M. Seibert
A5064491563- Advanced X-ray Imaging Techniques
- Photosynthetic Processes and Mechanisms
- Advanced Electron Microscopy Techniques and Applications
- Enzyme Structure and Function
- Spectroscopy and Quantum Chemical Studies
- Laser-Plasma Interactions and Diagnostics
- Astronomy and Astrophysical Research
- Photoreceptor and optogenetics research
- X-ray Spectroscopy and Fluorescence Analysis
- Stellar, planetary, and galactic studies
- Crystallization and Solubility Studies
- Astronomical Observations and Instrumentation
- X-ray Diffraction in Crystallography
- Crystallography and Radiation Phenomena
- Algal biology and biofuel production
- Light effects on plants
- Mitochondrial Function and Pathology
- Protein Structure and Dynamics
- Ion-surface interactions and analysis
- Particle Accelerators and Free-Electron Lasers
- Plant tissue culture and regeneration
- High-pressure geophysics and materials
- Astrophysical Phenomena and Observations
- Gamma-ray bursts and supernovae
- Laser-induced spectroscopy and plasma
Uppsala University
2011-2020
Linac Coherent Light Source
2011-2019
SLAC National Accelerator Laboratory
2007-2019
Carnegie Observatories
2016-2018
Carnegie Institution for Science
2018
Institute of Molecular Biology and Biophysics
2017
Technical University of Darmstadt
2017
National Renewable Energy Laboratory
1987-2016
Menlo School
2012-2014
Stanford Synchrotron Radiation Lightsource
2007-2014
Summary Microalgae represent an exceptionally diverse but highly specialized group of micro‐organisms adapted to various ecological habitats. Many microalgae have the ability produce substantial amounts (e.g. 20–50% dry cell weight) triacylglycerols (TAG) as a storage lipid under photo‐oxidative stress or other adverse environmental conditions. Fatty acids, building blocks for TAGs and all cellular lipids, are synthesized in chloroplast using single set enzymes, which acetyl CoA carboxylase...
Size Matters Less X-ray crystallography is a central research tool for uncovering the structures of proteins and other macromolecules. However, its applicability typically requires growth large crystals, in part because sufficient number molecules must be present lattice sample to withstand x-ray—induced damage. Boutet et al. (p. 362 , published online 31 May) now demonstrate that intense x-ray pulses emitted by free-electron laser source can yield data few enough exposures uncover...
X-ray crystallography of G protein-coupled receptors and other membrane proteins is hampered by difficulties associated with growing sufficiently large crystals that withstand radiation damage yield high-resolution data at synchrotron sources. We used an x-ray free-electron laser (XFEL) individual 50-femtosecond-duration pulses to minimize obtained a room-temperature structure human serotonin receptor using sub-10-micrometer microcrystals grown in mimetic matrix known as lipidic cubic phase....
The Trypanosoma brucei cysteine protease cathepsin B (TbCatB), which is involved in host protein degradation, a promising target to develop new treatments against sleeping sickness, fatal disease caused by this protozoan parasite. structure of the mature, active form TbCatB has so far not provided sufficient information for design safe and specific drug T. brucei. By combining two recent innovations, vivo crystallization serial femtosecond crystallography, we obtained room-temperature 2.1...
Intense femtosecond x-ray pulses produced at the Linac Coherent Light Source (LCLS) were used for simultaneous diffraction (XRD) and emission spectroscopy (XES) of microcrystals photosystem II (PS II) room temperature. This method probes overall protein structure electronic Mn4CaO5 cluster in oxygen-evolving complex PS II. XRD data are presented from both dark state (S1) first illuminated (S2) Our XRD-XES study shows that crystals intact during our measurements LCLS, not only with respect to...
We present a proof-of-concept three-dimensional reconstruction of the giant mimivirus particle from experimentally measured diffraction patterns an x-ray free-electron laser. Three-dimensional imaging requires assembly many two-dimensional into internally consistent Fourier volume. Since each is randomly oriented when exposed to pulse, relative orientations have be retrieved data alone. achieve this with modified version expand, maximize and compress algorithm validate our result using new...
We demonstrate the use of an X-ray free electron laser synchronized with optical pump to obtain diffraction snapshots from photoactivated states large membrane protein complexes in form nanocrystals flowing a liquid jet.Light-induced changes Photosystem I-Ferredoxin co-crystals were observed at time delays 5 10 µs after excitation.The result correlates microsecond kinetics transfer I ferredoxin.The undocking process that follows leads rearrangements crystals will terminally lead...
We present results from experiments at the Linac Coherent Light Source (LCLS) demonstrating that serial femtosecond crystallography (SFX) can be performed to high resolution (~2.5 Å) using protein microcrystals deposited on an ultra-thin silicon nitride membrane and embedded in a preservation medium room temperature. Data acquired acquisition rate x-ray free electron laser sources overcome radiation damage, while sample consumption is dramatically reduced compared flowing jet methods....
Abstract The new European X-ray Free-Electron Laser is the first free-electron laser capable of delivering pulses with a megahertz inter-pulse spacing, more than four orders magnitude higher previously possible. However, to date, it has been unclear whether would indeed be possible measure high-quality diffraction data at pulse repetition rates. Here, we show that structures can obtained using currently available operating conditions XFEL. We present two complete sets, one from well-known...
There exists a conspicuous gap of knowledge about the organization life at mesoscopic levels. Ultra-fast coherent diffractive imaging with X-ray free-electron lasers can probe structures relevant length scales and may reach sub-nanometer resolution on micron-sized living cells. Here we show that introduce beam aerosolised cyanobacteria into focus Linac Coherent Light Source record diffraction patterns from individual cells very low noise levels high hit ratios. We obtain two-dimensional...
In nanotechnology, strategies for the creation and manipulation of nanoparticles in gas phase are critically important surface modification substrate-free characterization. Recent coherent diffractive imaging with intense femtosecond X-ray pulses has verified capability single-shot nanoscale objects at suboptical resolutions beyond radiation-induced damage threshold. By intercepting electrospray-generated particles a single 15 soft-X-ray pulse, we demonstrate specimen free flight first time,...
The ultrafast evolution of microstructure is key to understanding high-pressure and strain-rate phenomena. However, the visualization lattice dynamics at scales commensurate with those atomistic simulations has been challenging. Here, we report femtosecond x-ray diffraction measurements unveiling response copper laser shock-compression peak normal elastic stresses ~73 gigapascals (GPa) strain rates 10(9) per second. We capture from a one-dimensional (1D) 3D plastically relaxed state within...