A5044548477- Light effects on plants
- Advanced X-ray Imaging Techniques
- Photosynthetic Processes and Mechanisms
- X-ray Spectroscopy and Fluorescence Analysis
- Particle Accelerators and Free-Electron Lasers
- Advanced Electron Microscopy Techniques and Applications
- Enzyme Structure and Function
- Microbial Metabolism and Applications
- Plant and Biological Electrophysiology Studies
- Magnetic and Electromagnetic Effects
- Photoreceptor and optogenetics research
- Bacterial biofilms and quorum sensing
- Antimicrobial Resistance in Staphylococcus
Uppsala University
2024
University of Gothenburg
2022-2023
University of Veterinary Medicine
2020
Abstract Phytochrome proteins detect red/far-red light to guide the growth, motion, development and reproduction in plants, fungi, bacteria. Bacterial phytochromes commonly function as an entrance signal two-component sensory systems. Despite availability of three-dimensional structures other proteins, conformational changes, which lead activation protein, are not understood. We reveal cryo electron microscopy complete phytochrome from Deinoccocus radiodurans its resting photoactivated...
X-ray free-electron lasers (XFELs) can probe chemical and biological reactions as they unfold with unprecedented spatial temporal resolution. A principal challenge in this pursuit involves the delivery of samples to interaction point such a way that produces data highest possible quality maximal efficiency. This is hampered by intrinsic constraints posed light source operation within beamline environment. For liquid samples, solution typically some form high-speed jet, capable keeping up...
Abstract Detecting microsecond structural perturbations in biomolecules has wide relevance biology, chemistry and medicine. Here we show how MHz repetition rates at X-ray free-electron lasers can be used to produce time-series of protein scattering with exceptionally low noise levels 0.001%. We demonstrate the approach by examining Jɑ helix unfolding a light-oxygen-voltage photosensory domain. This time-resolved acquisition strategy is easy implement widely applicable for direct observation...
Highlights•Cryo-EM structures of a bathy bacteriophytochrome at near-atomic resolution•Large structural changes between states lead to strong on-off switching mechanism•Connector region length responsible for adjusting photoresponseSummaryPhytochromes are photoreceptor proteins in plants, fungi, and bacteria. They can adopt two photochromic with differential biochemical responses. The transducing the signal from chromophore output modules poorly understood due challenges capturing dynamic,...
Staphylococcosis is one of the most important infectious diseases in rabbit medicine, especially commercial farming. Previous studies revealed existence virulent variants adapted to rabbits. Typical and atypical, highly as well low have been isolated reported from industrial units all major rabbit-meat-producing countries. Preceding research focused on detecting defined nucleotide sequences, genome these organisms a whole was rarely subjected scientific investigations. The authors sequenced...
Summary Phytochromes are photoreceptor proteins in plants, fungi and bacteria. They can adopt two photochromic states with differential biochemical responses. The structural changes transducing the signal from chromophore to output modules poorly understood due challenges capturing structures of dynamic, full-length protein. Here, we present first cryo-electron microscopy phytochrome Pseudomonas aeruginosa ( Pa BphP) its resting Pfr photoactivated Pr state. kinase-active state has an...
<title>Abstract</title> Detecting microsecond structural perturbations in biomolecules has wide relevance biology, chemistry, and medicine. Here, we show how MHz repetition rates at X-ray free electron lasers (XFELs) can be used to produce time-series of protein scattering with exceptionally low noise levels 0.001%. We demonstrate the approach by deriving new mechanistic insight into Jα helix unfolding a Light-Oxygen-Voltage (LOV) photosensory domain. This time-resolved acquisition strategy...
X-ray Free Electron Lasers (XFELs) can probe chemical and biological reactions as they unfold with unprecedented spatial temporal resolution. A principal challenge in this pursuit is the delivery of samples to interaction point a way that produces data highest possible quality efficiency. This hampered by constraints posed light source operation within beamline environment. For liquid samples, solution typically involves high-speed jet, capable keeping up rate pulses. However, conventional...