A5046121697- Photosynthetic Processes and Mechanisms
- Photoreceptor and optogenetics research
- Theoretical and Computational Physics
- Protein Structure and Dynamics
- Complex Systems and Time Series Analysis
- Porphyrin and Phthalocyanine Chemistry
- Statistical Mechanics and Entropy
- Enzyme Structure and Function
- Mass Spectrometry Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Medical Imaging Techniques and Applications
- Advanced MRI Techniques and Applications
- Spectroscopy and Quantum Chemical Studies
- Electrochemical Analysis and Applications
University of Gothenburg
2020-2024
European Synchrotron Radiation Facility
2022
University of Concepción
2018
Serial crystallography is a rapidly growing method that can yield structural insights from microcrystals were previously considered to be too small useful in conventional X-ray crystallography. Here, conditions for of the photosynthetic reaction centre Blastochloris viridis within lipidic cubic phase (LCP) crystallization matrix employ seeding protocol utilizing detergent-grown crystals with different crystal packing are described. LCP diffracted 2.25 Å resolution when exposed XFEL...
Cytochrome c oxidase (C O) is part of the respiratory chain and contributes to electrochemical membrane gradient in mitochondria as well many bacteria, it uses energy released reduction oxygen pump protons across an energy-transducing biological membrane. Here, we use time-resolved serial femtosecond crystallography study structural response active site upon flash photolysis carbon monoxide (CO) from reduced heme a 3 ba -type C O. In contrast with aa enzyme, our data show how CO stabilized...
With the development of serial crystallography at both x-ray free electron laser and synchrotron radiation sources, time-resolved is increasingly being applied to study conformational changes in macromolecules. A successful requires growth microcrystals, a mechanism for synchronized homogeneous excitation reaction interest within tools structural interpretation. Here, we utilize femtosecond data collected from microcrystals bacteriorhodopsin compare results partial occupancy refinement...
Time-resolved x-ray solution scattering (TR-XSS) is a sub-field of structural biology, which observes secondary changes in proteins as they evolve along their functional pathways. While the number distinct conformational states and rise decay can be extracted directly from TR-XSS experimental data recorded light-sensitive systems, modeling more challenging. This step often builds complementary information, including crystallographic studies or molecular dynamics simulations. When working...
Serial Femtosecond Crystallography (SFX) has revolutionised macromolecular crystallography by enabling X-ray diffraction data to be collected sequentially from continuous stream of microcrystals at room-temperature.Serial approaches have been successfully applied numerous time-resolved studies.Accurately assessing the quality and reliability time-dependent changes in electron density remains a challenge.To address this challenge, we developed "Xshuffle", Python-based toolbox designed for...