A5107960102- Biochemical and Molecular Research
- Drug Transport and Resistance Mechanisms
- Folate and B Vitamins Research
- Trace Elements in Health
- Bacterial Genetics and Biotechnology
- Endoplasmic Reticulum Stress and Disease
- Aldose Reductase and Taurine
- Bacteriophages and microbial interactions
- Glycosylation and Glycoproteins Research
- Legume Nitrogen Fixing Symbiosis
- Adenosine and Purinergic Signaling
- Enzyme Structure and Function
- Signaling Pathways in Disease
- Porphyrin Metabolism and Disorders
- Polyamine Metabolism and Applications
- melanin and skin pigmentation
- HIV/AIDS drug development and treatment
- Adolescent and Pediatric Healthcare
- Garlic and Onion Studies
- Cytomegalovirus and herpesvirus research
- Pneumocystis jirovecii pneumonia detection and treatment
- Lipid Membrane Structure and Behavior
- Amino Acid Enzymes and Metabolism
- Pharmacogenetics and Drug Metabolism
- Prenatal Substance Exposure Effects
National Chemical Laboratory
2024-2025
Academy of Scientific and Innovative Research
2024-2025
Institute for Stem Cell Biology and Regenerative Medicine
2018-2025
University of Gothenburg
2015-2019
VIB-VUB Center for Structural Biology
2014-2015
Institute of Structural and Molecular Biology
2015
Vrije Universiteit Brussel
2014-2015
International Centre for Genetic Engineering and Biotechnology
2014
N -Acetylneuraminic acid (Neu5Ac) is a negatively charged nine-carbon amino sugar that often the peripheral in human cell-surface glycoconjugates. Some bacteria scavenge, import, and metabolize Neu5Ac or redeploy it on their cell surfaces for immune evasion. The import of by many mediated tripartite ATP-independent periplasmic (TRAP) transporters. We have previously reported structures SiaQM, membrane-embedded component Haemophilus influenzae TRAP transport system, (Currie et al., 2024)....
Many pathogenic bacteria utilise sialic acids as an energy source or use them external coating to evade immune detection. As such, that colonise sialylated environments deploy specific transporters mediate import of scavenged acids. Here, we report a substrate-bound 1.95 Å resolution structure and subsequent characterisation SiaT, acid transporter from Proteus mirabilis. SiaT is secondary active the sodium solute symporter (SSS) family, which Na
Human dihydroorotate dehydrogenase ( hDHODH) catalyzes the rate-limiting step in de novo pyrimidine biosynthesis, conversion of to orotate. hDHODH has recently been found be associated with acute myelogenous leukemia, a disease for which standard intensive care not changed over decades. This work presents novel class inhibitors, are based on an unusual carboxylic group bioisostere 2-hydroxypyrazolo[1,5- a]pyridine, that designed starting from brequinar, one most potent inhibitors. A...
Aldo-keto reductase 1C3 (AKR1C3) is an attractive target in drug design for its role resistance to anticancer therapy. Several nonsteroidal anti-inflammatory drugs such as indomethacin are known inhibit AKR1C3 a nonselective manner because of COX-off effects. Here we designed two analogues by proposing bioisosteric connection between the carboxylic acid function and either hydroxyfurazan or hydroxy triazole rings. Both compounds were found selective manner. In particular, derivative highly...
Curli are functional amyloids expressed as fibres on the surface of Enterobacteriaceae. Contrary to protein misfolding events associated with pathogenic amyloidosis, curli result a dedicated biosynthetic pathway. A specialized transporter in outer membrane, CsgG, operates conjunction two accessory proteins CsgE and CsgF secrete curlin subunits extracellular surface, where they nucleate into cross-beta strand fibres. Here we investigate substrate tolerance CsgG capability heterologous...
Most amino acid substitutions in a protein either lead to partial loss-of-function or are near neutral. Several studies have shown the existence of second-site mutations that can rescue defects caused by diverse mutations. Such global suppressor key drivers evolution. However, mechanisms responsible for such suppression remain poorly understood. To address this, we characterized multiple both isolation and combination with inactive mutants. We examined six suppressors bacterial toxin CcdB,...
Abstract N -Acetylneuraminic acid (Neu5Ac) is a negatively charged nine-carbon amino-sugar that often the peripheral sugar in human cell-surface glycoconjugates. Some bacteria scavenge, import, and metabolize Neu5Ac, or they redeploy it on their cell surfaces for immune evasion. The import of Neu5Ac by many mediated tripartite ATP-independent periplasmic (TRAP) transporters. We have previously reported structures SiaQM, membrane-embedded component Haemophilus influenzae TRAP transport system...
N -Acetylneuraminic acid (Neu5Ac) is a negatively charged nine-carbon amino-sugar that often the peripheral sugar in human cell-surface glycoconjugates. Some bacteria scavenge, import, and metabolize Neu5Ac, or they redeploy it on their cell surfaces for immune evasion. The import of Neu5Ac by many mediated tripartite ATP-independent periplasmic (TRAP) transporters. We have previously reported structures SiaQM, membrane-embedded component Haemophilus influenzae TRAP transport system (Currie,...
Sialic acids comprise a varied group of nine-carbon amino sugars that are widely distributed among mammals and higher metazoans. Some human commensals bacterial pathogens can scavenge sialic from their environment degrade them for use as carbon nitrogen source. The enzyme N -acetylmannosamine kinase (NanK; EC 2.7.1.60) belongs to the transcriptional repressors, uncharacterized open reading frames sugar kinases (ROK) superfamily. NanK catalyzes second step acid catabolic pathway, transferring...
In Gram-negative bacteria, proteins destined for the extracellular environment or cell surface need to navigate 2 consecutive lipid bilayers. This can occur in a 2-step mechanism with separate carr...
N -Acetylneuraminic acid (Neu5Ac) is a negatively charged nine-carbon amino sugar that often the peripheral in human cell-surface glycoconjugates. Some bacteria scavenge, import, and metabolize Neu5Ac or redeploy it on their cell surfaces for immune evasion. The import of by many mediated tripartite ATP-independent periplasmic (TRAP) transporters. We have previously reported structures SiaQM, membrane-embedded component Haemophilus influenzae TRAP transport system, (Currie et al., 2024)....