A5051728165- Terahertz technology and applications
- Protein Structure and Dynamics
- Spectroscopy and Quantum Chemical Studies
- S100 Proteins and Annexins
- Advanced X-ray Imaging Techniques
- Photosynthetic Processes and Mechanisms
- X-ray Spectroscopy and Fluorescence Analysis
- Enzyme Structure and Function
- Advanced Chemical Physics Studies
- Molecular Biology Techniques and Applications
- Protease and Inhibitor Mechanisms
- RNA and protein synthesis mechanisms
- Peptidase Inhibition and Analysis
- Biochemical effects in animals
- Ion-surface interactions and analysis
- Agriculture and Biological Studies
- Gyrotron and Vacuum Electronics Research
- Social and Educational Sciences
- Magnetic and Electromagnetic Effects
- Mass Spectrometry Techniques and Applications
- Optical Coherence Tomography Applications
- Cardiac electrophysiology and arrhythmias
- Monoclonal and Polyclonal Antibodies Research
- Digital Holography and Microscopy
- Superconducting and THz Device Technology
Uppsala University
2018-2019
University of Gothenburg
2012-2019
Whether long-range quantum coherent states could exist in biological systems, and beyond low-temperature regimes where physics is known to be applicable, has been the subject debate for decades. It was proposed by Fröhlich that vibrational modes within protein molecules can order condense into a lowest-frequency mode process similar Bose-Einstein condensation, thus macroscopic coherence potentially observed systems. Despite prediction of these so-called condensates almost five decades ago,...
The possibility of imaging single proteins constitutes an exciting challenge for x-ray lasers. Despite encouraging results on large particles, small particles has proven to be difficult two reasons: not quite high enough pulse intensity from currently available lasers and, as we demonstrate here, contamination the aerosolized molecules by nonvolatile contaminants in solution. amount sample depends initial droplet size during aerosolization. Here, show that, with our electrospray injector,...
Diffraction before destruction using X-ray free-electron lasers (XFELs) has the potential to determine radiation-damage-free structures without need for crystallization. This article presents three-dimensional reconstruction of Melbournevirus from single-particle diffraction patterns collected at LINAC Coherent Light Source (LCLS) as well reconstructions simulated data exploring consequences different kinds experimental sources noise. The suffers a strong artifact in center particle. could...
S100A4 interacts with many binding partners upon Ca2+ activation and is strongly associated increased metastasis formation. In order to understand the role of C-terminal random coil for protein function we examined how small angle X-ray scattering wild-type its deletion mutant (residues 1–88, Δ13) changes binding. We found that intensity substantially in 0.15–0.25 Å−1 q-range whereas a similar change not visible C-terminus deleted mutant. Ensemble optimization SAXS modeling indicates entire...
Low-frequency vibrations are crucial for protein structure and function, but only a few experimental techniques can shine light on them. The main challenge when addressing dynamics in the terahertz domain is ubiquitous water that exhibit strong absorption. In this paper, we observe atoms directly using X-ray crystallography bovine trypsin at 100 K while irradiating crystals with 0.5 THz radiation alternating off states. We observed anisotropy of atomic displacements increased upon...
Photosynthetic reaction centers develop a stable charge separated state upon illumination. To investigate the molecular vibrations associated with illuminated of center we recorded terahertz absorption spectra photosynthetic from Rhodobacter sphaeroides in dark and illumination observed small, but significant THz increase 20 to 130 cm−1 spectral region. Reaction show very similar when solubilized detergents lipidic sponge phase indicating that nature bulk solvent has limited influence on...
In experimental research referencing two or more measurements to one another is a powerful tool reduce the effect of systematic errors between different sets measurements. The interesting quantity usually derived from on same sample under conditions. While an elaborate design essential for improving estimate, data analysis should also maximally exploit covariance X-ray crystallography difference structure-factor amplitudes carries important information solve phasing problems determine...
The possibility of imaging single proteins constitutes an exciting challenge for X-ray lasers. Despite encouraging results on large particles, small particles has proven to be difficult two reasons: not quite high enough pulse intensity from currently available lasers and, as we demonstrate here, contamination the aerosolised molecules by non-volatile contaminants in solution. amount sample depends initial droplet-size during aerosolisation. Here show that with our electrospray injector can...
We have periodically (25ms on - 25ms off) illuminated lysozyme crystals with 0.4 THz radiation and simultaneously monitored their X-ray diffraction intensity in order to study non-thermal structural changes the protein. In this work we analyze scaled unmerged observations using a multivariate Bayesian model improve accuracy of estimates. The pairs non-illuminated state show predominantly positive correlation. correlation decreases increasing resolution suggesting that finer slicing faster...
Abstract Low-frequency vibrations are crucial for protein structure and function, but only a few experimental techniques can shine light on them. The main challenge when addressing dynamics in the terahertz domain is ubiquitous water that exhibit strong absorption. In this paper, we observe atoms directly using X-ray crystallography bovine trypsin at 100 K while irradiating crystals with 0.5 THz radiation alternating off states. We observed anisotropy of atomic displacements increases upon...
S100A4 or metastasin plays a central role in the progression of metastatic diseases. Like many other examples, protein contains ordered and disordered regions. The aim this study was to determine how C‐terminal region influences binding Ca2+ turn Ca 2+ alters dynamics random coil. combination small angle X‐ray scattering, pulsed field gradient NMR measurements revealed conformational change upon wild‐type protein, but not deleted mutant. Comparative isothermal titration calorimetry also...
Correction for 'Terahertz absorption of illuminated photosynthetic reaction center solution: a signature photoactivation?' by Ida Lundholm et al., RSC Adv., 2014, 4, 25502–25509.