A5024573295- Light effects on plants
- Photosynthetic Processes and Mechanisms
- Biocrusts and Microbial Ecology
- Enzyme Structure and Function
- Microbial Community Ecology and Physiology
- Glycosylation and Glycoproteins Research
- Plant and Biological Electrophysiology Studies
- Bacterial Genetics and Biotechnology
- Protein Structure and Dynamics
- Photoreceptor and optogenetics research
- Bacteriophages and microbial interactions
- Algal biology and biofuel production
- Spectroscopy and Quantum Chemical Studies
- Protein purification and stability
- Microbial Metabolism and Applications
- Botanical Research and Applications
- Plant Gene Expression Analysis
- Social and Educational Sciences
- Hemoglobin structure and function
- Education, Healthcare and Sociology Research
- Porphyrin Metabolism and Disorders
- Genomics and Phylogenetic Studies
- Protist diversity and phylogeny
University of Gothenburg
2016-2022
University of Chicago
2019-2020
MAX IV Laboratory
2020
Lund University
2020
Northeastern Illinois University
2019
University of Wisconsin–Milwaukee
2019
Japan Synchrotron Radiation Research Institute
2019
SPring-8
2019
Kyoto University
2019
Phytochrome proteins control the growth, reproduction, and photosynthesis of plants, fungi, bacteria. Light is detected by a bilin cofactor, but it remains elusive how this leads to activation protein through structural changes. We present serial femtosecond X-ray crystallographic data chromophore-binding domains bacterial phytochrome at delay times 1 ps 10 after photoexcitation. The reveal twist D-ring, which partial detachment chromophore from protein. Unexpectedly, conserved so-called...
Many pathogenic bacteria utilise sialic acids as an energy source or use them external coating to evade immune detection. As such, that colonise sialylated environments deploy specific transporters mediate import of scavenged acids. Here, we report a substrate-bound 1.95 Å resolution structure and subsequent characterisation SiaT, acid transporter from Proteus mirabilis. SiaT is secondary active the sodium solute symporter (SSS) family, which Na
Abstract Phytochrome proteins detect red/far-red light to guide the growth, motion, development and reproduction in plants, fungi, bacteria. Bacterial phytochromes commonly function as an entrance signal two-component sensory systems. Despite availability of three-dimensional structures other proteins, conformational changes, which lead activation protein, are not understood. We reveal cryo electron microscopy complete phytochrome from Deinoccocus radiodurans its resting photoactivated...
Abstract Phytochromes are a family of photoreceptors that control light responses plants, fungi and bacteria. A sequence structural changes, which is not yet fully understood, leads to activation an output domain. Time-resolved serial femtosecond crystallography (SFX) can potentially shine on these conformational changes. Here we report the room temperature crystal structure chromophore-binding domains Deinococcus radiodurans phytochrome at 2.1 Å resolution. The was obtained by X-ray from...
Phytochromes are red-light photoreceptors that were first characterized in plants, with homologs photosynthetic and non-photosynthetic bacteria known as bacteriophytochromes (BphPs). Upon absorption of light, BphPs interconvert between two states denoted Pr Pfr distinct spectra the red far-red. They have recently been engineered enzymatic photoswitches for fluorescent-marker applications non-invasive tissue imaging mammals. This article presents cryo- room-temperature crystal structures...
Mammalian cell surfaces are decorated with complex glycoconjugates that terminate negatively charged sialic acids. Commensal and pathogenic bacteria can use host-derived acids for a competitive advantage, but require functional acid transporter to import the sugar into cell. This work investigates sodium symporter (SiaT) from Staphylococcus aureus (SaSiaT). We demonstrate SaSiaT rescues an Escherichia coli strain lacking its endogenous when grown on N-acetylneuraminic (Neu5Ac) or...
Phytochromes (PHYs) are photoreceptor proteins first discovered in plants, where they control a variety of photomorphogenesis events. PHYs as photochromic can reversibly switch between two distinct states: red light (Pr) and far-red (Pfr) absorbing form. The discovery Bacteriophytochromes (BphPs) nonphotosynthetic bacteria has opened new frontiers our understanding the mechanisms by which these natural photoswitches single cell development, although role BphPs vivo remains largely unknown....
The correct folding of proteins is paramount importance for their function, and protein misfolding believed to be the primary cause a wide range diseases. Protein has been investigated with time-averaged methods time-resolved spectroscopy, but observing structural dynamics unfolding process in real-time challenging. Here, we demonstrate an approach directly reveal changes reaction. We use nano- millisecond x-ray solution scattering probe apomyoglobin. reaction was triggered using temperature...
(6–4) photolyases are flavoproteins that belong to the photolyase/cryptochrome family. Their function is repair DNA lesions using visible light. Here, crystal structures of Drosophila melanogaster photolyase [ Dm (6–4)photolyase] at room and cryogenic temperatures reported. The room-temperature structure was solved 2.27 Å resolution obtained by serial femtosecond crystallography (SFX) an X-ray free-electron laser. crystallization preparation conditions also 1.79 conventional crystallography....
Phytochrome proteins control the growth, reproduction, and photosynthesis of plants, fungi, bacteria. Light is detected by a bilin cofactor, but it remains elusive how this leads to activation protein through structural changes. We present serial femtosecond X-ray crystallographic data chromophore-binding domains bacterial phytochrome at delay times 1 ps 10 after photoexcitation. The structures reveal twist D-ring, which lead partial detachment chromophore from protein. Unexpectedly,...
Abstract Phytochromes are red/far-red light photoreceptors in bacteria to plants, which elicit a variety of important physiological responses. They display reversible photocycle between the resting (dark) Pr state and activated Pfr state, signals received transduced as structural change through entire protein modulate activity protein. It is unknown how Pr-to-Pfr interconversion occurs structure intermediates remain notoriously elusive. Here, we present short-lived crystal structures...
Phytochromes are photoreceptor proteins that detect red and far-red light in plants, fungi bacteria.Light absorption by a bilin