A5092171926- Advanced X-ray Imaging Techniques
- Enzyme Structure and Function
- X-ray Spectroscopy and Fluorescence Analysis
- Advanced Electron Microscopy Techniques and Applications
- Particle Accelerators and Free-Electron Lasers
University of Gothenburg
2023
X-ray free-electron lasers (XFELs) can probe chemical and biological reactions as they unfold with unprecedented spatial temporal resolution. A principal challenge in this pursuit involves the delivery of samples to interaction point such a way that produces data highest possible quality maximal efficiency. This is hampered by intrinsic constraints posed light source operation within beamline environment. For liquid samples, solution typically some form high-speed jet, capable keeping up...
X-ray Free Electron Lasers (XFELs) can probe chemical and biological reactions as they unfold with unprecedented spatial temporal resolution. A principal challenge in this pursuit is the delivery of samples to interaction point a way that produces data highest possible quality efficiency. This hampered by constraints posed light source operation within beamline environment. For liquid samples, solution typically involves high-speed jet, capable keeping up rate pulses. However, conventional...
<title>Abstract</title> Detecting microsecond structural perturbations in biomolecules has wide relevance biology, chemistry, and medicine. Here, we show how MHz repetition rates at X-ray free electron lasers (XFELs) can be used to produce time-series of protein scattering with exceptionally low noise levels 0.001%. We demonstrate the approach by deriving new mechanistic insight into Jα helix unfolding a Light-Oxygen-Voltage (LOV) photosensory domain. This time-resolved acquisition strategy...