Martin Svenda
A5025633065- Advanced X-ray Imaging Techniques
- Advanced Electron Microscopy Techniques and Applications
- X-ray Spectroscopy and Fluorescence Analysis
- Laser-Plasma Interactions and Diagnostics
- Bacteriophages and microbial interactions
- Enzyme Structure and Function
- Plant Virus Research Studies
- Viral Infections and Vectors
- Astrophysical Phenomena and Observations
- Particle Accelerators and Free-Electron Lasers
- Ion-surface interactions and analysis
- Biochemical and Molecular Research
- Animal Virus Infections Studies
- Nanoplatforms for cancer theranostics
- Nanoparticle-Based Drug Delivery
- RNA and protein synthesis mechanisms
- Microbial Community Ecology and Physiology
- Electrohydrodynamics and Fluid Dynamics
- Coagulation and Flocculation Studies
- MRI in cancer diagnosis
- Microfluidic and Capillary Electrophoresis Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Particle Dynamics in Fluid Flows
- Orbital Angular Momentum in Optics
- Quantum Electrodynamics and Casimir Effect
KTH Royal Institute of Technology
2018-2025
Uppsala University
2011-2021
AlbaNova
2018-2021
SLAC National Accelerator Laboratory
2007-2019
Institute of Molecular Biology and Biophysics
2019
Swedish University of Agricultural Sciences
2011
Lawrence Livermore National Laboratory
2007
Stanford Synchrotron Radiation Lightsource
2007
We present a proof-of-concept three-dimensional reconstruction of the giant mimivirus particle from experimentally measured diffraction patterns an x-ray free-electron laser. Three-dimensional imaging requires assembly many two-dimensional into internally consistent Fourier volume. Since each is randomly oriented when exposed to pulse, relative orientations have be retrieved data alone. achieve this with modified version expand, maximize and compress algorithm validate our result using new...
There exists a conspicuous gap of knowledge about the organization life at mesoscopic levels. Ultra-fast coherent diffractive imaging with X-ray free-electron lasers can probe structures relevant length scales and may reach sub-nanometer resolution on micron-sized living cells. Here we show that introduce beam aerosolised cyanobacteria into focus Linac Coherent Light Source record diffraction patterns from individual cells very low noise levels high hit ratios. We obtain two-dimensional...
In nanotechnology, strategies for the creation and manipulation of nanoparticles in gas phase are critically important surface modification substrate-free characterization. Recent coherent diffractive imaging with intense femtosecond X-ray pulses has verified capability single-shot nanoscale objects at suboptical resolutions beyond radiation-induced damage threshold. By intercepting electrospray-generated particles a single 15 soft-X-ray pulse, we demonstrate specimen free flight first time,...
We use extremely bright and ultrashort pulses from an x-ray free-electron laser (XFEL) to measure correlations in x rays scattered individual bioparticles. This allows us go beyond the traditional crystallography single-particle imaging approaches for structure investigations. employ angular recover three-dimensional (3D) of nanoscale viruses diffraction data measured at Linac Coherent Light Source. Correlations provide with a comprehensive structural fingerprint 3D virus, which we both...
Single-particle experiments using X-ray Free Electron Lasers produce more than 10(5) snapshots per hour, consisting of an admixture blank shots (no particle intercepted), and exposures one or particles. Experimental data sets also often contain unintentional contamination with different species. We present unsupervised method able to sort experimental without recourse templates, specific noise models, user-directed learning. The results show 90% agreement manual classification.
Abstract Single particle diffractive imaging data from Rice Dwarf Virus (RDV) were recorded using the Coherent X-ray Imaging (CXI) instrument at Linac Light Source (LCLS). RDV was chosen as it is a well-characterized model system, useful for proof-of-principle experiments, system optimization and algorithm development. RDV, an icosahedral virus of about 70 nm in diameter, aerosolized injected into approximately 0.1 μm diameter focused hard beam CXI LCLS. Diffraction patterns with signal to...
This study explores the capabilities of Coherent X-ray Imaging Instrument at Linac Light Source to image small biological samples. The weak signal from samples puts a significant demand on experiment. Aerosolized Omono River virus particles ∼40 nm in diameter were injected into submicrometre focus reduced pressure. Diffraction patterns recorded two area detectors. statistical nature measurements many individual provided information about intensity profile beam, phase variations wavefront and...
The possibility of imaging single proteins constitutes an exciting challenge for x-ray lasers. Despite encouraging results on large particles, small particles has proven to be difficult two reasons: not quite high enough pulse intensity from currently available lasers and, as we demonstrate here, contamination the aerosolized molecules by nonvolatile contaminants in solution. amount sample depends initial droplet size during aerosolization. Here, show that, with our electrospray injector,...
Abstract Single-particle diffraction from X-ray Free Electron Lasers offers the potential for molecular structure determination without need crystallization. In an effort to further develop technique, we present a dataset of coherent soft images Coliphage PR772 virus, collected at Atomic Molecular Optics (AMO) beamline with pnCCD detectors in LAMP instrument Linac Coherent Light Source. The diameter ranges 65–70 nm, which is considerably smaller than previously reported ~600 nm Mimivirus....
The Pithoviridae giant virus family exhibits the largest viral particle known so far, a prolate spheroid up to 2.5 μm in length and 0.9 diameter. These particles show significant variations size. Little is about structure of intact virion due technical limitations with conventional electron cryo-microscopy (cryo-EM) when imaging thick specimens. Here we present Pithovirus sibericum at near native conditions using high-voltage cryo-tomography (cryo-ET) energy-filtered cryo-EM. We detected...
Diffraction before destruction using X-ray free-electron lasers (XFELs) has the potential to determine radiation-damage-free structures without need for crystallization. This article presents three-dimensional reconstruction of Melbournevirus from single-particle diffraction patterns collected at LINAC Coherent Light Source (LCLS) as well reconstructions simulated data exploring consequences different kinds experimental sources noise. The suffers a strong artifact in center particle. could...
Abstract Intense x-ray free-electron laser (XFEL) pulses hold great promise for imaging function in nanoscale and biological systems with atomic resolution. So far, however, the spatial resolution obtained from single shot experiments lags averaging static experiments. Here we report on a combined computational experimental study about ultrafast diffractive of sucrose clusters which are benchmark organic samples. Our theoretical model matches data water window to keV regime. The large-scale...
Nanoparticle (NP) based contrast agents detectable via different imaging modalities (multimodal properties) provide a promising strategy for noninvasive diagnostics. Core–shell NPs combining optical and X-ray fluorescence properties as bioimaging are presented. developed earlier computed tomography (XFCT), on ceramic molybdenum oxide (MoO2) metallic rhodium (Rh) ruthenium (Ru), coated with silica (SiO2) shell, using ethanolamine the catalyst. The SiO2 coating method introduced here is...
Soft X-ray microscopy (SXM) is a powerful technique for high-resolution biomedical imaging, enabling the observation of bio-nano interactions in near-native conditions without need heavy metal staining and fluorescence labeling. A laboratory soft microscope (LSXM) was developed to bridge resolution gap between light electron cellular imaging. However, LSXMs employ lower-brightness source comparison those operated synchrotron facilities, which can negatively affect contrast micrographs....
We describe femtosecond X-ray diffraction data sets of viruses and nanoparticles collected at the Linac Coherent Light Source.The establish first large benchmark for coherent methods freely available to public, bolster development algorithms that are essential developing this novel approach as a useful imaging technique.Applications 2D reconstructions, orientation classification finally 3D by assembling patterns into volume.
niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français étrangers, laboratoires publics privés.
The first hard X-ray laser, the Linac Coherent Light Source (LCLS), produces 120 shots per second.Particles injected into beam are hit randomly and in unknown orientations by extremely intense pulses, where femtosecond-duration pulses diffract from sample before particle structure is significantly changed even though ultimately destroyed deposited energy.Single diffraction experiments generate data at FEL repetition rate, resulting more than 400,000 detector readouts an hour, stream during...
Ultra-bright femtosecond X-ray pulses generated by free-electron lasers (XFELs) can be used to image high-resolution structures without the need for crystallization. For this approach, aerosol injection has been a successful method deliver 70–2000 nm particles into XFEL beam efficiently and at low noise. Improving technique of sample delivery extending it single proteins necessitates quantitative diagnostics. Here lab-based is introduced Rayleigh-scattering microscopy allowing us track size...
Omono River virus (OmRV) is a double-stranded RNA isolated from Culex mosquitos, and it belongs to group of unassigned insect viruses that appear be related Totiviridae. This paper describes electron cryo-microscopy (cryoEM) structures for the intact OmRV virion 8.9 Å resolution structure empty virus-like-particle, lacks RNA, 8.3 resolution. The icosahedral capsid contains 120-subunits resembles another closely arthropod-borne totivirus-like virus, infectious myonecrosis (IMNV) shrimps. Both...
A well-characterized wavefront is important for many x-ray free-electron laser (XFEL) experiments, especially single-particle imaging (SPI), where individual biomolecules randomly sample a nanometer region of highly focused femtosecond pulses. We demonstrate high-resolution multiple-plane an ensemble XFEL pulses, by Kirkpatrick–Baez mirrors, based on mixed-state ptychography, approach letting us infer and reduce experimental sources instability. From the recovered profiles, we show that...