acting through receptors gonads to support reproduction thyroid gland for metabolism, have co-evolved from invertebrate counterparts
Abstract Access to experimental X-ray diffraction image data is fundamental for validation and reproduction of macromolecular models indispensable development structural biology processing methods. Here, we established a publication dissemination system, Structural Biology Data Grid (SBDG; data.sbgrid.org), preserve primary sets that support scientific publications. are accessible researchers through community driven grid, which facilitates global access. Our analysis pilot collection...
Abstract With declining SARS-CoV-2-specific antibody titers and increasing numbers of spike mutations, the ongoing emergence Omicron subvariants causes serious challenges to current vaccination strategies. BA.2 breakthrough infections have occurred in people who received wild-type vaccines, including mRNA, inactivated, or recombinant protein vaccines. Here, we evaluate evasion recently emerged BA.4/5 BA.2.75 two inactivated vaccine-immunized cohorts with infections. Compared neutralizing...
Significance The human calcium-sensing receptor maintains a stable concentration of calcium in the blood. Naturally occurring mutations are linked to homeostatic disorders including hypercalcemia and hypocalcemia. Modulation function can provide therapeutic relief these conditions. structures absence presence various allosteric modulators reveal mechanism by which modifiers up- or down-regulate function. We have captured symmetric forms homodimeric both at rest upon stimulation concluded...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron subvariants have seriously attacked the antibody barrier established by natural infection and/or vaccination, especially recently emerged BQ.1.1 and XBB.1. However, crucial mechanisms underlying virus escape broad neutralization remain elusive. Here, we present a panoramic analysis of broadly neutralizing activity binding epitopes 75 monoclonal antibodies isolated from prototype inactivated vaccinees. Nearly all (nAbs)...
Heterocysts, formed when filamentous cyanobacteria, such as Anabaena sp. strain PCC 7120, are grown in the absence of combined nitrogen, cells that specialized fixing atmospheric nitrogen (N(2)) under oxic conditions and transfer fixed to vegetative filament. mutants whose sepJ gene (open reading frame alr2338 genome) was affected showed filament fragmentation arrested heterocyst differentiation at an early stage. In a insertional mutant, layer similar polysaccharide formed, but...
Summary Photoreduction of dinitrogen by heterocyst‐forming cyanobacteria is great importance ecologically and for subsistence rice agriculture. Their heterocysts must have a glycolipid envelope layer that limits the entry oxygen if nitrogenase to remain active f ix in an ox ygen‐containing milieu (the Fox + phenotype). Genes alr5354 ( hglD ), alr5355 hglC ) alr5357 hglB filamentous cyanobacterium, Anabaena sp. strain PCC 7120, hglE Nostoc punctiforme are required synthesis heterocyst...
Metabotropic GABA B receptors mediate a significant fraction of inhibitory neurotransmission in the brain. Native receptor complexes contain principal subunits B1 and B2 , which form an obligate heterodimer, auxiliary subunits, known as potassium channel tetramerization domain-containing proteins (KCTDs). KCTDs interact with modify kinetics signaling. Little is about molecular mechanism governing direct association functional coupling these proteins. Here, we describe high-resolution...
SARS-CoV-2 infection widely induces antibody response targeting diverse viral proteins, including typical representative N-terminal domain (NTD), receptor-binding (RBD), and S2 subunit of spike. A lot NTD-, RBD-, S2-specific monoclonal antibodies (mAbs) have been isolated from COVID-19 convalescents, some which displaying potent activities to inhibit infection. However, a small portion NTD-specific mAbs elicited by wild-type (WT) primary could facilitate the virus entry into target cells in...
Several vaccines and immunization strategies, including inactivated vaccines, have proven effective in eliciting antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), providing an opportunity to characterize the antibody response. In this study, we investigated monoclonal responses elicited by wild-type SARS-CoV-2 vaccination compared those natural infection mRNA vaccination. The analysis showed that encoded biased germline genes were shared between vaccinated...
The eradication of tumors by the immune system depends on generation antigen-specific T cells which can migrate to sites tumor growth and maintain their effector functions despite local tumor-derived T-cell inhibitory factors. Interleukin-21 (IL-21) is an IL-2-related cytokine that has shown limited evidence antitumor activity in murine models early phase clinical trials. Effect IL-21 responses within microenvironment, however, not been extensively evaluated. Thus, we developed a stably...
Significance Human GABA B receptor transmits inhibitory signals in the brain. Defects are linked to several neurological diseases, including epilepsy and spasticity. The consists of two subunits, 1 (GBR1) 2 (GBR2). GBR1 is trapped inside cell because it contains an endoplasmic reticulum retention signal that targets intracellular compartment. This masked by coiled-coil interaction between GBR2 when subunits associate. crystal structure a complex reveals heterodimeric responsible for...
Inhibiting high-voltage-activated calcium channels (HVACCs; CaV1/CaV2) is therapeutic for myriad cardiovascular and neurological diseases. For particular applications, genetically-encoded HVACC blockers may enable channel inhibition with greater tissue-specificity versatility than achievable small molecules. Here, we engineered a inhibitor by first isolating an immunized llama nanobody (nb.F3) that binds auxiliary CaVβ subunits. Nb.F3 itself functionally inert, providing convenient vehicle...
Abstract The current COVID-19 pandemic caused by constantly emerging SARS-CoV-2 variants still poses a threat to public health worldwide. Effective next-generation vaccines and optimized booster vaccination strategies are urgently needed. Here, we sequentially immunized mice with wild-type inactivated vaccine heterologous mutant RBD vaccine, then evaluated their neutralizing antibody responses against including Beta, Delta, Alpha, Iota, Kappa, A.23.1. These data showed that third dose of...