A5082282287- Enzyme Structure and Function
- Protein Structure and Dynamics
- Immunotherapy and Immune Responses
- Genomics and Chromatin Dynamics
- Glycosylation and Glycoproteins Research
- interferon and immune responses
- Monoclonal and Polyclonal Antibodies Research
- RNA and protein synthesis mechanisms
- T-cell and B-cell Immunology
- Epigenetics and DNA Methylation
- Meat and Animal Product Quality
- RNA modifications and cancer
- Microbial Community Ecology and Physiology
- Molecular Biology Techniques and Applications
- Identification and Quantification in Food
- thermodynamics and calorimetric analyses
- Mass Spectrometry Techniques and Applications
- Immune Cell Function and Interaction
- Milk Quality and Mastitis in Dairy Cows
- Spectroscopy and Quantum Chemical Studies
- Carbohydrate Chemistry and Synthesis
- Machine Learning in Bioinformatics
- Terahertz technology and applications
- Chromatin Remodeling and Cancer
- Biochemical effects in animals
University of Gothenburg
2016-2025
Sahlgrenska University Hospital
2024
Abstract Although virtual and augmented reality (VR AR) techniques have been used extensively in specialized laboratories, only recently did they become affordable, reaching wider consumer markets. With increased availability, it is timely to examine the roles that VR AR may play teaching structural biology experiencing complex data sets such as macromolecular structures. This guide suitable for those teachers of who do not a deep knowledge information technologies. study focuses on three...
Abstract The MUC2 mucin polymer is the main building unit of intestinal mucus layers separating microbiota from host epithelium. a large glycoprotein with C-terminal domain similar to MUC5AC and MUC5B mucins von Willebrand factor (VWF). A structural model part MUC2, MUC2-C, was generated by combining Cryo-electron microscopy, AlphaFold prediction, information its glycosylation, small angle X-ray scattering information. globular VWD4 assembly in N-terminal MUC2-C followed 3.5 linear VWC...
Mucus forms an important protective barrier that minimizes bacterial contact with the colonic epithelium. Intestinal mucus is organized in a complex network several specific proteins, including mucin-2 (MUC2) and abundant IgGFc-binding protein, FCGBP. FCGBP expressed all intestinal goblet cells secreted into mucus. It comprised of repeated von Willebrand D (vWD) domain assemblies, most which have GDPH amino acid sequence can be autocatalytically cleaved, as previously observed mucins MUC2...
Haloferax mediterranei, an extreme halophilic archaeon thriving in hypersaline environments, has acquired significant attention biotechnological and biochemical research due to its remarkable ability flourish salinity conditions. Transcription factors, essential regulating diverse cellular processes, have become focal points understanding adaptability. This study delves into the role of Lrp transcription factor, exploring modulation glnA, nasABC, lrp gene promoters vivo through...
Mucus in the colon is crucial for intestinal homeostasis by forming a barrier that separates microbes from epithelium. This achieved structural arrangement of major mucus proteins, such as MUC2 and FCGBP, both which are comprised several von Willebrand D domains (vWD) assemblies. Numerous disulfide bonds stabilise these domains, intermolecular generate multimers MUC2. The oligomeric nature FCGBP not known. Human hFCGBP contains 13 vWD whereas mouse mFCGBP consists only 7. We found unpaired...
Enterotoxigenic Escherichia coli (ETEC) infections are a leading cause of diarrheal illness, responsible for an estimated 100,000 deaths annually. ETEC pathogenesis is driven by various virulence factors, including toxins, adhesins, and noncanonical factors such as the protease EatA. The first line host defense against intestinal pathogenic bacterial protective mucus layer. Here, we demonstrate mechanism which EatA facilitates access to epithelial cell surface degrading core component MUC2,...
Abstract In the realm of atomic physics and chemistry, composition emerges as most powerful means describing matter. Mendeleev’s periodic table chemical formulas, while not entirely free from ambiguities, provide robust approximations for comprehending properties atoms, chemicals, their collective behaviours, which stem dynamic interplay constituents. Our study illustrates that protein-protein interactions follow a similar paradigm, wherein peptides plays pivotal role in predicting with...
Serial crystallography is a rapidly growing method that can yield structural insights from microcrystals were previously considered to be too small useful in conventional X-ray crystallography. Here, conditions for of the photosynthetic reaction centre Blastochloris viridis within lipidic cubic phase (LCP) crystallization matrix employ seeding protocol utilizing detergent-grown crystals with different crystal packing are described. LCP diffracted 2.25 Å resolution when exposed XFEL...
This study investigates the role of survivin in epigenetic control gene transcription through interaction with polycomb repressive complex 2 (PRC2). PRC2 is responsible for silencing expression by trimethylating lysine 27 on histone 3. We observed differential subunits CD4
In this study, we explore the role of nuclear survivin in maintaining effector phenotype IFNγ-producing T cells acting through transcriptional control glucose utilization. High expression CD4+T was associated with IFNγ-dependent and anaerobic glycolysis. Transcriptome CD4+ sequencing survivin-bound chromatin showed that had a genome-wide motif-specific binding to regulatory regions genes controlling cell metabolism. Survivin coprecipitates transcription factors IRF1 SMAD3, which repressed...
Single-wavelength X-ray anomalous diffraction (SAD) is a frequently employed technique to solve the phase problem in crystallography. The precision and accuracy of recovered differences are crucial for determining correct phases. Continuous rotation (CR) inverse-beam geometry (IBG) data collection methods have been performed on tetragonal lysozyme monoclinic survivin crystals analysis carried out how correlated pairs Friedel's reflections after scaling. A multivariate Bayesian model...
A biomolecular ensemble exhibits different responses to a temperature gradient depending on its diffusion properties. MicroScale Thermophoresis technique exploits this effect and is becoming popular for analyzing interactions of biomolecules in solution. When comparing affinities related compounds, the reliability determined thermodynamic parameters often comes into question. The thermophoresis binding curves can be assessed by Bayesian inference, which provides probability distribution...
Low-frequency vibrations are crucial for protein structure and function, but only a few experimental techniques can shine light on them. The main challenge when addressing dynamics in the terahertz domain is ubiquitous water that exhibit strong absorption. In this paper, we observe atoms directly using X-ray crystallography bovine trypsin at 100 K while irradiating crystals with 0.5 THz radiation alternating off states. We observed anisotropy of atomic displacements increased upon...
Haloferax mediterranei is an extremely halophilic archaeon, able to live in hypersaline environments with versatile nutritional requirements, whose study represents excellent basis the field of biotechnology. The transcriptional machinery Archaea combines eukaryotic basal apparatus and bacterial regulation mechanisms. However, little known about molecular mechanisms gene expression compared Bacteria, particularly Haloarchaea. genome Hfx. contains a gene, lrp (HFX_RS01210), which encodes...
In experimental research referencing two or more measurements to one another is a powerful tool reduce the effect of systematic errors between different sets measurements. The interesting quantity usually derived from on same sample under conditions. While an elaborate design essential for improving estimate, data analysis should also maximally exploit covariance X-ray crystallography difference structure-factor amplitudes carries important information solve phasing problems determine...
MicroScale Thermophoresis (MST) follows the movement of fluorescent-labelled biomolecules with different sizes along a temperature gradient.
Abstract This study explores a regulatory role of oncoprotein survivin on the bivalent regions chromatin (BvCR) characterized by concomitant deposition trimethylated lysine histone H3 at position 4 (H3K4me3) and 27 (H3K27me3). Intersect between BvCR sequences bound to demonstrated their co-localization cis -regulatory elements genes which execute DNA damage control in primary human CD4 + cells. Survivin anchored BRG1-complex repress repair IFNγ-stimulated In contrast, inhibition shifted...
Abstract In the realm of atomic physics and chemistry, composition emerges as most powerful means describing matter. Mendeleev’s periodic table chemical formulas, while not entirely free from ambiguities, provide robust approximations for comprehending properties atoms, chemicals, their collective behaviours, which stem dynamic interplay constituents. Our study illustrates that protein-protein interactions follow a similar paradigm, wherein peptides plays pivotal role in predicting with...